Relationships between Spatial and Temporal Variations in Precipitation, Climatic Indices, and the Normalized Differential Vegetation Index in the Upper and Middle Reaches of the Heihe River Basin, Northwest China

Changes in precipitation are critical indicators of climate change. In this study, the daily precipitation records from 10 meteorological stations in the Heihe River Basin, Northwest China from 1961–2016, precipitation indices, climate indices, and the normalized differential vegetation index (NDVI) were investigated using the Pearson, Kendall, and Spearman correlation coefficients; Theil-Sen Median; Mann–Kendall test; and wavelet coherence. The results indicated that the occurrences (fractional contributions) of 1–2-day wet periods were 81.3% (93.9%) and 55.3% (82.1%) in the upper (UHRB) and middle (MHRB) reaches of the Heihe River Basin, respectively. The spatial distribution of the occurrences (fractional contributions) was almost consistent with non-significant increases/decreases at stations. The ATP, ATD, API, and AMRD increased, while precipitation regimes suggest that dry seasons are getting wetter, and wet seasons are getting drier, although these changes were not significant. Wavelet coherence analyses showed that climate indices influenced precipitation, mainly its concentration, on a 4- to 78.6-month timescale. The Pearson, Kendall, and Spearman correlation coefficients showed weak lagged linkages between precipitation and the North Arctic Oscillation (NAO), Pacific Decadal Oscillation (PDO), and Atlantic Multidecadal Oscillation (AMO). The NDVI of grasslands, meadows and coniferous forests was significantly and positively correlated with precipitation, while the NDVI of alpine vegetation, swamps and shrubs was negatively and significantly correlated with precipitation in the UHRB. The NDVI of grasslands was significantly and positively correlated, but the NDVI of shrubs, coniferous forests and cultivated vegetation was negatively and significantly correlated with precipitation in the MHRB. The correlation between cultivated vegetation and natural precipitation in the MHRB may have been weakened by human activities

Lysine acetylation contributes to development, aflatoxin biosynthesis and pathogenicity in Aspergillus flavus

Aspergillus flavus is a pathogenic fungus that produces carcinogenic aflatoxins, posing a great threat to crops, animals and humans. Lysine acetylation is one of the most important reversible post-translational modifications and plays a vital regulatory role in various cellular processes. However, current information on the extent and function of lysine acetylation and aflatoxin biosynthesis in A. flavus is limited. Here, a global acetylome analysis of A. flavus was performed by peptide pre-fractionation, pan-acetylation antibody enrichment and liquid chromatography-mass spectrometry. A total of 1313 high-confidence acetylation sites in 727 acetylated proteins were identified in A. flavus. These acetylation proteins are widely involved in glycolysis/gluconeogenesis, pentose phosphate pathway, citric acid cycle and aflatoxin biosynthesis. AflO (O-methyltransferase), a key enzyme in aflatoxin biosynthesis, was found to be acetylated at K241 and K384. Deletion of aflO not only impaired conidial and sclerotial developments, but also dramatically suppressed aflatoxin production and pathogenicity of A. flavus. Further site-specific mutations showed that lysine acetylation of AflO could also result in defects in development, aflatoxin production and pathogenicity, suggesting that acetylation plays a vital role in the regulation of the enzymatic activity of AflO in A. flavus. Our findings provide evidence for the involvement of lysine acetylation in various biological processes in A. flavus and facilitating in the elucidation of metabolic networks