Integrate CRISPR/Cas9 for protein expression of HLA-B*38:68Q via precise gene editing.

The determination of null- or low-expressed HLA alleles is clinically relevant in both hematopoietic stem cell transplantation and solid organ transplantation. We studied the expression level of a questionable (Q) HLA-B*38:68Q allele, which carries a 9-nucleotide (nt) deletion at codon 230-232 in exon 4 of HLA-B*38:01:01:01 using CRISPR/Cas9 gene editing technology. CRISPR/Cas9 gene editing of HLA-B*38:01:01:01 homozygous EBV B cell line resulted in one HLA-B*38:68Q/B*38:01:01:01 heterozygous and one HLA-B*38:68Q homozygous clone. Flow cytometric analysis of monoclonal anti-Bw4 antibody showed the protein expression of HLA-B*38:01:01:01 in homozygous cells was 2.2 fold higher than HLA-B*38:68Q/B*38:01:01:01 heterozygous cells, and the expression of HLA-B*38:68Q/B*38:01:01:01 heterozygous cells was over 2.0 fold higher than HLA-B*38:68Q homozygous cells. The HLA-B*38:68Q expression was further confirmed using anti-B38 polyclonal antibody. Similarly, the expression of the HLA-B*38:01:01:01 homozygous cells was 1.5 fold higher than that of HLA-B*38:68Q/B*38:01:01:01 heterozygous cells, and the HLA-B*38:68Q/B*38:01:01:01 heterozygous cells was over 1.6 fold higher than that of HLA-B*38:68Q homozygous cells. The treatment of HLA-B*38:68Q homozygous cells with IFN-γ significantly increased its expression. In conclusion, we demonstrate that HLA-B*38:68Q is a low-expressing HLA allele. The CRISPR/Cas9 technology is a useful tool to induce precise gene editing in HLA genes to enable the characterization of HLA gene variants on expression and function

Measuring the contribution of the ocean: A comparison of the statistical classification of the marine economy used by China and Canada

Most of the major marine countries share an identical knowledge about marine economy. Ocean-related principle is the primary principles which distinguish the ocean economy from national economy and other economies. The understandings of marine economy from various countries all take into consideration the ocean-relativeness character geographically or industrially. However, there are certain differences in statistical frameworks and specific industrial classifications. In this paper, the statistical classification of marine economy between China and Canada is comparatively studied from the perspectives of the connotation of marine economy, the classification of regional statistics, and the classification of industrial statistics. Moreover, the identification of the statistical calibers of the two countries’ marine economy is further analyzed. This allows for a comparison of the statistical data between the two countries’ marine economy. Several suggestions on enforcing the statistical work for the marine economy are proposed in the end

Human MicroRNA Oncogenes and Tumor Suppressors Show Significantly Different Biological Patterns: From Functions to Targets

MicroRNAs (miRNAs) are small noncoding RNAs which play essential roles in many important biological processes. Therefore, their dysfunction is associated with a variety of human diseases, including cancer. Increasing evidence shows that miRNAs can act as oncogenes or tumor suppressors, and although there is great interest in research into these cancer-associated miRNAs, little is known about them. In this study, we performed a comprehensive analysis of putative human miRNA oncogenes and tumor suppressors. We found that miRNA oncogenes and tumor suppressors clearly show different patterns in function, evolutionary rate, expression, chromosome distribution, molecule size, free energy, transcription factors, and targets. For example, miRNA oncogenes are located mainly in the amplified regions in human cancers, whereas miRNA tumor suppressors are located mainly in the deleted regions. miRNA oncogenes tend to cleave target mRNAs more frequently than miRNA tumor suppressors. These results indicate that these two types of cancer-associated miRNAs play different roles in cancer formation and development. Moreover, the patterns identified here can discriminate novel miRNA oncogenes and tumor suppressors with a high degree of accuracy. This study represents the first large-scale bioinformatic analysis of human miRNA oncogenes and tumor suppressors. Our findings provide help for not only understanding of miRNAs in cancer but also for the specific identification of novel miRNAs as miRNA oncogenes and tumor suppressors. In addition, the data presented in this study will be valuable for the study of both miRNAs and cancer

The mediating role of dynamic capability between sustainable development and competitive advantage in tourism enterprises in Henan Province (China)

Objective: This study explores the structural relationships between sustainable development and competitive advantage in tourism enterprises, along with the mediating role of dynamic capability, especially following the impact of COVID-19. Design/Method/Approach: An online questionnaire survey was conducted to collect 430 valid primary data sets from middle and senior managers in Henan tourism enterprises. Covariance-based structural equation modeling was utilized to test the direct effect, while the bootstrap method was used for testing mediating effects. Findings: The results reveal that sustainable development has a significant impact on the competitive advantage of tourism enterprises from three sustainability dimensions: economic, environmental and social. Meanwhile, dynamic capabilities represented by adaptive, absorptive and innovative capacities play a mediating role between them. Conclusions/Recommendation: The reshaping of the tourism industry by COVID-19 requires practitioners to take dynamic capability seriously in an uncertain environment. The higher demand for sustainability from tourists after the pandemic also suggests that improvements are a viable way for tourism enterprises to restore and cultivate competitive advantage. Originality/ Value: This study focuses on public health emergencies which are scarce in tourism crisis research (Duan et al., 2021). In addition, it is designed to help managers identify elements that can be used as internal drivers (Wang, 2021) showing how firms can incorporate sustainable development into strategic management, as this is still lacking (Zhang et al., 2020) in practice

cisPath: an R/Bioconductor package for cloud users for visualization and management of functional protein interaction networks

Background: With the burgeoning development of cloud technology and services, there are an increasing number of users who prefer cloud to run their applications. All software and associated data are hosted on the cloud, allowing users to access them via a web browser from any computer, anywhere. This paper presents cisPath, an R/Bioconductor package deployed on cloud servers for client users to visualize, manage, and share functional protein interaction networks. Results: With this R package, users can easily integrate downloaded protein-protein interaction information from different online databases with private data to construct new and personalized interaction networks. Additional functions allow users to generate specific networks based on private databases. Since the results produced with the use of this package are in the form of web pages, cloud users can easily view and edit the network graphs via the browser, using a mouse or touch screen, without the need to download them to a local computer. This package can also be installed and run on a local desktop computer. Depending on user preference, results can be publicized or shared by uploading to a web server or cloud driver, allowing other users to directly access results via a web browser. Conclusions: This package can be installed and run on a variety of platforms. Since all network views are shown in web pages, such package is particularly useful for cloud users. The easy installation and operation is an attractive quality for R beginners and users with no previous experience with cloud services.SCI(E)CPCI-S(ISTP)[email protected]

Analysis and Design of Wind Turbine Monitoring System Based on Edge Computing

INTRODUCTION: A wind turbine data analysis method based on the combination of Hadoop and edge computing is proposed. OBJECTIVES: Solve the wind turbine health status monitoring system large data, time extension, energy consumption and other problems. METHODS: By analysing the technical requirements and business processes of the system, the overall framework of the system was designed and a deep reinforcement learning algorithm based on big data was proposed. RESULTS: It solves the problem of insufficient computing resources as well as energy consumption and latency problems occurring in the data analysis layer, solves the problems in WTG task offloading, and improves the computational offloading efficiency of the edge nodes to complete the collection, storage, and analysis of WTG data. CONCLUSION: The data analysis and experimental simulation platform is built through Python, and the results show that the application of Hadoop and the edge computing offloading strategy based on the DDPG algorithm to the system improves the system's quality of service and computational performance, and the method is applicable to the distributed storage and analysis of the device in the massive monitoring data

Sentinel lymph node biopsy in oral cavity cancer using indocyanine green: A systematic review and meta-analysis

This meta-analysis was conducted to evaluate the value of indocyanine green (ICG) in guiding sentinel lymph node biopsy (SLNB) for patients with oral cavity cancer. An electronic database search (PubMed, MEDLINE, Cochrane Library, Embase, and Web of Science) was performed from their inception to June 2020 to retrieve clinical studies of ICG applied to SLNB for oral cavity cancer. Data were extracted from 14 relevant articles (226 patients), and 9 studies (134 patients) were finally included in the meta-analysis according to the inclusion and exclusion criteria. The pooled sentinel lymph node (SLN) sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, and diagnostic odds ratio were 88.0% (95% confidence interval [CI], 74.0-96.0), 64.0% (95% CI, 61.0-66.0), 2.45 (95% CI, 1.31-4.60), 0.40 (95% CI, 0.17-0.90), and 7.30 (95% CI, 1.74-30.68), respectively. The area under the summary receiver operating characteristic curve was 0.8805. In conclusion, ICG applied to SLNB can effectively predict the status of regional lymph nodes in oral cavity cancer