Reference intervals for thyroid disorders calculated by indirect method and comparison with reference change values

IntroductionThe aim of the study was to calculate reference intervals (RIs) for thyroid stimulating hormone (TSH), free thyroxine (fT4) and free triiodothyronine (fT3) and evaluate the clinical significance of these intervals by use of reference change values (RCV) of the analytes. Materials and methodsLaboratory patient data between August and December 2021 were evaluated for the study. A total of 188,912 patients with TSH, fT4, fT3, anti-thyroid peroxidase antibodies (Anti-TPO) and anti-thyroglobulin antibodies (Anti-Tg) results were evaluated. All measurements were performed on Cobas c801 (Roche Diagnostics, Penzberg, Germany) using electrochemiluminescence immunoassay technology. Estimated RIs were compared with manufacturer’s by means of RCVs of analytes. ResultsThyroid stimulating hormone values didn’t differ significantly by gender and age. The combined RIs for whole group (N = 28,437) was found as 0.41-4.37 mIU/mL. Free T4 values (11.6-20.1 pmol/L, N = 13,479 in male; 10.5-19.5 pmol/L, N = 17,634 female) and fT3 values (3.38-6.35 pmol/L, N = 2,516 in male; 3.39-5.99 pmol/L, N = 3,348 pmol/L in female) significantly differed by gender (P < 0.050). Both fT4 and fT3 values also showed significant differences in age subgroups comparisons. So, male and female RIs were represented separately for age subgroups. When compared with manufacturer’s RIs, TSH whole group and fT4 subgroups RIs didn’t exceed the analytes’ RCVs, but this difference was greater for fT3. ConclusionsReference interval estimation by use of indirect method out of laboratory data may be more accurate than manufacturer provided RIs. This population based RIs evaluated using RCV of analytes may provide useful information in clinical interpretation of laboratory results

Skeletal muscle energetics in patients with moderate to advanced kidney disease

Sarcopenia, defined as decrease in muscle function and mass, is common in patients with moderate to advanced chronic kidney disease (CKD) and is associated with poor clinical outcomes. Muscle mitochondrial dysfunction is proposed as one of the mechanisms underlying sarcopenia. Patients with moderate to advanced CKD have decreased muscle mitochondrial content and oxidative capacity along with suppressed activity of various mitochondrial enzymes such as mitochondrial electron transport chain complexes and pyruvate dehydrogenase, leading to impaired energy production. Other mitochondrial abnormalities found in this population include defective beta-oxidation of fatty acids and mitochondrial DNA mutations. These changes are noticeable from the early stages of CKD and correlate with severity of the disease. Damage induced by uremic toxins, oxidative stress, and systemic inflammation has been implicated in the development of mitochondrial dysfunction in CKD patients. Given that mitochondrial function is an important determinant of physical activity and performance, its modulation is a potential therapeutic target for sarcopenia in patients with kidney disease. Coenzyme Q, nicotinamide, and cardiolipin-targeted peptides have been tested as therapeutic interventions in early studies. Aerobic exercise, a well-established strategy to improve muscle function and mass in healthy adults, is not as effective in patients with advanced kidney disease. This might be due to reduced expression or impaired activation of peroxisome proliferator-activated receptor-gamma coactivator 1α, the master regulator of mitochondrial biogenesis. Further studies are needed to broaden our understanding of the pathogenesis of mitochondrial dysfunction and to develop mitochondrial-targeted therapies for prevention and treatment of sarcopenia in patients with CKD

The appearance of systemic acquired resistance components in sa-treated or naturally infected Vitis plants by Uncinula necator

WOS: 000245848100023In the present study salicylic acid (SA) treatment and challenge by Uncinula necator (Schwein.) Burr., significantly induced the systemic acquired resistance components in grapevine (Vitis vinifera L. cv. Sultana). Chitinase (CHV, EC 3.2.1.14) and beta-1,3-glucanase (Laminarinase, EC 3.2.1.39) activities increased in SA-treated (100, 200 and 400 ppm) as well as in U. necator infected plants and the highest enzymatic activity was observed in leaves treated with 100 ppm of SA. In contrast, total protein content and accumulation of endogenous free-SA in the leaves were observed at the highest rate of 200 ppm of SA-treatment. Salicylic acid also affected photosynthetic pigments by causing decrease in chlorophyll a and b contents and increase in carotenoids content. Treatment of grapevine leaves with SA provides an attractive tool for the control of powdery mildew disease in grapevine

COMPOUNDS FROM FLOWERS OF Daucus carota L. SSP carota AND THEIR ANTIOXIDANT ACTIVITY

WOS: 00027398100003

The effects of eight weeks of sports activities on self-sufficiency, social skills and some physical fitness parameters in children 9-10 years of age

Cyprus International Conference on Educational Research (CY-ICER) -- FEB 08-11, 2012 -- Middle E Tech Univ No Cyprus Campus, CYPRUSWOS: 000342764800210In this study, the effects of 8 weeks of sports activities on self-sufficiency, social skills and some physical fitness parameters in children 9-10 years of age were examined. The sampling of the study was composed of a total of 45 students with 25 in the test group (10 males and 15 females), and 20 in the control group (8 males and 12 females), attending the Petkim Latife Hanim Primary School in Izmir. Different sports activities (athletics, yoga, basketball, educational games and dancing) were implemented for the test group for a total of 32 class periods. The "General Self-sufficiency Scale", "Matson Evaluation of Social Skills with Youngsters (MESSY)" and the "Physical Fitness Tests" were implemented with the first test, final test method. The SPSS 15.0 program was used in the analysis of the data, the " Paired Samples T-Test" was used in the determination of the differences within the group, whereas, for the independent groups, the " Independent Samples T-Test" was used. It was found from the results obtained that there was not a statistically significant difference in the social skills and self-sufficiency values between the test and control groups (p>0.05). When the change within the test group was considered, it was observed that there was a positive statistically significant difference in the height, weight, flexibility, balance and fat percentages, whereas, it was observed that in the control group there was a positive statistically significant difference only in the height, weight and balance parameters (p<0.05).Cyprus Educ Sci Assoc, Acad World Educ & Res Ctr, Near E Univ, Eastern Mediterranean Univ, Ataturk Teacher Training Acad, Int Cyprus Univ, Girne Amer Univ, Cyprus Educ Sci Asso

IN VITRO SOMATIC EMBRYOGENESIS FROM CORMEL-DERIVED CALLUS CULTURES OF GLADIOLUS ANATOLICUS (BOISS.) STAPF

WOS: 000272422200002Somatic embryogenesis of Gladiolus anatolicus was induced using in vitro obtained cormel segments. Embryogenic callus induction was carried out on Murashige and Skoog (MS) medium containing various concentrations of 2,4-D in dark. The best response was observed in the medium containing 4.5 mu M 2,4-D. Eight weeks after culture initiation, the yellowish-pale and compact calluses were transferred to MS medium containing different concentrations of BA (0.5, 2.2, 4.5, 9.0 and 18.0 mu M) for embryo induction and development. The highest number of somatic embryos (23.6 +/- 3.6) was produced at the lowest concentration of BA (0.5 mu M). The number of somatic embryos was significantly enhanced from 23.6 +/- 3.6 up to 31.6 +/- 3.8 by adding 12% sucrose to the embryonic callus culture media containing 0.5 mu M BA. Somatic embryos in cotyledonarystage were transferred to MS medium supplemented with 0.5 mu M BA and 2% sucrose for maturation and germination. Maximum germination rate was 50%. Application of this protocol has potential in large-scale clonal propagation of the Gladiolus anatolicus for ornamental purposes

In situ Green Synthesis of Cellulose based Silver Nanocomposite and its Catalytic Dye Removal Potential Against Methylene Blue

Pollution of water by leaking industrial dyes has become a major problem for aquatic organisms and human-being. Polluted water treatment methods need efficient alternatives to conventional dye removing techniques. In this study, the catalytic activity of cellulose based silver nanocomposite against the pollutant methylene blue dye was searched. Cellulose based silver nanocomposite was synthesized by using in situ green synthesis method with Laurus nobilis extract. UV-VIS, FTIR, SEM, TEM, EDS, XRD, BET analyses were used to characterization of synthesized nanocomposite. Electron microscopy results confirmed that 25 nm diameter silver nanoparticles were supporting the 25 nm width cellulose nanofibers. Elemental composition of confirmed by UV-VIS, FTIR, XRD and EDS results which including silver and cellulose specific peaks. BET results showed that nanocomposite have 11.1 nm pore size with 199,8508 m(2)/g specific surface area. Catalytic activity of nanocomposite was performed against methylene blue in the presence of NaBH4. Catalytic activity was traced for up to 120 min and complete removal of methylene blue dye was estimated as 1 h. Moreover, the recycled nanocomposite was found reusable without activity loss for three times. These results offer an easy, eco-friendly and reusable dye removing material for wastewaters which were polluted by industrial dyes