Factors affecting the extraction of from Jawasee

Various experimental procedures for the extraction of protease from jawasee powder were adopted to ascertain optimum conditions for leaching- deionized distilled water at pH 7.0 and 37° C was found to extract maximum enzyme in 24 hours. The jawasee powder is optimally active at pH 6.0 with 2.5% egg albumin as a substrate. Sodium chloride at higher concentration inhibits the enzyme considerably. Strong inhibitory action was observed in case of 4 preservatives, namely, para-chloro-meta-cresol, sodium pentachlorophenate, phenyl mercuric nitrate and sodium tri-chlorophenate

Isolation and purification of alhagan, a neutral protease from jawasee

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Fungal lipase and its use in easing sheep skins

The optimum conditions for the maximum hydrolysis of skin lipid by a fungal lipase have been standardised. The skin lipid is found to be optimally hydrolysed at a pH between 5.8-7.9 and at 30°-370°C for one hour. A process for the manufacture of suede clothing leather using this fungal lipase as the decreasing agent has been developed. Using enzymatic pretanning processes viz., unhairing, bating and degreasing, the total load of tannery effluent has been minimised. Comparative chemical, physical and microscopical studies of the quality of leather thus produced from sheep skins showed the good degreasing efficiency of fungal lipase