Isolation and identification of phase 1 metabolites of demethoxycurcumin in rats. Drug Metab Dispos 35:1564–1573.

ABSTRACT: Curcuminoids are a safe natural food coloring additive with antiinflammatory, antioxidant, and anticarcinogenic activities. Although demethoxycurcumin is one of the major bioactive constituents of curcuminoids, knowledge about its metabolic fate is scant. In the present study, four new metabolites, 5-dehydroxyhexahydro-demethoxycurcumin-A (M- Curcuminoids are natural yellow pigments and food-coloring agents present in the rhizomes of the Asian tropical plant Curcuma longa, which has been used as a traditional medicinal herb for thousands of years. The dried rhizome of C. longa has been widely used as an aromatic stomachic, carminative, anthelmintic, laxative, as well as for liver ailments and as condiments in foods 1 Materials and Methods Materials. Dry rhizomes of C. longa were collected from Gui Zhou province, China. A voucher specimen was identified by Qi-Shi Sun (CL200209) and deposited at the Department of Natural Products Chemistry, Shenyang Pharmaceutical University, China. Demethoxycurcumin. Dry rhizomes of C. longa (2.5 kg) were pulverized and then extracted three times for 0.5 h/each time by ultrasound in an 8-fold volume (w/v) of 80% ethanol (EtOH). The EtOH solutions were combined and condensed to yield 362 g. Then the extract was chromatographed on a silica gel Article, publication date, and citation information can be found a