32 research outputs found
Shear bond strength and ultrastructural interface analysis of different adhesive systems to Er:YAG laser-prepared dentin
The aim of this study was to evaluate the shear bond strength (SBS) of a microhybrid composite resin bonded with three different adhesive systems to Er:YAG laser- (EL) or bur-prepared dentin surfaces and to analyze the quality and ultrastructure of the adhesive-dentin interfaces by scanning electron microscopy (SEM). The specimens prepared for SBS test and SEM analysis were randomly assigned to eight groups (G1-G8): G1, EL (Fidelis PlusIII, Fotona) + Clearfil S3 Bond (C3S); G2, EL + AdperSE Plus (SE); G3, EL + laser etch + Adper Single Bond2 (SB2); G4, EL + acid etch + SB2; G5, EL + SB2 (no etching); G6, bur + acid etch + SB2; G7, bur + S3; G8, bur + SE. Laser was used in very short pulse mode at a setting of 200 mJ/20 Hz for dentin preparation and at 80 mJ/10 Hz for dentin etching. Bond strength test: 3.5 x 2.0 mm cylindrical molds were placed onto adhesives and filled with the composites. After 24 h in distilled water, SBS was tested at a crosshead speed of 0.5 mm/min. SEM analysis: The dentin-adhesive interfaces were evaluated for the ultrastructure of hybrid layer. Data of SBS (MPa) were statistically analyzed by ANOVA and Tukey HSD. ER:YAG laser-prepared dentin has demonstrated significantly more SBS (p 0.05) in SBS have been determined between the total-etch adhesive applied groups with regard to etching types. SEM analysis revealed that hybrid layers obtained in Er:YAG laser-irradiated dentin exhibited more irregular and non-homogeneous pattern than the conventionally prepared dentin. In conclusion, SE Bond demonstrated superior results in Er:YAG laser-ablated dentin compared to bur-prepared dentin
ASSESSMENT OF THE EFFECT OF ER:YAG LASER CONDITIONING ON BOND STRENGTH OF FISSURE SEALANTS
The purpose of this study was to evaluate the effect of Er:YAG laser enamel etching and/or phosphoric acid enamel etching on shear bond strengths (SBS) of fissure sealants. 42 extracted non-carious third molars, washed and cleaned with a fluoridefree pumice, were sectioned in the cement-enamel junction. Coronal section were embedded in resin and labial surfaces of crowns were flattened for an enamel surface area of 3mm in diameter. The specimens were randomly assigned to six groups of 7 teeth each. Group 1:Acid etching+Clinpro(3M ESPE, USA), Group 2:Er:YAG laser etching (Fidelis Plus II, Fotona)+Clinpro, Group 3:Laser etching+acid etching+Clinpro, Group 4:acid etching+FissuritF(Voco,). Group5:Laser etching+FissuritF, Group 6:Laser etching+acid etching+FissuritF. Samples were etched with 37 % phosphoric acid gel for 20 sec. and/or Er:YAG laser (10 Hz, 120 mJ). Cylindrical molds (D:3mm, H:2mm) were placed on substrate surfaces; and sealants were applied and cylinders of fissure sealants were submitted to SBS test in an Instron machine. SBS data in MPa was statistically analyzed by Tukey Multiple Comparisons test. Mean+ SD values (MPa): Group 1: 14.52 +/- 2.33, Group 2: 5.43 +/- 2.02, Group 3: 9.14 +/- 3,08, Group 4: 9.39 +/- 2.94, Group 5: 5.03 +/- 2.68, Group 6: 8.31 +/- 2.41. Significant differences were determined between Gr 1- Gr 2 (p<0.001), Gr 1- Gr 3 (p<0.01), Gr 1- Gr 4 (p<0.01), Gr 1- Gr 5 (p<0.001), Gr 1- Gr 6 (p<0.01) and Gr 4- Gr 5 (p<0.05). No significant differences were found between the other groups. Laser enamel etching, laser+ acid enamel etching have demonstrated lower SBS values for the fissure sealants tested than enamel acid etching
Oral Bacteria of Children with Turner Syndrome
Aim: Turner syndrome (TS) is a genetic disorder caused by a numerical or structural aberration of the X chromosome, which is associated with a female phenotype. Concerning oral status, several studies have revealed that girls with TS have dental anomalies and periodontal problems. The aim of this study was to evaluate the effects of oral bacteria on caries prevalence and periodontal status in pediatric patients with TS
Assessment of the endodontic microbiota of abscessed primary teeth using microarray technology
Context: Knowledge of the microbial composition of abscessed primary tooth is limited. Aim: The aim was to investigate the presence of 10 oral bacterial species in samples from abscessed primary tooth root canals using microarray technology and to determine their association with clinical findings. Subjects and Methods: The samples were collected from root canals of 20 primary molars with acute primer infection. The bacterial composition of the samples was semi-quantitatively defined using a microarray system (ParoCheck®). Clinical parameters included the presence of spontaneous pain, mobility, percussion sensitivity and swelling. Statistical Analysis: Data were statistically analyzed by Student' t-test, Fisher's exact Chi-square test, Freeman–Halton–Fisher's exact test, and Spearman's rho correlation analysis. Results: All the tested species were detected in the samples. Fusobacterium nucleatum was the most frequent bacterium (100%), followed by Parvimonas micra (65%), Provetella intermedia (45%), and Treponema denticola (45%). According to paired bacterial combinations, F. nucleatum was significantly positively correlated with P. intermedia and P. micra (P < 0.05). T. denticola was significantly positively correlated with Porphyromonas gingivalis, Tannerella forsythia, Campylobacter rectus, and P. micra, while it was negatively correlated with Eikenella corrodens (P < 0.05). No statistically significant relationships were found between the presence of any bacteria and clinical findings. Conclusion: Microarray technology used in this study has demonstrated the presence of various bacteria with varying proportions in the root canals of abscessed primary teeth. The results regarding the high rate of certain bacterial combinations suggest the enhanced pathogenicity due to additive or synergistic effects of these microbial combinations
A novel c.1255G > T (p.D419Y) mutation in SH3BP2 gene causes cherubism in a Turkish family
Cherubism (MIM no. 118400) is a rare autosomal dominant disorder characterized by bilateral multilocular lesions of the upper and lower jaws. The lesions usually manifest clinically during early childhood, progress until puberty, and regress in adulthood. SH3BP2 is the only gene currently known to be associated with cherubism. This study began with an 8-year-old boy who was referred owing to overgrowth of mandible. A panoramic radiograph revealed multilocular radiolucent lesions of the upper/lower jaws, suggestive of cherubism. Sequence analysis of SH3BP2 revealed a novel c.G1255T change in exon 9 of the gene where 80% of the disease-causing mutations were observed. We report here the clinical and molecular findings of a family with 3 affected members in two generations showing variable clinical expressivity with the regression of symptoms with advancing age and the lack of penetrance. (Oral Surg Oral Med Oral Pathol Oral Radiol 2012;114:e42-e46
A Complex Facial Trauma Case with Multiple Mandibular Fractures and Dentoalveolar Injuries
The principles of management of mandibular fractures differ in children when compared to adults and depend on the specific age-related status of the growing mandible and the developing dentition. This paper presents a case report with a complex facial trauma affecting the mandibular body and condyle region and dentoalveolar complex. Clinical examination revealed soft tissue injuries, limited mouth opening, lateral deviation of the mandible, an avulsed incisor, a subluxated incisor, and a fractured crown. CBCT examination revealed a nondisplaced fracture and an oblique greenstick fracture of the mandibular body and unilateral fracture of the condyle. Closed reduction technique was chosen to manage fractures of the mandible. Favorable healing outcomes on multiple fractures of the mandible throughout the 6-year follow-up period proved the success of the conservative treatment. This case report is important since it presents a variety of pathological sequelae to trauma within one case
A Complex Facial Trauma Case with Multiple Mandibular Fractures and Dentoalveolar Injuries
The principles of management of mandibular fractures differ in children when compared to adults and depend on the specific age-related status of the growing mandible and the developing dentition. This paper presents a case report with a complex facial trauma affecting the mandibular body and condyle region and dentoalveolar complex. Clinical examination revealed soft tissue injuries, limited mouth opening, lateral deviation of the mandible, an avulsed incisor, a subluxated incisor, and a fractured crown. CBCT examination revealed a nondisplaced fracture and an oblique greenstick fracture of the mandibular body and unilateral fracture of the condyle. Closed reduction technique was chosen to manage fractures of the mandible. Favorable healing outcomes on multiple fractures of the mandible throughout the 6-year follow-up period proved the success of the conservative treatment. This case report is important since it presents a variety of pathological sequelae to trauma within one case