2 research outputs found
Contribution of platelet-derived microRNAs to serum microRNAs in healthy men
Platelets are a major source of microRNAs (miRNAs) in blood. Relationships between circulating platelet-derived miRNAs were investigated to elucidate their significance as biomarkers. Total miRNAs in serum were analyzed using the 3D-Gene miRNA Oligo chip. Among 22 miRNAs that are included in platelets and play functional roles, sufficient miRNA levels for comparison were detected for 11 miRNAs (let-7b-5p, miR-16β5p, miR-17β5p, miR-24β3p, miR-107, miR-126β3p, miR-150β3p, miR-191β5p, miR-197β3p, miR-223β3p, and miR-326). Among 55 pairs prepared by these miRNAs, relatively strong correlations (Spearmanβs correlation coefficient >0.8) were shown between miRNAs of 7 pairs including let-7b-5p and miR-16-5p, let-7b-5p and miR-17-5p, let-7b-5p and miR-107, miR-16β5p and miR-17-5p, miR-16-5p and miR-107, miR-17-5p and miR-107, and miR-107 and miR-126-3p. In principal component analysis, the first principal component consisted of let-7b-5p, miR-16-5p, miR-17-5p, miR-107, miR-126β3p, and miR-191-5p. These six miRNAs may be useful biomarkers that reflect platelet condition and function