Exploring regulatory fit between service relationships and appeals in co-production

Acknowledgements This work was supported by National Natural Science Foundation of China [grant numbers 72072135; 71872140; 71772141; 71632001] and “the Fundamental Research Funds for the Central Universities” in UIBE [grant number 20QD16].Peer reviewedPostprin

The Rice Pentatricopeptide Repeat Protein PPR756 Is Involved in Pollen Development by Affecting Multiple RNA Editing in Mitochondria.

In land plants, the pentatricopeptide repeat (PPR) proteins form a large family involved in post-transcriptional processing of RNA in mitochondria and chloroplasts, which is critical for plant development and evolutionary adaption. Although studies showed a number of PPR proteins generally influence the editing of organellar genes, few of them were characterized in detail in rice. Here, we report a PLS-E subclass PPR protein in rice, PPR756, loss of function of which led to the abolishment of RNA editing events among three mitochondrial genes includin

A Study on Air Conditioning Thermal Comfort in Building Atrium in Winter

The building atrium is a large space formed by integrating various functions. It runs through multiple floors and is directly or indirectly connected with each floor. In the atrium, people can not only enjoy the air quality of the interior space but also create an open and natural environment characteristic of the outdoor space. In this paper, a winding corridor atrium is the research object, using CFD simulation software to establish a mathematical model. The temperature field, velocity field, and PMV-PPD of the atrium under various working conditions were studied by numerical simulation method, and the thermal comfort of the working area at the bottom of the atrium and the corridor area of each floor were analyzed emphatically. The building atrium is set up in two forms: the upper part is airtight and the upper part is not airtight. Through theoretical analysis and comparison of simulation results, it is concluded that the non-enclosed atrium will help to improve the thermal comfort of the top, and at the same time aggravate the phenomenon of low temperature in the working area at the bottom of the atrium

Genome-Wide Analysis of the Rice Gibberellin Dioxygenases Family Genes

Gibberellins (GAs), a pivotal plant hormone, play fundamental roles in plant development, growth, and stress response. In rice, gibberellin-dioxygenases (GAoxes) are involved in the biosynthesis and deactivation of gibberellins. However, a comprehensive genome-wide analysis of GA oxidases in rice was not uncovered. Here, a total of 80 candidate OsGAox genes were identified and 19 OsGAox genes were further analyzed. Studies on those 19 OsGAox genes, including phylogenetic tree construction, analysis of gene structure, exploration of conserved motifs and expression patterns, were conducted. Results showed that the GAox genes in Arabidopsis and rice were divided into four subgroups and shared some common features. Analysis of gene structure and conserved motifs revealed that splicing phase and motifs were well conserved during the evolution of GAox genes in Arabidopsis and rice, but some special conserved motifs possessed unknown functions need to be further studied. Exploration of expression profiles from RNA-seq data indicated that each GAox gene had tissue-specific expression patterns, although they varied greatly. The expression patterns of these genes under GA3 treatment revealed that some genes, such as OsGA2ox1, OsGA2ox3, OsGA2ox4, OsGA2ox7, OsGA20ox1, and OsGA20ox4, may play a major role in regulating the level of bioactive GA. Taken together, our study provides a comprehensive analysis of the GAox gene family and will facilitate further studies on their roles in rice growth and development so that these genes can be better exploited

Genome-Wide Identification, Expansion Mechanism and Expression Profiling Analysis of GLABROUS1 Enhancer-Binding Protein (GeBP) Gene Family in Gramineae Crops

The GLABROUS1 enhancer-binding protein (GeBP) gene family encodes a typical transcription factor containing a noncanonical Leucine (Leu-)-zipper motif that plays an essential role in regulating plant growth and development, as well as responding to various stresses. However, limited information on the GeBP gene family is available in the case of the Gramineae crops. Here, 125 GeBP genes from nine Gramineae crops species were phylogenetically classified into four clades using bioinformatics analysis. Evolutionary analyses showed that whole genome duplication (WGD) and segmental duplication play important roles in the expansion of the GeBP gene family. The various gene structures and protein motifs revealed that the GeBP genes play diverse functions in plants. In addition, the expression profile analysis of the GeBP genes showed that 13 genes expressed in all tested organs and stages of development in rice, with especially high levels of expression in the leaf, palea, and lemma. Furthermore, the hormone- and metal-induced expression patterns showed that the expression levels of most genes were affected by various biotic stresses, implying that the GeBP genes had an important function in response to various biotic stresses. Furthermore, we confirmed that OsGeBP11 and OsGeBP12 were localized to the nucleus through transient expression in the rice protoplast, indicating that GeBPs function as transcription factors to regulate the expression of downstream genes. This study provides a comprehensive understanding of the origin and evolutionary history of the GeBP genes family in Gramineae, and will be helpful in a further functional characterization of the GeBP genes

Degradation Benefits Polymerization: Photo-Generated Self-Degradable Organo-Catalyst for Higher-Efficiency ATRP and Pure Polymers

In the realm of polymer-based 3D photo printing, challenges arise from the side effects, notably the persistent presence of photocatalyst residues and metal contamination. These impurities pose significant risks in various applications, including electronics, biological tissues, and medical implants. At the same time, spatial-/time-/light-controlled 3D photo printing has been hindered by low-efficiency polymerization concerning both initiation and monomer conversion. To address these criti-cal issues, a pioneering concept, “degradation-inhibited quench,” is introduced and implemented within photopolymeriza-tion to solve the problems mentioned above. This innovative approach aims to produce pure polymers via higher-efficiency Atom Transfer Radical Polymerization (ATRP) with a unique class of diketopyrrolopyrrole (DPP) derivatives as organo-photocatalysts at an extremely low concentration (as low as 50ppm). Through this approach, pure polymers with ultra-high molecular weight (UHMW) have been successfully synthesized. For instance, poly(methyl methacrylate) (PMMA) achieved a monomer conversion of > 50%, a molecular weight of 2.1 million, and a dispersity of 1.38 after 12 h additional dark reaction. Notably, this novel photopolymerization method demonstrates applicability across a broad spectrum of monomers, with or without solvents, including acrylate, acrylic, styrene, and acrylonitrile. Mechanism insights revealed that the production of UHMW PMMA stemmed from the degradation of intermediate complex DPP•+/Br-, which originated from the photo initiation. This degradation inhibited the oxidative quenching of active propagating chain radicals, thereby significantly extending their lifespan. This groundbreaking concept embraces the potential for further development of highly effective organo-photocatalysts and reactive systems specifically tailored for 3D photopolymerization. Moreover, this novel spatial-/time-/light-controlled polymerization approach does not require any additional purification, offering energy and cost-saving manufacturing technology

Analysis of expressed sequence tags from the venom ducts of Conus striatus: focusing on the expression profile of conotoxins

Cone snails (genus Conus) are predatory marine gastropods that use venom peptides for interacting with prey, predators and competitors. A majority of these peptides, generally known as conotoxins demonstrate striking selectivity in targeting specific subtypes of ion channels and neurotransmitter receptors. So they are not only useful tools in neuroscience to characterize receptors and receptor subtypes, but offer great potential in new drug research and development as well. Here, a cDNA library from the venom ducts of a fish-hunting cone snail species, Conus striatus is described for the generation of expressed sequence tags (ESTs). A total of 429 ESTs were grouped into 137 clusters or singletons. Among these sequences, 221 were toxin sequences, accounting for 52.1% (corresponding to 19 clusters) of all transcripts. A-superfamily (132 ESTs) and O-superfamily conotoxins (80 ESTs) constitute the predominant toxin components. Some non-disulfide-rich Conus peptides were also found. The expression profile of conotoxins also explained to some extent the pharmacological and physiological reactions elicited by this typical piscivorous species. For the first time, a nonstop transcript of conotoxin was identified, which is suggestive that alternative polyadenylation may be a means of post-transcriptional regulation of conotoxin production. A comparison analysis of these conotoxins reveals the different variation and divergence patterns in these two superfamilies. Our investigations indicate that focal hyper-mutation, block substitution and exon shuffling are three main mechanisms leading to the conotoxin diversity in a species. The comprehensive set of Conus gene sequences allowed the identification of the representative classes of conotoxins and related components, which may lay the foundation for further research and development of conotoxins