41 research outputs found

    Potential role of LMP2 as tumor-suppressor defines new targets for uterine leiomyosarcoma therapy

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    Although the majority of smooth muscle neoplasms found in the uterus are benign, uterine leiomyosarcoma (LMS) is extremely malignant, with high rates of recurrence and metastasis. We earlier reported that mice with a homozygous deficiency for LMP2, an interferon (IFN)-gamma-inducible factor, spontaneously develop uterine LMS. The IFN-gamma pathway is important for control of tumor growth and invasion and has been implicated in several cancers. In this study, experiments with human and mouse uterine tissues revealed a defective LMP2 expression in human uterine LMS that was traced to the IFN-gamma pathway and the specific effect of JAK-1 somatic mutations on the LMP2 transcriptional activation. Furthermore, analysis of a human uterine LMS cell line clarified the biological significance of LMP2 in malignant myometrium transformation and cell cycle, thus implicating LMP2 as an anti-tumorigenic candidate. This role of LMP2 as a tumor suppressor may lead to new therapeutic targets in human uterine LMS.ArticleSCIENTIFIC REPORTS. 1:180 (2011)journal articl

    Solution X-ray Scattering Study of Reconstitution Process of Tobacco Mosaic Virus Particle Using Low-Temperature Quenching (MOLECULAR BIOLOGY AND INFORMATION-Biopolymer Structure)

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    The reconstitution process of tobacco mosaic virus (TMV) was investigated by the solution X-ray scattering measurements with the synchrotron radiation source using low-temperature quenching. TMV assembly in an aqueous solution is completely stopped below 5°C. The TMV assembly was traced by the small-angle X-ray scattering (SAXS) measurements at 5°C on a series of solutions prepared by low-temperature quenching after incubation either at 15, 20 or 25°C appropriate interval between 0 and 60 min. The SAXS results were analyzed by the Guinier plot, the Kratky plot and the distant distribution function. In order to account the time course of SAXS profiles in terms of the elongation of TMV assembly, a model calculation was performed by applying Glatter's multibody method. The simulated model functions support the conclusion that the incubation of the RNA and protein of TMV began to reconstitute TMV instantly after mixing, proceeded steeply to a long rod

    Serviceological View of the Development of a Person Trip Survey Application

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    Abstract We are planning to introduce a new public transportation system, called the Smart Access Vehicle (SAV) system, to Hakodate city. For this purpose, since November 2012 we have been conducting a PT survey in which people run the dedicated smartphone application that we are developing. The process of developing the application forms the so-called 'design-service loop', consisting of design by simulation, implementation in the real world, and analysing/modelling. Up to now, we have iterated the loo
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