Chemical profiling of Sanjin tablets and exploration of their effective substances and mechanism in the treatment of urinary tract infections

Introduction: Sanjin tablets (SJT) are a well-known Chinese patent drug that have been used to treat urinary tract infections (UTIs) for the last 40 years. The drug consists of five herbs, but only 32 compounds have been identified, which hinders the clarification of its effective substances and mechanism.Methods: The chemical constituents of SJT and their effective substances and functional mechanism involved in the treatment of UTIs were investigated by using high performance liquid chromatography-electrospray ionization-ion trap-time of flight-mass spectrometry (HPLC-ESI-IT-TOF-MSn), network pharmacology, and molecular docking.Results: A total of 196 compounds of SJT (SJT-MS) were identified, and 44 of them were unequivocally identified by comparison with the reference compounds. Among 196 compounds, 13 were potential new compounds and 183 were known compounds. Among the 183 known compounds, 169 were newly discovered constituents of SJT, and 93 compounds were not reported in the five constituent herbs. Through the network pharmacology method, 119 targets related to UTIs of 183 known compounds were predicted, and 20 core targets were screened out. Based on the “compound–target” relationship analysis, 94 compounds were found to act on the 20 core targets and were therefore regarded as potential effective compounds. According to the literature, 27 of the 183 known compounds were found to possess antimicrobial and anti-inflammatory activities and were verified as effective substances, of which 20 were first discovered in SJT. Twelve of the 27 effective substances overlapped with the 94 potential effective compounds and were determined as key effective substances of SJT. The molecular docking results showed that the 12 key effective substances and 10 selected targets of the core targets have good affinity for each other.Discussion: These results provide a solid foundation for understanding the effective substances and mechanism of SJT

Baiji genomes reveal low genetic variability and new insights into secondary aquatic adaptations

The baiji, or Yangtze River dolphin (Lipotes vexillifer), is a flagship species for the conservation of aquatic animals and ecosystems in the Yangtze River of China; however, this species has now been recognized as functionally extinct. Here we report a high-quality draft genome and three re-sequenced genomes of L. vexillifer using Illumina short-read sequencing technology. Comparative genomic analyses reveal that cetaceans have a slow molecular clock and molecular adaptations to their aquatic lifestyle. We also find a significantly lower number of heterozygous single nucleotide polymorphisms in the baiji compared to all other mammalian genomes reported thus far. A reconstruction of the demographic history of the baiji indicates that a bottleneck occurred near the end of the last deglaciation, a time coinciding with a rapid decrease in temperature and the rise of eustatic sea level

Genomic and oncogenic preference of HBV integration in hepatocellular carcinoma

Hepatitis B virus (HBV) can integrate into the human genome, contributing to genomic instability and hepatocarcinogenesis. Here by conducting high-throughput viral integration detection and RNA sequencing, we identify 4,225 HBV integration events in tumour and adjacent non-tumour samples from 426 patients with HCC. We show that HBV is prone to integrate into rare fragile sites and functional genomic regions including CpG islands. We observe a distinct pattern in the preferential sites of HBV integration between tumour and non-tumour tissues. HBV insertional sites are significantly enriched in the proximity of telomeres in tumours. Recurrent HBV target genes are identified with few that overlap. The overall HBV integration frequency is much higher in tumour genomes of males than in females, with a significant enrichment of integration into chromosome 17. Furthermore, a cirrhosis-dependent HBV integration pattern is observed, affecting distinct targeted genes. Our data suggest that HBV integration has a high potential to drive oncogenic transformation

Comparative transcriptomics of 5 high-altitude vertebrates and their low-altitude relatives

Abstract Background: Species living at high altitude are subject to strong selective pressures due to inhospitable environments (e.g., hypoxia, low temperature, high solar radiation, and lack of biological production), making these species valuable models for comparative analyses of local adaptation. Studies that have examined high-altitude adaptation have identified a vast array of rapidly evolving genes that characterize the dramatic phenotypic changes in high-altitude animals. However, how high-altitude environment shapes gene expression programs remains largely unknown. Findings: We generated a total of 910 Gb of high-quality RNA-seq data for 180 samples derived from 6 tissues of 5 agriculturally important high-altitude vertebrates (Tibetan chicken, Tibetan pig, Tibetan sheep, Tibetan goat, and yak) and their cross-fertile relatives living in geographically neighboring low-altitude regions. Of these, ∼75% reads could be aligned to their respective reference genomes, and on average ∼60% of annotated protein coding genes in each organism showed FPKM expression values greater than 0.5. We observed a general concordance in topological relationships between the nucleotide alignments and gene expression–based trees. Tissue and species accounted for markedly more variance than altitude based on either the expression or the alternative splicing patterns. Cross-species clustering analyses showed a tissue-dominated pattern of gene expression and a species-dominated pattern for alternative splicing. We also identified numerous differentially expressed genes that could potentially be involved in phenotypic divergence shaped by high-altitude adaptation. Conclusions: These data serve as a valuable resource for examining the convergence and divergence of gene expression changes between species as they adapt or acclimatize to high-altitude environments

Urban Resilience for Urban Sustainability: Concepts, Dimensions, and Perspectives

Urbanization is a continuous process for a city’s economic development. Though rapid urbanization provides a huge employment opportunity for people, urban threats also increase proportionately due to natural and man-made hazards. Understanding urban resilience and sustainability is an urgent matter to face hazards in the rapidly urbanized world. Therefore, this study aims to clarify the concept and develop key indications of urban resilience and sustainability from the existing literature. A systematic literature review guided by PRISMA has been conducted using literature from 1 January 2001 to 30 November 2021. It argues that sustainability and resilience are interrelated paradigms that emphasize a system’s capacity to move toward desirable development paths. Resilience and sustainability are fundamentally concerned with preserving societal health and well-being within the context of a broader framework of environmental change. There are significant differences in their emphasis and time scales, particularly in the context of urbanization. This study has identified key indicators of urban resilience under three major components like adaptive capacity (education, health, food, and water), absorptive capacity (community support, urban green space, protective infrastructure, access to transport), and transformative capacity (communication technology, collaboration of multi-stakeholders, emergency services of government, community-oriented urban planning). This study also identified several indicators under major dimensions (social, economic, and environmental) of urban sustainability. The findings will be fruitful in understanding the dynamics of urban vulnerability and resilience and its measurement and management strategy from developed indicators

Assessment of Critical Diffusion Factors of Public–Private Partnership and Social Policy: Evidence from Mainland Prefecture-Level Cities in China

Cooperation between government and social capital is an important starting point in the supply-side reform of public services. It is also an effective practice in public governance innovation. Based on policy diffusion theory and event history analysis (EHA), this study analyzes panel data from 282 mainland prefecture-level cities in China from 2004–2020 to explore public–private partnerships’ critical diffusion factors. The study reveals that motivation factors, resource/obstacle factors, and external factors affect government and social capital cooperation policies to different extents. The main driving forces for local governments to adopt these policies are population size, level of economic development, government financial resources, the learning mechanism, and the imitation mechanism. This study proposes the following arguments: firstly, that the ultimate goal of policy innovation is to solve social contradictions and meet public demand; secondly, that economic resources can help to adopt policy innovation and proper diffusion; thirdly, that the public–private partnership (PPP) model has been continuously developed by using experience from other projects or cities through a learning mechanism; and finally, that policy publicity and public opinion expressed via the mainstream media are not only an inducement for policy innovation and diffusion, but also a powerful guarantee. The experience of local governments in China can help to verify whether the “positive factors” that are traditionally considered to be conducive to the cooperation between the government and social capital are effective, and to reveal the internal logic of the innovation diffusion of public policies of local governments in China from a more multidimensional perspective