5 research outputs found
On the evolutionary emergence of predation
In models for the evolution of predation from initially purely competitive
species interactions, the propensity of predation is most often assumed to be a
direct consequence of the relative morphological and physiological traits of
interacting species. Here we explore a model in which predation ability is an
independently evolving phenotypic feature, so that even when the relative
morphological or physiological traits allow for predation, predation only
occurs if the predation ability of individuals has independently evolved to
high enough values. In addition to delineating the conditions for the
evolutionary emergence of predation, the model reproduces stationary and
non-stationary multilevel food webs with the top predators not necessarily
having size superiority.Comment: 24 pages, 5 figures, typos correcte
The Influence of Copy Number of Targeted Extrachromosomal Genetic Elements on the Outcome of CRISPR-Cas Defense
Prokaryotic type I CRISPR-Cas systems respond to the presence of mobile genetic elements such as plasmids and phages in two different ways. CRISPR interference efficiently destroys foreign DNA harbouring protospacers fully matching CRISPR RNA spacers. In contrast, even a single mismatch between a spacer and a protospacer can render CRISPR interference ineffective but causes primed adaptation - efficient and specific acquisition of additional spacers from foreign DNA into the CRISPR array of the host. It has been proposed that the interference and primed adaptation pathways are mediated by structurally different complexes formed by the effector Cascade complex on matching and mismatched protospacers. Here, we present experimental evidence and present a simple mathematical model that shows that when plasmid copy number maintenance/phage genome replication is taken into account, the two apparently different outcomes of the CRISPR-Cas response can be accounted for by just one kind of effector complex on both targets. The results underscore the importance of consideration of targeted genome biology when considering consequences of CRISPR-Cas systems action