Chemical Constituent of Acacia auriculiformis Wood Extractives and Their Antioxidant Activity

Acacia auriculiformis showed good potential to be developed as a raw material for bioactive compounds. The heartwood of A. auriculiformis is still rarely explored. The purpose of this study was to identify the bioactive components of the heartwood. Six compounds were identified from the heartwood through nuclear magnetic resonance and mass spectroscopy, namely the C-3,4',7,8 hydroxyl substituted flavonoids and chalcone. Two methylated teracacidins and chalcone-like teracacidin were identified for the first time in the A. auriculiformis heartwood, along with three known compounds. These compounds showed good radical scavenging and reduction activity, compared to crude extract and ascorbic acid, using three different antioxidant assays. The molecular structure-dependent activity was observed to affect the trend of the different antioxidant activities. This finding shows good potential for further development of plant parts of A. auriculiformis from Indonesia as new raw materials for medicines. Keywords: Acacia auriculiformis, antioxidant, chalcone, flavonoid, heartwoo

Metabolite profile, antiproliferative, and antioxidant activity of Acacia auriculiformis and Acacia crassicarpa extracts

Acacia auriculiformis and A. crassicarpa can be used as non-timber forest products to produce therapeutic agents. To achieve this, it is essential to identify the metabolite profile and biological activity of these raw materials. In this study, a liquid chromatography high resolution mass spectrometry platform was used to identify metabolites in the leaf, bark, and heartwood extracts that were positively related to antioxidant and antiproliferative activity using in vitro and in silico studies. A. crassicarpa heartwood extract showed significantly higher antioxidant activity compared to the other extracts. Moreover, the leaf and heartwood extracts from A. auriculiformis, as well as the heartwood extracts from A. crassicarpa, showed significantly high antiproliferative activity. Multivariate analysis using an orthogonal partial least squares model identified several metabolites as significant marker and differentiator compounds between the two Acacia species, with a positive correlation to biological activity. The molecular docking technique predicted various positions of hydroxyl substitution on the flavonoids from A. crassicarpa and A. auriculiformis that alter their binding energy to target receptors, which are related to oxidative stress and cancer treatment targets. Moreover, the methylated flavonoid molecules provide less binding energy. These flavonoids and their derivatives were predominantly found in the heartwood extracts, which demonstrates their potential for further exploration using this part of A. crassicarpa and A. auriculiformis

Antioxidant Activity, Sun Protection Activity, and Phytochemical Profile of Ethanolic Extracts of <i>Daemonorops acehensis</i> Resin and Its Phytosomes

Daemonorops (Indonesian: jernang) resin is one of Indonesia’s leading non-timber forest products and can be developed as a source of natural antioxidants and sun protection. This study aimed to select promising solvents for extracting a Daemonorops acehensis resin and phytosome formulation with high antioxidant capacities and sun protection factor (SPF) values. Jernang resin was extracted using a water–ethanol mixture in five different ratios. The promising extract was then mixed with soy lecithin in three different formulations. A promising extract and phytosome were then selected based on their antioxidant capacities and sun protection factor (SPF) values. A liquid chromatography mass spectrometry/mass spectrometry (LC–MS/MS) analysis was also performed on five extracts to identify the components in the extracts that might be responsible for the biological activity. The results showed that the ethanol solvent variation and phytosome formulation influenced the antioxidant capacity and SPF value. A hundred-percent ethanolic extract and F1 phytosome exhibited the highest antioxidant capacities and SPF values. A qualitative analysis revealed the various classes of compounds in the extract and phytosome. A flavylium chromophore, dracorhodin, dominated the resin extract and was presumed to be the marker compound responsible for their antioxidant capabilities and SPF values. These findings are important for manufacturing sunscreens containing active compounds of bioactive natural resins

Antioxidant Activity, Sun Protection Activity, and Phytochemical Profile of Ethanolic Extracts of Daemonorops acehensis Resin and Its Phytosomes

Daemonorops (Indonesian: jernang) resin is one of Indonesia&rsquo;s leading non-timber forest products and can be developed as a source of natural antioxidants and sun protection. This study aimed to select promising solvents for extracting a Daemonorops acehensis resin and phytosome formulation with high antioxidant capacities and sun protection factor (SPF) values. Jernang resin was extracted using a water&ndash;ethanol mixture in five different ratios. The promising extract was then mixed with soy lecithin in three different formulations. A promising extract and phytosome were then selected based on their antioxidant capacities and sun protection factor (SPF) values. A liquid chromatography mass spectrometry/mass spectrometry (LC&ndash;MS/MS) analysis was also performed on five extracts to identify the components in the extracts that might be responsible for the biological activity. The results showed that the ethanol solvent variation and phytosome formulation influenced the antioxidant capacity and SPF value. A hundred-percent ethanolic extract and F1 phytosome exhibited the highest antioxidant capacities and SPF values. A qualitative analysis revealed the various classes of compounds in the extract and phytosome. A flavylium chromophore, dracorhodin, dominated the resin extract and was presumed to be the marker compound responsible for their antioxidant capabilities and SPF values. These findings are important for manufacturing sunscreens containing active compounds of bioactive natural resins

Exploration of Mango Fruits (Mangifera indica) as α-Glucosidase Inhibitors

Mango fruit (Mangifera indica L.) is the tropical fruit that grows easily in Indonesia with plenty varieties. This study aimed to determine the varieties of mango fruit and the most potent part of mango as antidiabetic agent through α-glucosidase inhibitory activities. Four types of mango fruit (indramayu, manalagi, harum manis, and budiraja) were used in this study. Each part of the mango fruit:peel, flesh, endosperm, and endocarp were extracted by maceration process with three different solvents (n-hexane, ethyl acetate (EtOAc), and ethanol (EtOH)). An ability of all 46 extracts in inhibiting the α-glucosidase at a concentration of 500 ppm were determined. Then 11 extracts with the high inhibition value were determined their IC50 (concentration to inhibit 50% activity) values. EtOAc extract of manalagi, indramayu, and budi raja endosperm had the lowest IC50 value which was not statistically significantly different (at 95%) with EtOAc extract of budi raja peel. The bioautographic Thin Layer Chromatogram showed that the most active band is characterized by white luminescence under UV 366 nm, yellow color under UV 254 and visible light. The band with Rf 0.93 from EtOAc endosperm extract of indramayu and manalagi and Rf 0.73 from EtOAc budi raja peel extract are the most active band which predicted as a flavonoid. The result adds the value of the peel and seed of mango, as well as an alternative in blood sugar control, which is easy to obtain, relatively cheap, and liked by the community. </p

<i>Intsia bijuga</i> Heartwood Extract and Its Phytosome as Tyrosinase Inhibitor, Antioxidant, and Sun Protector

Intsia bijuga (Colebr.) wood (Indonesian: Merbau) is commercial wood with high economic value and is most commonly found in Indonesia. Intsia wood extractives have biological activities related to their potential as natural active ingredients for antiaging cosmetics This study aimed to select the best extraction solvent and phytosome formulation of I. bijuga heartwood extract as an active ingredient for topical antiaging cosmetics. There were five and three variations on extraction solvent and phytosome formulation, respectively. Three main antiaging activity parameters, namely antioxidant, antityrosinase, and sun protection factor (SPF) values, were considered in selecting the best extract and phytosome formula. The results showed that 50% ethanol possessed good antioxidant and antityrosinase activity, but was lower in SPF value, which was significantly different than in other extracts. The phytochemical profile revealed robidanol and robinetin as the main constituent in five I. bijuga extracts. Phytosome F3 possessed high antioxidant, antityrosinase, and SPF values compared to other 50% ethanol phytosome extracts. It could be concluded that I. bijuga ethanol extracts and its phytosome are potent enough to be developed as an antiaging active ingredient in topical use cosmetics

Intsia bijuga Heartwood Extract and Its Phytosome as Tyrosinase Inhibitor, Antioxidant, and Sun Protector

Intsia bijuga (Colebr.) wood (Indonesian: Merbau) is commercial wood with high economic value and is most commonly found in Indonesia. Intsia wood extractives have biological activities related to their potential as natural active ingredients for antiaging cosmetics This study aimed to select the best extraction solvent and phytosome formulation of I. bijuga heartwood extract as an active ingredient for topical antiaging cosmetics. There were five and three variations on extraction solvent and phytosome formulation, respectively. Three main antiaging activity parameters, namely antioxidant, antityrosinase, and sun protection factor (SPF) values, were considered in selecting the best extract and phytosome formula. The results showed that 50% ethanol possessed good antioxidant and antityrosinase activity, but was lower in SPF value, which was significantly different than in other extracts. The phytochemical profile revealed robidanol and robinetin as the main constituent in five I. bijuga extracts. Phytosome F3 possessed high antioxidant, antityrosinase, and SPF values compared to other 50% ethanol phytosome extracts. It could be concluded that I. bijuga ethanol extracts and its phytosome are potent enough to be developed as an antiaging active ingredient in topical use cosmetics