クルマイス ランナー ノ アンセイ ジ タイシャ リョウ オヨビ エイヨウ セッシュ ジョウキョウ

The purpose of this study was to measure the resting energy expenditure (REE) ofwheelchair athletes and grasp their nutrient intake for the promotion of their health.Seventeen male wheelchair athletes, aged 24-63 years, participated in this research assubjects. The surveys of physical status and food intake frequency by their self-report wereconducted. The measurement of % body fat, mid-arm circumference, and REE was alsotaken. Their BMI and % body fat were almost in the appropriate range, and their mid-armcircumference was bigger than that of Japanese anthropometric reference data. The meanREE was 1,921±438 kcal/day about the subjects aged < 31 yaers (n=6), 1,619±228 kcal/day aged 41-50 years (n=3), 1,699±307 kcal/day aged 51-60 years (n=6), and 1,142 kcal/day aged 61 years (n=2), respectively. The REE and REE/Body weight (BW) weresimilar to each of those about ordinary persons. A positive correlation was observed betweenREE and height as well as BW. The energy and nutrient intakes were rather low,corresponding to each of those at physical activity level I (low) of healthy persons.Considering their appropriate body composition, however, the subjects were not judged in asort of light malnutrition, and their energy intake was considered to meet their requirement.There results suggested that individual nutritional guidance by a well-coordinated system,depending upon the degree of disability, energy expenditure and food intake, is necessary inorder to improve the competence for running and the QOL of wheelchair athletes

Robust detection of undifferentiated iPSC among differentiated cells

Recent progress in human induced pluripotent stem cells (iPSC) technologies suggest that iPSC application in regenerative medicine is a closer reality. Numerous challenges prevent iPSC application in the development of numerous tissues and for the treatment of various diseases. A key concern in therapeutic applications is the safety of the cell products to be transplanted into patients. Here, we present novel method for detecting residual undifferentiated iPSCs amongst directed differentiated cells of all three germ lineages. Marker genes, which are expressed specifically and highly in undifferentiated iPSC, were selected from single cell RNA sequence data to perform robust and sensitive detection of residual undifferentiated cells in differentiated cell products. ESRG (Embryonic Stem Cell Related), CNMD (Chondromodulin), and SFRP2 (Secreted Frizzled Related Protein 2) were well-correlated with the actual amounts of residual undifferentiated cells and could be used to detect residual cells in a highly sensitive manner using qPCR. In addition, such markers could be used to detect residual undifferentiated cells from various differentiated cells, including hepatic cells and pancreatic cells for the endodermal lineage, endothelial cells and mesenchymal cells for the mesodermal lineage, and neural cells for the ectodermal lineage. Our method facilitates robust validation and could enhance the safety of the cell products through the exclusion of undifferentiated iPSC.ISSN:2045-232