Histomorphological characteristics of Ascaris lumbricoides and Taeniarhynchus saginatus

Introduction: Ascaridosis and taeniarhynchosis are chronic intestinal parasitic diseases, caused by the multicellular helminthes - Ascaris lumbricoides and Taeniarhynchus saginatus. A. lumbricoides is the largest nematode found in the human intestines with the female organism reaching lengths between 20 and 40 centimeters. T. saginatus is a hermaphroditic cestode reaching lengths from 6 up to 10 meters.Aim: The aim was to prepare histological tissue sections and to present the specific microscopic structure of the intestinal helminthes using different staining techniques.Material and methods: We used museum preparations of adult worms, fixed in a 10% formalin solution. Histological tissue sections were prepared and stained with hemalaun-eosin technique, Heidenhein's azan, van Gieson's trichrome stain and acetic orcein after Taenzer-Unna.Results and discussion: The preparations stained with hemalaun-eosin allowed us to observe and describe the morphology and positioning of the internal organs of the intestinal parasites, while the special stains allowed the differentiation of some specific morphological elements like the fine structure of the cuticle of A. lumbricoides, cestodal eggs' morphology etc.Conclusions: The histological sections allowed a detailed examination of the helminthes' internal morphology. Although in the routine diagnostic practice for intestinal parasites more efficient tests are available, the histological samples stained with different techniques can provide useful information in studying the morphology in the ongoing education of the specialists in human and veterinarian parasitology, biology and laboratory technicians

Demographics of e.vermicularis prevalence in Varna district between 2010 and 2014, evaluated with perianal scotch tape test

Ентеробиозата е най-разпространената в световен мащаб чревна хелминтна инвазия. Целта на това проучване е да се установи екстензитета на опаразитяване с E. vermicularis на базата на амбулаторни паразитологични изследвания сред пациенти от Варненска област. Материал и методи: Проучени са 32 497 изследвания за ентеробиоза, осъществени по метода на скоч теста за 5 годишен период в една от медико-диагностичните лаборатории на гр. Варна. Анализирана е опаразитеността с Е.vermicularis по пол, възраст и населено място.Резултати: Общият екстензитет на опаразитяване е 0,53%, като с по-голяма честота заболяването се диагностира при децата (0,76%)отколкото при възрастните (0,24%). Най-висок е делът на опаразитените от селата (2,64%), следвани от тези, живеещи в по-малките градове на областта (0,72%). С най-малка честота (0,29%) заболяването се регистрира сред пациентите от град Варна.Заключение: Благодарение на лесно осъществимия контактен механизъм на предаване, ентеробиозата остава най-често разпространената хелминтна инвазия при децата и пациентите, живеещи в по-лоши социално битови условия.Enterobiosis is the most common intestinal helminthic infection worldwide. The objective of this study is to determine the prevalence of E.vermicularis infection established with outpatient laboratory tests among the population of Varna region.Material and Methods: The study includes a total number of 32 497 outpatient tests for enterobiosis performed for 5-year period in one of the parasitological laboratories of Varna city. The prevalence of E.vermicularis infection in assessed by age, sex and residence.Results: The total prevalence of the enterobiosis is 0,53%. The prevalence in children (0,76%) is higher than the extensity in the adult (0,24%) population. The highest occurrence was diagnosed among people living in villages (2,64%), followed by those in small towns (0,72%) of the district. The lowest prevalence is detected in Varna city residents (0,29%).Conclusions: Due to the easy `person to person` mode of transmission E.vermicularis remains the most common infection in children and in persons living in rural conditions