Gata6 Promotes GLI3 Repressor Activities in the Limb

Gli3 is a major regulator of Hedgehog signaling during limb development. In the anterior mesenchyme, GLI3 is proteolytically processed into GLI3R, a truncated repressor form that inhibits Hedgehog signaling. Although numerous studies have identified mechanisms that regulate Gli3 function in vitro, it is not completely understood how Gli3 function is regulated in vivo. In this study, we show a novel mechanism of regulation of GLI3R activities in limb buds by Gata6, a member of the GATA transcription factor family. We show that conditional inactivation of Gata6 prior to limb outgrowth by the Tcre deleter causes preaxial polydactyly, the formation of an anterior extra digit, in hindlimbs. A recent study suggested that Gata6 represses Shh transcription in hindlimb buds. However, we found that ectopic Hedgehog signaling precedes ectopic Shh expression. In conjunction, we observed Gata6 and Gli3 genetically interact, and compound heterozygous mutants develop preaxial polydactyly without ectopic Shh expression, indicating an additional prior mechanism to prevent polydactyly. These results support the idea that Gata6 possesses dual roles during limb development: enhancement of Gli3 repressor function to repress Hedgehog signaling in the anterior limb bud, and negative regulation of Shh expression. Our in vitro and in vivo studies identified that GATA6 physically interacts with GLI3R to facilitate nuclear localization of GLI3R and repressor activities of GLI3R. Both the genetic and biochemical data elucidates a novel mechanism by Gata6 to regulate GLI3R activities in the anterior limb progenitor cells to prevent polydactyly and attain proper development of the mammalian autopod

Advanced Laser Diagnostics for Electron Density Measurements

This paper describes innovative laser diagnostics under development at the National Institute for Fusion Science, aiming for the establishment of reliable density measurement techniques in the next step magneticallyconfined fusion devices. There are two approaches, interferometry and polarimetry. A new type of two color laser (47.6/57.2-μm CH3OD) interferometer has been developed and its original function, vibration subtraction,was confirmed in a test stand. The line integrated density measurement at Compact Helical System by using the polarimeter based on Cotton-Mouton effect was demonstrated by the use of a 337-μm HCN laser source

Improvements of CO2 Laser Heterodyne Imaging Interferometer for Electron Density Profile Measurements on LHD

After installation of CO2 laser (wavelength 10.6 μm) heterodyne imaging interferometer (CO2 HI) in 2001, continuous developments have been carried out to improve the measurements capability and stability of operation. The CO2 HI works almost without phase jumping at high electron density (> 1 × 1020 m-3), where the existing far infrared laser (wavelength 118.9 μm) interferometer suffers from fringe jump due to the reduction of signal intensity caused by refraction. However a second interferometer is required to compensate mechanical vibration. A YAG laser (wavelength 1.06 μm) heterodyne imaging interferometer (YAG HI) is presently used for the vibration compensation. In the 10th LHD experimental campaign (2006?2007), sixty four channels of CO2 HI to measure electron density profile and ten channels of YAG HI to measure mechanical vibration are working. A measurement example of a pellet fuelled high-density discharge is reported

Nonrigid chiral soliton for the octet and decuplet baryons

Systematic treatment of the collective rotation of the nonrigid chiral soliton is developed in the SU(3) chiral quark soliton model and applied to the octet and decuplet baryons. The strangeness degrees of freedom are treated by a simplified bound-state approach which omits the locality of the kaon wave function. Then, the flavor rotation is divided into the isospin rotation and the emission and absorption of the kaon. The kaon Hamiltonian is diagonalized by the Hartree approximation. The soliton changes the shape according to the strangeness. The baryons appear as the rotational bands of the combined system of the soliton and the kaon.Comment: 11 pages(LaTex), 1 figures(eps

Calibrations of Fast Ion Flux Measurement Using a Hybrid Directional Probe

A hybrid directional probe method both “thermal and Langmuir probe” was applied for fast ion measure- ments in the compact helical system. In order to obtain absolute values of fast ion density and power density, a calibration of the probe was performed using neutral hydrogen beam and a mixture beam of hydrogen and proton, of which beam current and energy were controlled. The conversion factor from temperature increase of the probe head to local power density and secondary electron emission yield was obtained. The density of fast ions was obtained by directional thermal probe (DTP) method inside the last closed flux surface, and the density ratio was nFastIon/nBulkPlasma = 2.7 × 10?3 at r/a = 0.9. The observation of the directional Langmuir probe (DLP) method is consistent with the DTP results

A Public-key Encryption Scheme Based on Non-linear Indeterminate Equations (Giophantus)

In this paper, we propose a post-quantum public-key encryption scheme whose security depends on a problem arising from a multivariate non-linear indeterminate equation. The security of lattice cryptosystems, which are considered to be the most promising candidate for a post-quantum cryptosystem, is based on the shortest vector problem or the closest vector problem in the discrete linear solution spaces of simultaneous equations. However, several improved attacks for the underlying problems have recently been developed by using approximation methods, which result in requiring longer key sizes. As a scheme to avoid such attacks, we propose a public-key encryption scheme based on the smallest solution problem in the non-linear solution spaces of multivariate indeterminate equations that was developed from the algebraic surface cryptosystem. Since no efficient algorithm to find such a smallest solution is currently known, we introduce a new computational assumption under which proposed scheme is proven to be secure in the sense of IND-CPA. Then, we perform computational experiments based on known attack methods and evaluate that the key size of our scheme under the linear condition. This paper is a revised version of SAC2017

Digital quantitative analysis of mast cell infiltration in interstitial cystitis

AimsTo evaluate the significance of mast cell infiltration in interstitial cystitis (IC) by comparison with equally inflamed controls using a digital quantification technique. MethodsBladder biopsy specimens from 31 patients with Hunner type IC and 38 patients with non-Hunner type IC were analyzed. Bladder biopsy specimens from 37 patients without IC, including 19 non-specific chronic cystitis (non-IC cystitis) specimens and 18 non-inflamed bladder (normal bladder) specimens, were used as controls. Mast cell tryptase-, CD3-, CD20-, and CD138-immunoreactive cells were quantified using digital image analysis software to evaluate both mast cell and lymphoplasmacytic cell densities. Mast cell and lymphoplasmacytic cell densities were counted independently in the entire lamina propria and detrusor areas and compared among the four groups. ResultsIn the lamina propria, there were no significant differences in mast cell and lymphoplasmacytic cell densities between Hunner type IC and non-IC cystitis or between non-Hunner type IC and normal bladder specimens. In the detrusor, the mast cell densities were not significantly different among the four groups. Mast cell density was correlated with lymphoplasmacytic cell density, but not with clinical parameters. ConclusionsMast cell density is not significantly different between IC specimens and non-IC control specimens with a similar degree of background inflammation. The intensity of mast cell infiltration generally correlated with that of lymphoplasmacytic cells. We conclude that mast cell count is of no value in the differential diagnosis between IC and other etiologies

Instability of the hedgehog shape for the octet baryon in the chiral quark soliton model

In this paper the stability of the hedgehog shape of the chiral soliton is studied for the octet baryon with the SU(3) chiral quark soliton model. The strangeness degrees of freedom are treated by a simplified bound-state approach, which omits the locality of the kaon wave function. The mean field approximation for the flavor rotation is applied to the model. The classical soliton changes shape according to the strangeness. The baryon appears as a rotational band of the combined system of the deformed soliton and the kaon.Comment: 24 pages, LaTeX, 8 eps file