Single-cell sequencing of genomic DNA resolves sub-clonal heterogeneity in a melanoma cell line

Abstract: We performed shallow single-cell sequencing of genomic DNA across 1475 cells from a cell-line, COLO829, to resolve overall complexity and clonality. This melanoma tumor-line has been previously characterized by multiple technologies and is a benchmark for evaluating somatic alterations. In some of these studies, COLO829 has shown conflicting and/or indeterminate copy number and, thus, single-cell sequencing provides a tool for gaining insight. Following shallow single-cell sequencing, we first identified at least four major sub-clones by discriminant analysis of principal components of single-cell copy number data. Based on clustering, break-point and loss of heterozygosity analysis of aggregated data from sub-clones, we identified distinct hallmark events that were validated within bulk sequencing and spectral karyotyping. In summary, COLO829 exhibits a classical Dutrillaux’s monosomic/trisomic pattern of karyotype evolution with endoreduplication, where consistent sub-clones emerge from the loss/gain of abnormal chromosomes. Overall, our results demonstrate how shallow copy number profiling can uncover hidden biological insights

Joint analysis of three genome-wide association studies of esophageal squamous cell carcinoma in Chinese populations

We conducted a joint (pooled) analysis of three genome-wide association studies (GWAS) 1-3 of esophageal squamous cell carcinoma (ESCC) in ethnic Chinese (5,337 ESCC cases and 5,787 controls) with 9,654 ESCC cases and 10,058 controls for follow-up. In a logistic regression model adjusted for age, sex, study, and two eigenvectors, two new loci achieved genome-wide significance, marked by rs7447927 at 5q31.2 (per-allele odds ratio (OR) = 0.85, 95% CI 0.82-0.88; P=7.72x10−20) and rs1642764 at 17p13.1 (per-allele OR= 0.88, 95% CI 0.85-0.91; P=3.10x10−13). rs7447927 is a synonymous single nucleotide polymorphism (SNP) in TMEM173 and rs1642764 is an intronic SNP in ATP1B2, near TP53. Furthermore, a locus in the HLA class II region at 6p21.32 (rs35597309) achieved genome-wide significance in the two populations at highest risk for ESSC (OR=1.33, 95% CI 1.22-1.46; P=1.99x10−10). Our joint analysis identified new ESCC susceptibility loci overall as well as a new locus unique to the ESCC high risk Taihang Mountain region

Cooperative conflict detection and resolution of civil unmanned aerial vehicles in metropolis

Unmanned air vehicles have recently attracted attention of many researchers because of their potential civil applications. A systematic integration of unmanned air vehicles in non-segregated airspace is required that allows safe operation of unmanned air vehicles along with other manned aircrafts. One of the critical issues is conflict detection and resolution. This article proposes to solve unmanned air vehicles’ conflict detection and resolution problem in metropolis airspace. First, the structure of metropolis airspace in the coming future is studied, and the airspace conflict problem between different unmanned air vehicles is analyzed by velocity obstacle theory. Second, a conflict detection and resolution framework in metropolis is proposed, and factors that have influences on conflict-free solutions are discussed. Third, the multi-unmanned air vehicle conflict resolution problem is formalized as a nonlinear optimization problem with the aim of minimizing overall conflict resolution consumption. The safe separation constraint is further discussed to improve the computation efficiency. When the speeds of conflict-involved unmanned air vehicles are equal, the nonlinear safe separation constraint is transformed into linear constraints. The problem is solved by mixed integer convex programming. When unmanned air vehicles are with unequal speeds, we propose to solve the nonlinear optimization problem by stochastic parallel gradient descent–based method. Our approaches are demonstrated in computational examples

A Cross-Domain Alliance Authentication Scheme based on Bilinear Group

Abstract: With the development of grid computing, cloud computing and other large distributed network technology, users need them to provide services of unlimited space and unlimited speed. In order to meeting this request of users, all the domains in these large distributed networks need coordination for each other. For ensuring the safety to access resources in all domains, we propose a crossdomain union authentication scheme. We compute a large prime cyclic group by elliptic curve, and use the direct decomposition of this group to decompose automorphism groups,and design an signcryption scheme between domains by bilinear of automorphism group to achieve cross-domain union authentication. This scheme overcome the complexity of certificate transmission and bottlenecks in the scheme of PKI-based, and it can trace the entities and supports two-way entities anonymous authentication, which avoid the domain certificate authority counterfeiting its member to access cross-domain resources. Analyses show that its advantages on security and communication-consumption

Giant right atrial cystic hamartoma: a case report and literature review

An 11-year-old boy presenting with palpitation and chest distress was found to have gross cardiomegaly on chest radiography. Subsequent echocardiography revealed an intramural giant cystic mass in the right atrium. An operative measure was planned to prevent acute cardiac tamponade and right coronary artery obstruction. The patient successfully underwent open cystectomy to remove the mass, which was located on the front wall of the right atrium and extended to the atrioventricular appendage. Histopathological examination confirmed a cystic hamartoma. To the best of our knowledge, this is the first reported case of a giant cystic hamartoma located on the right atrium