Haematic Potentials of Rhynchospora Corymbosa and Olax Subscorpioïdea Extract in Phenylhydrazine – induced Anaemic Rats

The decoction of the whole plant of Rhynchospora corymbosa and Olax subscorpioïdea leaves are used by the Local traditional healers in the department of Korhogo (Northern Côte d’Ivoire) to treat patients with sickle cell disease. This study was designed to assess, the antianemic potential of these plants extract. Anemia was induced with phenylhydrazine hydrochloride in rats. Animals were divided in normal (N), control (C), test (T), and reference (R) groups. T group included ill-induced Animals treated with R. corymbosa and O. subscorpioidea extracts, and R group ill-induced animals treated with vitamin B9 for anemic animals. Dosing was made as 1-day single dose repeated dose. In anemic animals, the production rate of RBC was significantly (p < 0.001) higher in T group as compared to C group. R. corymbosa whole plant and O. subscorpioidea leaves extracts have a haematic potential. The anti-anaemic effect may partly explain their use in patients with sickle cell disease who are affected by a deficiency of erythrocytes and its components

Haematic Potentials of Rhynchospora Corymbosa and Olax Subscorpioïdea Extract in Phenylhydrazine – induced Anaemic Rats

The decoction of the whole plant of Rhynchospora corymbosa and Olax subscorpioïdea leaves are used by the Local traditional healers in the department of Korhogo (Northern Côte d’Ivoire) to treat patients with sickle cell disease. This study was designed to assess, the antianemic potential of these plants extract. Anemia was induced with phenylhydrazine hydrochloride in rats. Animals were divided in normal (N), control (C), test (T), and reference (R) groups. T group included ill-induced Animals treated with R. corymbosa and O. subscorpioidea extracts, and R group ill-induced animals treated with vitamin B9 for anemic animals. Dosing was made as 1-day single dose repeated dose. In anemic animals, the production rate of RBC was significantly (p < 0.001) higher in T group as compared to C group. R. corymbosa whole plant and O. subscorpioidea leaves extracts have a haematic potential. The anti-anaemic effect may partly explain their use in patients with sickle cell disease who are affected by a deficiency of erythrocytes and its components

Haematic Potentials of Rhyhchospora corymbosa and Olax subscorpioïdea Extracti in Phenylhydrazine-induced Anaemic Rats

The decoction of the whole plant of Rhynchospora corymbosa and Olax subscorpioïdea leaves are used by the Local traditional healers in the department of Korhogo (Northern Côte d’Ivoire) to treat patients with sickle cell disease. This study was designed to assess, the antianemic potential of these plants extract. Anemia was induced with phenylhydrazine hydrochloride in rats. Animals were divided in normal (N), control (C), test (T), and reference (R) groups. T group included ill-induced Animals treated with R. corymbosa and O. subscorpioidea extracts, and R group ill-induced animals treated with vitamin B9 for anemic animals. Dosing was made as 1-day single dose repeated dose. In anemic animals, the production rate of RBC was significantly (p < 0.001) higher in T group as compared to C group. R. corymbosa whole plant and O. subscorpioidea leaves extracts have a haematic potential. The anti-anaemic effect may partly explain their use in patients with sickle cell disease who are affected by a deficiency of erythrocytes and its components

Haematic Potentials of Rhyhchospora corymbosa and Olax subscorpioïdea Extracti in Phenylhydrazine-induced Anaemic Rats

The decoction of the whole plant of Rhynchospora corymbosa and Olax subscorpioïdea leaves are used by the Local traditional healers in the department of Korhogo (Northern Côte d’Ivoire) to treat patients with sickle cell disease. This study was designed to assess, the antianemic potential of these plants extract. Anemia was induced with phenylhydrazine hydrochloride in rats. Animals were divided in normal (N), control (C), test (T), and reference (R) groups. T group included ill-induced Animals treated with R. corymbosa and O. subscorpioidea extracts, and R group ill-induced animals treated with vitamin B9 for anemic animals. Dosing was made as 1-day single dose repeated dose. In anemic animals, the production rate of RBC was significantly (p < 0.001) higher in T group as compared to C group. R. corymbosa whole plant and O. subscorpioidea leaves extracts have a haematic potential. The anti-anaemic effect may partly explain their use in patients with sickle cell disease who are affected by a deficiency of erythrocytes and its components

EVALUATION ET ESSAIS D’OPTIMISATION DE L’ACTIVITE ANTIFONGIQUE DES EXTRAITS DE TERMINALIA MANTALY H.PERRIER, SUR LA CROISSANCE IN VITRO DE ASPERGILLUS FUMIGATUS, CANDIDA ALBICANS, CRYPTOCOCCUS NEOFORMANS ET TRICHOPHYTON MENTAGROPHYTES

Despite the progress being made in medicine, which aims at ensuring best control over the evolution of many previously deadly diseases, the development of medicinal techniques, the proliferation of immunosuppressive treatments and the emergence of new diseases such as HIV / AIDS (responsible for an ever-increasing number of opportunistic fungal infections), the frequency of these fungal infections has unfortunately increased considerably, especially in recent years, due mainly to the increased number of immunocompromised patients. In addition, the mortality rate associated with these fungal infections has become very high. However antifungal drugs especially the most used ones have limitations in their efficacy. So, it is necessary to find new antifungals with improved biological activity. To achieve this, researchers have agreed to come up with new, more effective and less toxic antifungals. So to achieve this goal various approaches have been adopted. Among these, the investigation of the natural environment is very important, necessary and indispensable for the identification and the isolation of new series of molecules or substance with well pronounced biological activity. For this purpose, among the plants selected by our research team is Terminalia mantaly H. Perrier, a combretaceae. This species is well known in the traditional environment especially in southern Africa and Malagasy for its anti-infectious properties. So we decided to investigate these anti-infectious properties and optimize this activity. Thus, we tested different extracts (aqueous, hydro-ethanolic, hexane, acetal and dichloromethanol) obtained either by maceration or by degreasing with Soxhlet from the vegetable powder of Terminalia mantaly. These extracts were tested on the in vitro growth of Candida albicans, Aspergillus fumigatus, Cryptococcus neoformans and Trichophyton mentagrophytes. These fungal isolates are all sensitive to these different extracts in a dose-response relationship. Among these extracts, the T4-2 extract from the degreasing of hexane to Soxhlet is the most active. Of the 18 fractions obtained from Sephadex G25 filtration gel chromatography of the T4-2 extract, only the fractions F8, F9 and F10 grouped in the P fraction have the best antifungal activity: - Aspergillus fumigatus: MFC = 24.37 μg/mL, IC50 = 23 μg/mL -Candida albicans: MFC = 12.1875 μg/mL, IC50 = 1l.23 μg/mL -Cryptococcus neoformans: MFC = 12.1875 μg/mL, IC50 = 5.87 μg/mL -Trichophyton mentagrophytes: MFC = 6.09375 μg/mL, IC50 = 4.75 μg/mL Furthermore, a phytochemical screening coupled with thin layer chromatography and HPLC chromatography revealed that the antifungal activity of the most active fraction P would be due to polyphenols including phenols and Quinones. Therefore, the method used for preparing the T4-2 extract, hexane degreasing of the hydroethanolic extract using soxhlet followed by chromatography on Sephadex G25 filtration gel is a method that not only allows for better concentration but also purify and isolate the active ingredient. We have being able to achieve our goal through this method.Malgré le progrès de la médecine dont l'objectif est de mieux maîtriser l'évolution de nombreuses maladies autrefois mortelles, le développement des techniques médicinales, la multiplication des traitements immunodépresseurs et l'apparition de nouvelles maladies comme le VTH/SIDA (responsables d'un nombre sans cesse croissant de mycoses opportunistes), la fréquence de ces infections fongiques a malheureusement augmentée de façon considérable surtout ces dernières années en raison principalement du nombre accru de patients immunodéprimés. De plus, le taux de mortalité lié à ces infections fongiques est devenu très élevé. Or les médicaments antifongiques surtout les plus utilisés présentent des limites dans leur utilisation. Alors, il s'avère donc nécessaire de trouver de nouveaux antifongiques à activité biologique prononcée. Pour se faire, les chercheurs s'accordent à mettre en place de nouveaux antifongiques plus efficaces et moins toxiques. Ainsi pour atteindre cet objectif diverses approches ont été adoptées. Parmi celles-ci, l'investigation du milieu naturel est d'un capital très important, nécessaire et indispensable pour l’identification et l'isolement de nouvelles têtes de séries de molécules ou de substance à activité biologiques prononcées. A cet effet, parmi les plantes sélectionnées par notre équipe de recherche figure Terminalia mantaly H. Perrier, une combrétacée. Cette espèce est reconnue dans le milieu traditionnel surtout de l'Afrique australe et malgache pour ses propriétés anti-infectieuses. Nous avons donc décidé de vérifier ces vertus infectieuses et d'optimiser cette activité. Ainsi, nous avons testé différents extraits (aqueux, hydro-éthanolique, hexanique, acétatique et dichlorométhanolique) obtenus soit par macération soit par dégraissage au soxhlet à partir de la poudre végétale de Terminalia mantaly. Ces extraits sont testés sur la croissance in vitro de Candida albicans, Aspergillus fumigatus, Cryptococcus neoformans et Trichophyton mentagrophytes. Ces isolats fongiques sont tous sensibles à ces différents extraits selon une relation dose-réponse. Parmi ces extraits, l'extrait T4-2 issu du dégraissage de l’hexane au soxhlet est le plus actif. Des 18 fractions obtenues de la chromatographie sur gel de filtration séphadex G25 de l'extrait T4-2, seules les fractions F8, F9 et F10 regroupées en fraction P présentent la meilleure activité antifongique : - Aspergillus fumigatus : CMF = 24,37 μg/ml CI50 = 23 μg/ml - Candida albicans : CMF = 12,1875 μg/ml CI50 = 1 l, 23 μg/ml - Cryptococcus neoformans : CMF= 12,1875 μg/ml CI50 = 5,87 μg/ml - Trichophyton mentagrophytes : CMF= 6,09375 μg/ml CI50 = 4,75 μg/ml Par ailleurs, un tri phytochimie couplé à des chromatographies sur couche mince et à la chromatographie HPLC révèle que l'activité antifongique de la fraction la plus actives P serait due à des Polyphénols notamment des Phénols et à des Quinones. Ainsi, la méthode de préparation de l'extrait T4-2 par dégraissage à l’hexane au soxhlet de l'extraithydro-éthanolique suivie de chromatographie sur gel de filtration séphadex G25 est une méthode permettant non seulement de mieux concentrer mais aussi de purifier et d'isoler le principe, actif. Par cette méthode, nous avons atteint notre objectif