The Tropical Brown Alga Lobophora variegata: A Source of Antiprotozoal Compounds

Lobophora variegata, a brown alga collected from the coast of the Yucatan peninsula, Mexico, was studied for antiprotozoal activity against Giardia intestinalis, Entamoeba histolytica and Trichomonas vaginalis. The whole extract showed the highest activity against T. vaginalis, with an IC50 value of 3.2 μg/mL. For the fractions, the best antiprotozoal activity was found in non-polar fractions. The chloroform fraction of the extract contained a major sulfoquinovosyldiacylglycerol (SQDG), identified as 1-O-palmitoyl-2-O-myristoyl-3-O-(6‴-sulfo-α-d-quinovopyranosyl)-glycerol (1), together with small amounts of 1,2-di-O-palmitoyl-3-O-(6‴-sulfo-α-d-quinovopyranosyl)-glycerol (2) and a new compound identified as 1-O-palmitoyl-2-O-oleoyl-3-O-(6‴-sulfo-α-d-quinovopyranosyl)-glycerol (3). Their structures were elucidated on the basis of chemical and enzymatic hydrolysis and careful analysis of FAB-MS and NMR spectroscopic data. This is the first report on the isolation of SQDGs from L. variegata. The mixture of 1–3 showed good activity against E. histolytica and moderate activity against T. vaginalis with IC50s of 3.9 and 8.0 μg/mL, respectively, however, the activity of 1–3 is not as effective as metronidazole. These results afford ground information for the potential use of the whole extract and fractions of this species in protozoal infections

Trasplante intestinal experimental: aportaciones al modelo quirúrgico clásico y estudio de la tolerancia a través de la valoración de los hallazgos morfológicos en animales de larga supervivencia. Primer trasplante de intestino clínico en España

Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Medicina, Departamento de Cirugía. Fecha de lectura: 19 de Diciembre de 2005

Prevalencia de infección por Helicobacter Pylori en población pediátrica colombiana

Introducción: En los últimos años se ha reconocido un incremento en la prevalencia de infección por Helicobacter pylori en la población infantil, existen pocos estudios que establezcan estadísticas asociadas a la infección. Se pretendió establecer la prevalencia de infección por Helicobacter pylori en un periodo de dos años consecutivos. Metodología: Se realizó un estudio transversal incluyendo todos los pacientes con biopsia positiva para H. pylori en la Clínica Infantil Colsubsidio en los años 2013-2014. Resultados: Un total de 384 pacientes fueron incluidos. Se encontró una prevalencia de periodo de infección por H. pylori del 44.5% en la población pediátrica. El promedio de edad fue 6.9 ± 4 años. La prevalencia de periodo de HP fue mayor en el género femenino (27.86%), en región urbana (41.41%), y edad escolar (31.51%). El 32.44% de la población tenía endoscopia normal, con HP positivo. La mayor afectación fue en los pacientes de 6-14 años. El principal hallazgo endoscópico en pacientes con H. pylori positivo, en ambos géneros fue la gastritis crónica. La mayoría de los pacientes con infección por H. pylori se encontraron en adecuado estado nutricional. Discusión: Los datos encontrados no son concordantes con estadísticas mundiales. El presente estudio es un punto de partida para futuras investigaciones.Background: In recent years, it has been recognized an increase in the prevalence of Helicobacter pylori infection in children. There are few studies that establish statistics associated with this infection. It was intended to establish the prevalence of Helicobacter pylori infection in a period of two consecutive years. Methodology: A cross-sectional study was performed, including all patients with H. pylori positive biopsy in Clínica Infantil Colsubsidio in the years 2013-2014. Results: A total of 384 patients were included. The period prevalence of infection with H. pylori was found to be 44. 5% in the pediatric population. The average age was 6. 9 ± 4 years. HP period prevalence was higher in females (27. 86%), 41. 41% were from an urban area and 31. 51% were school age patients. 32. 44% of the population had normal endoscopy, with HP positive. The majority of patients affected were between the ages of 6-14 years. The main endoscopic finding in patients with H. pylori positive in both genders was chronic gastritis. Most patients with H. pylori infection were found in adequate nutritional status. Discussion: Data found is not similar to other studies worldwide. This is a point of departure for future research.Centro de investigaciones de Colsubsidio CINCOClínica Infantil ColsubsidioUniversidad del Rosari

Prevalencia de infección por Helicobacter Pylori en población pediátrica colombiana

Introducción: En los últimos años se ha reconocido un incremento en la prevalencia de infección por Helicobacter pylori en la población infantil, existen pocos estudios que establezcan estadísticas asociadas a la infección. Se pretendió establecer la prevalencia de infección por Helicobacter pylori en un periodo de dos años consecutivos. Metodología: Se realizó un estudio transversal incluyendo todos los pacientes con biopsia positiva para H. pylori en la Clínica Infantil Colsubsidio en los años 2013-2014. Resultados: Un total de 384 pacientes fueron incluidos. Se encontró una prevalencia de periodo de infección por H. pylori del 44.5% en la población pediátrica. El promedio de edad fue 6.9 ± 4 años. La prevalencia de periodo de HP fue mayor en el género femenino (27.86%), en región urbana (41.41%), y edad escolar (31.51%). El 32.44% de la población tenía endoscopia normal, con HP positivo. La mayor afectación fue en los pacientes de 6-14 años. El principal hallazgo endoscópico en pacientes con H. pylori positivo, en ambos géneros fue la gastritis crónica. La mayoría de los pacientes con infección por H. pylori se encontraron en adecuado estado nutricional. Discusión: Los datos encontrados no son concordantes con estadísticas mundiales. El presente estudio es un punto de partida para futuras investigaciones.Background: In recent years, it has been recognized an increase in the prevalence of Helicobacter pylori infection in children. There are few studies that establish statistics associated with this infection. It was intended to establish the prevalence of Helicobacter pylori infection in a period of two consecutive years. Methodology: A cross-sectional study was performed, including all patients with H. pylori positive biopsy in Clínica Infantil Colsubsidio in the years 2013-2014. Results: A total of 384 patients were included. The period prevalence of infection with H. pylori was found to be 44. 5% in the pediatric population. The average age was 6. 9 ± 4 years. HP period prevalence was higher in females (27. 86%), 41. 41% were from an urban area and 31. 51% were school age patients. 32. 44% of the population had normal endoscopy, with HP positive. The majority of patients affected were between the ages of 6-14 years. The main endoscopic finding in patients with H. pylori positive in both genders was chronic gastritis. Most patients with H. pylori infection were found in adequate nutritional status. Discussion: Data found is not similar to other studies worldwide. This is a point of departure for future research.Centro de investigaciones de Colsubsidio CINCOClínica Infantil ColsubsidioUniversidad del Rosari

Clinical Presentation and Anatopathologic Finding of a Hepatic Vascular Hamartoma: a Case Report.

HVH (hepatic vascular hamartoma) is a tumor like malformation arising from the vascular tissue of the liver. HVH has been previously reported in animals and presents distintive features from the most frequent benign tumor like malformation of the liver, the hepatic mesenchymal hamartoma (HMH). Herein we report a case of HVH localized in hepatic segment 4b, involving the gastro hepatic ligament, successfully treated with total excision. We describe the anatomo-pathologic findings focusing on the clinical and radiological presentation, the intraoperative characteristics and the differential diagnosis

Isolated recurrence of distal adenocarcinoma of the extrahepatic bile duct on a draining sinus scar after curative resection: Case Report and review of the literature

Abstract Background Surgical resection remains the gold standard for the treatment of localized adenocarcinoma of the extrahepatic bile ducts. Yet, treatment of loco-regional recurrences is not well defined. Case Presentation We present an unusual case of distal adenocarcinoma of the extrahepatic bile ducts that was treated with surgery and relapsed two years later with a solitary recurrence on the tract of a previous Redon drain. In addition, a review of the literature on management of loco regional relapses is presented. Conclusions The ideal management of these patients still remains undefined. Decisions are made based on clinical parameters from retrospective series, such as tumor grade, surgical margins or lymph node involvement. Prospective studies, that include molecular and genetic markers, are needed to improve patient selection and outcomes on this population.</p

Robot-assisted resection of gastrointestinal stromal tumors (GIST): a single center case series and literature review

Background: Robotic techniques are claimed to be an alternative to laparoscopic and open approaches for gastrointestinal stromal tumors (GIST) treatment. Our aim is to present our single center experience and a literature review. Materials and methods: From June 2012 to August 2014, six patients with preoperative diagnosis of GIST were treated by robotic surgery at Sanchinarro University Hospital. Results: Two GIST tumors were localized in the second part of the duodenum, one in the first portion, two in the gastricantrum and another in the angular notch. Surgical procedures performed were two subtotal gastrectomies, one gastric wedge resection and three duodenal enucleations. None of the interventions needed conversion to open surgery. Mean operative time was 245 min (150–540). Mean hospital stay was 10.5 days (6–24). All lesions had microscopically negative resection margins. Mean follow-up was 24 months (8–33) with a disease-free survival rate of 100%. Conclusions: A robotic approach for GIST tumors is a safe and feasible procedure with a well-accepted oncological surgical result