Th17 i IL-17 osiągają wyższe stężenia w przebiegu martwicy głowy kości udowej i są dodatnio skorelowane z nasileniem bólu

Objective: Synovitis associated with osteonecrosis of the femoral head (ONFH) is responsible for several clinical symptoms. However, the mechanisms underlying synovitis and the inflammatory environment remain unclear. This study analyzed the proinflammatory mediation expression of IL-17 and Th17, which perform key functions in regulating inflammatory processes in the inflamed synovium and peripheral blood in ONFH. Methods: Synovial fluid from the hips of 23 patients and 5 controls was collected during surgery, and peripheral blood samples were obtained from 34 patients and 9 controls. The expression of IL-17 in the synovium was detected by immunohistochemistry, and the levels of Th17 and IL-17 in the blood were measured by flow cytometry and ELISA. Pain assessment was performed for all the patients and controls. Results: An inflamed synovium was characterized by increased leukocyte infiltration and IL-17 expression in comparison with the control. Preoperative levels of Th17 and IL-17 were significantly higher in the peripheral blood of the ONFH group than those in the controls. The symptoms were also positively correlated with the Th17 levels of the ONFH patients. Conclusion: Th17 cells were recruited to an inflamed synovium, and inflammatory cytokine IL-17 was expressed at an increased level in the hip synovium of ONFH patients, which possibly contributed to clinical syndrome development. Overall, this study will help in identifying new therapeutic strategies for ONFH, especially the targeting of IL-17 to decrease inflammation and pain. Wstęp: Zapalenie błony maziowej związane z martwicą głowy kości udowej (osteonecrosis of the femoral head; ONFH) odpowiada za kilka objawów klinicznych, jednak mechanizmy leżące u podstaw zapalenia błony maziowej oraz środowisko zapalne pozostają niejasne. W niniejszym badaniu poddano analizie ekspresję mediatora zapalenia IL-17 na limfocytach Th17, które pełnią kluczową rolę w regulowaniu procesów zapalnych w objętej stanem zapalnym błonie maziowej i krwi obwodowej w przebiegu ONFH. Materiał i metody: Podczas zabiegów operacyjnych pobrano maź stawową ze stawów biodrowych 23 pacjentów i 5 osób z grupy kon­trolnej, natomiast próbki krwi obwodowej uzyskano od 34 pacjentów i 9 osób z grupy kontrolnej. Ekspresję IL-17 w błonie maziowej wykrywano za pomocą metody immunohistochemicznej, a stężenie Th17 i IL-17 we krwi mierzono metodą cytometrii przepływowej i metodą ELISA. U wszystkich pacjentów i osób z grupy kontrolnej oceniono parametr bólu. Wyniki: Cechą charakterystyczną objętej stanem zapalnym błony maziowej był wzrost nacieczenia limfocytarnego i ekspresji IL-17 w porównaniu z grupą kontrolną. Stężenie Th17 i IL-17 przed wykonaniem zabiegów operacyjnych było znacząco wyższe we krwi obwodowej pacjentów z martwicą głowy kości udowej niż grupy kontrolnej. Również objawy były u tych pacjentów dodatnio skorelowane z poziomem Th17. Wnioski: Limfocyty Th17 były rekrutowane do objętej stanem zapalnym błony maziowej, a cytokina zapalna IL-17 ulegała ekspresji na zwiększonym poziomie w błonie maziowej stawu biodrowego pacjentów z martwicą głowy kości udowej, co prawdopodobnie przyczyniło się do rozwoju zespołu objawów klinicznych. Uogólniając, niniejsze badanie może pomóc zidentyfikować nowe strategie terapeutyczne w martwicy głowy kości udowej, w szczególności ukierunkowane na IL-17 w celu zmniejszenia stanu zapalnego i bólu

A Probe into Cultivation Path of Students’ Ability in Innovative Digital Technology Design Based on Design Thinking

Having risen to the height of the future development of the country, the cultivation of innovative digital technology design talents has become the general trend of the development of the education industry of the world. How to cultivate students’ innovative ability is a huge challenge for the current education circle. Design thinking is a creative problem-solving thinking model and methodology separated from design. The Paper started with the essence of design thinking. The application and practice of all institutions on the innovation process of design thinking were analyzed, and its modes and ideas in the fields of industry, commerce, and design were combined. The innovative curriculum design was carried out based on the five foundations of the design thinking model of Stanford University, and the operability of teaching links, the iterative cycle of the process, and the effectiveness of students’ hands-on actions were emphasized. In the Paper, effective operation guidance for the cultivation of students’ innovation ability was provided. Besides, a cyclic and iterative co-creation ecosystem in colleges and universities can be built to link the integration of industry and education with digital technology design innovation. In the Paper, the “three innovations” (creativity, innovation, entrepreneurship) were focused to explore a new path to cultivate students’ ability in innovative digital technology design, which is of great significance to the cultivation of innovative talents in digital technology design, the reform of teaching forms, and teaching contents

Strategies for manipulating Rubisco and creating photorespiratory bypass to boost C3 photosynthesis : Prospects on modern crop improvement

Photosynthesis is a process that uses solar energy to fix CO2 in the air and converts it into sugar, and ultimately powers almost all life activities on the earth. C3 photosynthesis is the most common form of photosynthesis in crops. Current efforts of increasing crop yields in response to growing global food requirement are mostly focused on improving C3 photosynthesis. In this review, we summarized the strategies of C3 photosynthesis improvement in terms of Rubisco properties and photorespiratory limitation. Potential engineered targets include Rubisco subunits and their catalytic sites, Rubisco assembly chaperones, and Rubisco activase. In addition, we reviewed multiple photorespiratory bypasses built by strategies of synthetic biology to reduce the release of CO2 and ammonia and minimize energy consumption by photorespiration. The potential strategies are suggested to enhance C3 photosynthesis and boost crop production

Improvement of phytoremediation potential of Indian mustard by sulfur treatment of cadmium-contaminated soil

The phytoremediation potential of Indian mustard (Brassica juncea) on the cadmium-contaminated soil was investigated under the treatment of sulfur (15, 30 and 60 g/kg soil). The effects of the sulfur treatment were evaluated by measuring the biomass and root vitality of the plants, enzymatic activities, and the content of malondialdehyde and Cd. The results show that the biomass and root vitality of the plants were significantly increased, and the activities of superoxide dismutase and catalase were improved when the soil was treated with 30 g sulfur /kg soil, while the activities of peroxidase and malondialdehyde were decreased. The total Cd in the plants treated with 15 g/kg soil was 2.8 times higher than that in the control plants. In summary, the results indicate that the addition of sulfur could promote the growth of Indian mustard and promote the uptake of Cd. As such, the treatment of cadmium-contaminated soil with sulfur can be used as a strategy for the removal of cadmium contamination by improving the phytoremediation potential of Indian mustard

Arsenic Adsorption and Desorption in Various Aqueous Media in the Nearshore Zone and Influencing Factors

A profound understanding of the adsorption and desorption characteristics of arsenic on various media in aqueous solutions is helpful for evaluating the behavior of arsenic in groundwater. In this study, the characteristics of arsenic adsorption and desorption on aqueous media including silty clay, fine sand, medium sand, and coarse sand with gravel from Shenyang Huangjia water source, China were investigated by batch experiments. The results showed that the aqueous media in the study area had a strong fixation ability for arsenic, and both physical and chemical adsorption of arsenic occurred. Among them, silty clay had the strongest adsorption capacity and the largest buffer capacity for arsenic. As the specific surface area (SSA) of the medium decreased, the adsorption capacity decreased, and the desorption capacity increased. There was an obvious positive correlation between the desorption capacity and adsorption capacity of arsenic, and the force of the adsorption process was larger than that of the desorption process. The pH, temperature, carbonate, and ionic strength all affected the arsenic adsorption and desorption, and adsorption of arsenic occurred mainly by obligate adsorption in the study area

IN VIVO MOTION OF FEMORAL CONDYLES DURING WEIGHT-BEARING FLEXION AFTER ANTERIOR CRUCIATE LIGAMENT RUPTURE USING BIPLANE RADIOGRAPHY

The purpose of this study was to investigate in vivo three- dimensional tibiofemoral kinematics and femoral condylar motion in knees with anterior cruciate ligament (ACL) deficiency during a knee bend activity. Ten patients with unilateral ACL rupture were enrolled. Both the injured and contralateral normal knees were imaged using biplane radiography at extension and at 15°, 30°, 60°, 90°, and 120° of flexion. Bilateral knees were next scanned by computed tomography, from which bilateral three-dimensional knee models were created. The in vivo tibiofemoral motion at each flexion position was reproduced through image registration using the knee models and biplane radiographs. A joint coordinate system containing the geometric center axis of the femur was used to measure the tibiofemoral motion. In ACL deficiency, the lateral femoral condyle was located significantly more posteriorly at extension and at 15° (p < 0.05), whereas the medial condylar position was changed only slightly. This constituted greater posterior translation and external rotation of the femur relative to the tibia at extension and at 15° (p < 0.05). Furthermore, ACL deficiency led to a significantly reduced extent of posterior movement of the lateral condyle during flexion from 15° to 60° (p < 0.05). Coupled with an insignificant change in the motion of the medial condyle, the femur moved less posteriorly with reduced extent of external rotation during flexion from 15° to 60° in ACL deficiency (p < 0.05). The medial- lateral and proximal-distal translations of the medial and lateral condyles and the femoral adduction-abduction rotation were insignificantly changed after ACL deficiency. The results demonstrated that ACL deficiency primarily changed the anterior-posterior motion of the lateral condyle, producing not only posterior subluxation at low flexion positions but also reduced extent of posterior movement during flexion from 15° to 60

Insights into the Fungal Community and Functional Roles of Pepper Rhizosphere Soil under Plastic Shed Cultivation

The rhizosphere fungal community is essential for determining plant health and improving crop productivity. The fungal community structure and functional roles in the plastic shed soils were explored using high throughput sequencing and FUNGuild in this study. The fungal community structures shifted between the rhizosphere and non-rhizosphere soils. The greatest abundance variation was observed for the rare fungal members with relative abundances &lt;0.1%. In the rhizosphere soil of pepper, the abundance of the genera Purpureocillium, Metacorgyceps, Arthrobotrys, Cephalotheca, and Scedosporium increased significantly, among which, Purpureocillium, Arthrobotrys and Metacorgyceps exhibited biocontrol characteristics. Co-occurrence network analysis revealed different interactions of fungal communities in the rhizosphere and non-rhizosphere soils, both of which were dominated by low abundance members. More positive correlation was identified among the rare members, the fungal pathotroph functions and phthalate acid ester in the rhizosphere soil. This study highlights the important niche of the rare fungal members in soil microbial ecology under plastic shed cultivation