Electron self-energy and effective mass in a single heterostructure

In this paper, we investigate the electron self-energy and effective mass in a single heterostructure using Green-function method. Numerical calculations of the electron self-energy and effective mass for GaAs/AlAs heterostructure are performed. The results show that the self energy (effective mass) of electron, which incorporate the energy of electron coupling to interface-optical phonons and half three-dimension LO phonons, monotonically increase(decrease) from that of interface polaron to that of 3D bulk polaron with the increase of the distance between the position of the electron and interface.Comment: 10 pages, 2 figure

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One-step implementation of multi-qubit conditional phase gating with nitrogen-vacancy centers coupled to a high-Q silica microsphere cavity

The diamond nitrogen-vacancy (NV) center is an excellent candidate for quantum information processing, whereas entangling separate NV centers is still of great experimental challenge. We propose an one-step conditional phase flip with three NV centers coupled to a whispering-gallery mode cavity by virtue of the Raman transition and smart qubit encoding. As decoherence is much suppressed, our scheme could work for more qubits. The experimental feasibility is justified.Comment: 3 pages, 2 figures, Accepted by Appl. Phys. Let

Genome replication engineering assisted continuous evolution (GREACE) to improve microbial tolerance for biofuels production

BACKGROUND: Microbial production of biofuels requires robust cell growth and metabolism under tough conditions. Conventionally, such tolerance phenotypes were engineered through evolutionary engineering using the principle of “Mutagenesis followed-by Selection”. The iterative rounds of mutagenesis-selection and frequent manual interventions resulted in discontinuous and inefficient strain improvement processes. This work aimed to develop a more continuous and efficient evolutionary engineering method termed as “Genome Replication Engineering Assisted Continuous Evolution” (GREACE) using “Mutagenesis coupled-with Selection” as its core principle. RESULTS: The core design of GREACE is to introduce an in vivo continuous mutagenesis mechanism into microbial cells by introducing a group of genetically modified proofreading elements of the DNA polymerase complex to accelerate the evolution process under stressful conditions. The genotype stability and phenotype heritability can be stably maintained once the genetically modified proofreading element is removed, thus scarless mutants with desired phenotypes can be obtained. Kanamycin resistance of E. coli was rapidly improved to confirm the concept and feasibility of GREACE. Intrinsic mechanism analysis revealed that during the continuous evolution process, the accumulation of genetically modified proofreading elements with mutator activities endowed the host cells with enhanced adaptation advantages. We further showed that GREACE can also be applied to engineer n-butanol and acetate tolerances. In less than a month, an E. coli strain capable of growing under an n-butanol concentration of 1.25% was isolated. As for acetate tolerance, cell growth of the evolved E. coli strain increased by 8-fold under 0.1% of acetate. In addition, we discovered that adaptation to specific stresses prefers accumulation of genetically modified elements with specific mutator strengths. CONCLUSIONS: We developed a novel GREACE method using “Mutagenesis coupled-with Selection” as core principle. Successful isolation of E. coli strains with improved n-butanol and acetate tolerances demonstrated the potential of GREACE as a promising method for strain improvement in biofuels production