First confirmed report of Nassarius sinarum (Mollusca, Gastropoda) in Korea

The marine gastropod mollusc Nassarius sinarum has attracted attention due to its status as a potential invasive species and the ecological impact it may have on local environments and the fishing industry. It was observed exclusively within China initially, but its distribution now seems to have expanded into Japan and Korea. Accurate identification of N. sinarum, particularly in its juvenile stage, is vital for understanding its ecological influences and distribution patterns.This study represents the first comprehensive analysis of N. sinarum samples from Korea. It includes morphological examination, scanning electron microscopy images and molecular sequencing. Two live specimens were collected from the Yeongsan River estuary in Korea and their morphological features were analysed and compared to those of samples from China and Japan. The samples’ species were confirmed by molecular identification, based on cytochrome c oxidase subunit I (COI) and histone H3 (H3) genetic markers.It was observed that juvenile N. sinarum shells lack key species-characteristic morphological traits, such as a thick outer lip and diminishing axial ribs. However, COI marker-based molecular identification affirmed that these Korean specimens were N. sinarum. The H3 region was registered with the National Center for Biotechnology Information (NCBI) for the first time. Phylogenetic analysis of the H3 region did not resolve species distinctions within the Nassarius, suggesting that the H3 marker is not suitable for species identification within this genus. In this context, multiple genetic markers, when used appropriately, can also be applied to genus-level searches, enhancing species identification accuracy and reducing misidentification.The sequences provided in this study can serve as a valuable reference for future DNA barcoding research. Additional samples and surveys should be conducted through collaborative efforts amongst national and institutional organisations to further clarify the ecological status of N. sinarum and to investigate its distribution and potential impact around East Asia. Finally, a new Korean name, (No-lan-jul-job-ssal-mu-nui-go-dung; 노란줄좁쌀무늬고둥) has been proposed for N. sinarum

Chemoenzymatic Synthesis of Glycosylated Macrolactam Analogues of the Macrolide Antibiotic YC‐17

YC‐17 is a 12‐membered ring macrolide antibiotic produced from Streptomyces venezuelae ATCC 15439 and is composed of the polyketide macrolactone 10‐deoxymethynolide appended with D‐desosamine. In order to develop structurally diverse macrolactam analogues of YC‐17 with improved therapeutic potential, a combined approach involving chemical synthesis and engineered cell‐based biotransformation was employed. Eight new antibacterial macrolactam analogues of YC‐17 were generated by supplying a novel chemically synthesized macrolactam aglycone to S. venezuelae mutants harboring plasmids capable of synthesizing several unnatural sugars for subsequent glycosylation. Some YC‐17 macrolactam analogues were active against erythromycin‐resistant bacterial pathogens and displayed improved metabolic stability in vitro. The enhanced therapeutic potential demonstrated by these glycosylated macrolactam analogues reveals the unique potential of chemoenzymatic synthesis in antibiotic drug discovery and development.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/113147/1/adsc_201500250_sm_miscellaneous_information.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/113147/2/2697_ftp.pd

Kaposin-B Enhances the PROX1 mRNA Stability during Lymphatic Reprogramming of Vascular Endothelial Cells by Kaposi's Sarcoma Herpes Virus

Kaposi's sarcoma (KS) is the most common cancer among HIV-positive patients. Histogenetic origin of KS has long been elusive due to a mixed expression of both blood and lymphatic endothelial markers in KS tumor cells. However, we and others discovered that Kaposi's sarcoma herpes virus (KSHV) induces lymphatic reprogramming of blood vascular endothelial cells by upregulating PROX1, which functions as the master regulator for lymphatic endothelial differentiation. Here, we demonstrate that the KSHV latent gene kaposin-B enhances the PROX1 mRNA stability and plays an important role in KSHV-mediated PROX1 upregulation. We found that PROX1 mRNA contains a canonical AU-rich element (ARE) in its 3′-untranslated region that promotes PROX1 mRNA turnover and that kaposin-B stimulates cytoplasmic accumulation of the ARE-binding protein HuR through activation of the p38/MK2 pathway. Moreover, HuR binds to and stabilizes PROX1 mRNA through its ARE and is necessary for KSHV-mediated PROX1 mRNA stabilization. Together, our study demonstrates that kaposin-B plays a key role in PROX1 upregulation during lymphatic reprogramming of blood vascular endothelial cells by KSHV

Optimization of operation times of a heating system in office building

A method is proposed for optimizing the operation times of a heating system in an office building for saving energy. The method involves determining the optimal start and stop times of the heating system by using an optimized artificial neural network (ANN) model, which was developed in this study. A program based on back-propagation learning was used for ANN learning. Furthermore, the amount of initial learning data, the optimal time interval for measuring the input data and the acceptable error for the practical application of the ANN model to real buildings were determined from the results of a daily simulation performed using the optimized ANN model integrated with a program for room air temperature prediction. An evaluation of the ANN’s performance in determining the optimal start and stop times of a building heating system for unexperienced learning data showed its potential to save energy

First reliable report of the Nassarius sinarum (Mollusca, Gastropoda) from Korea as an invasive species

Two live specimens of the mud snail, Nassarius sinarum, was collected from the Yeongsan River estuary in Jeollanam-do, Korea, during June 2022. The species was identified based on its morphology and DNA barcoding.Morphological descriptions and photographs of N. sinarum were used to verify its occurrence in Korean waters as an invasive species. The species is characteristic in its axial ribs, which weaken towards the outer lip. Molecular analysis of the mitochondrial DNA cytochrome oxidase subunit I gene showed that the specimen belonged to N. sinarum with 98.48–99.69% sequence similarity. The new Korean name of “No-lan-jul-job-ssal-mu-nui-go-dung; 노란줄좁쌀무늬고둥” was proposed for the species

First reliable report of the Nassarius sinarum (Mollusca, Gastropoda) from Korea as an invasive species

Two live specimens of the mud snail, Nassarius sinarum, was collected from the Yeongsan River estuary in Jeollanam-do, Korea, during June 2022. The species was identified based on its morphology and DNA barcoding.Morphological descriptions and photographs of N. sinarum were used to verify its occurrence in Korean waters as an invasive species. The species is characteristic in its axial ribs, which weaken towards the outer lip. Molecular analysis of the mitochondrial DNA cytochrome oxidase subunit I gene showed that the specimen belonged to N. sinarum with 98.48–99.69% sequence similarity. The new Korean name of “No-lan-jul-job-ssal-mu-nui-go-dung; 노란줄좁쌀무늬고둥” was proposed for the species