Investigating Comprehension Differences Between Active and Passive Sentences in a Young and Older Adult Population

The current investigation sought to determine whether normal, non-impaired young and older adults performed differentially on the active and passive sentences of the Computerized Revised Token Test - Active/Passive (CRTT-A/P). Twenty-five young adults (18 to 30 years old) and twenty-five older adults (50 to 80 years old) completed this study.The first purpose of this study was to determine if the two groups (i.e., young and older) differed significantly in their performance (as measured by overall mean scores) on the two sentence types (i.e., active and passive sentences) across each of the four subtests. Significant differences between subtests were found; participants obtained significantly higher scores on Subtest VIII than on Subtest V and Subtest VI.The second purpose of this study was to determine if the two groups differed significantly in their efficiency scores on the two sentence types across each of the four subtests. It was found that (1) participants obtained significantly higher efficiency scores on Subtest VII than on Subtests V and VI and that (2) participants obtained significantly higher efficiency scores on Subtest VIII than on Subtest VI.The final purpose of this study was to determine if the two groups differed significantly in their response times on the two sentence types across each of the four subtests. It was found that: (1) participants responded more quickly to the passive sentence type than to the active sentence type across all four subtests; (2) the older group responded more quickly on Subtest V than on Subtests VI and VIII; and (3) the older group responded more quickly on Subtest VII than on Subtests VI and VIII.When differences between the two groups were examined for all of the measures, only one significant difference was found (the older group performed significantly slower than the young group on Subtests VI and VIII). These findings demonstrate that language comprehension abilities remain relatively constant with age. While the CRTT-A/P did not detect any significant comprehension differences between active and passive sentences in young and older individuals, this test has yet to be administered to various pathological groups (e.g., persons with agrammatic comprehension). This study thus provides preliminary data for future comparisons with pathological populations. Such studies are equally important for advancing our understanding of the way we comprehend language and form grammatical meaning

Coping with Coping:International migrants’ experiences of the Covid-19 lockdown in the UK

Globally, policymakers have overlooked the challenges faced by international migrants in host countries during the Covid-19 pandemic. The policies and support systems designed by host governments highlight the lack of social justice and raise concerns for scholarly attention. Considering the experiences of international migrants living in the UK during the Covid-19 lockdown from the theoretical perspective of coping, this interpretivist study investigates international migrants’ coping strategies adopted during the first UK national lockdown. Data collected from 60 Chinese, Italian and Iranian migrants using semi-structured interviews during the lockdown period were analysed thematically using NVivo. The findings show that migrants adopted multi-layered and multi-phase coping strategies. To cope with the anxiety and uncertainties caused by the pandemic, they initiated new practices informed by both home and host institution logics. Nevertheless, the hostile context's responses provoked unexpected new worries and triggered the adoption of additional and compromising practices. The paper illustrates how coping became paradoxical because migrants had to cope with the hostile reactions that their initial coping strategies provoked in the host environment. By introducing the new concept of coping with coping, this paper extends previous theoretical debate and leads to several managerial implications for governments and policymakers.</p

Personalized Estimate of Chemotherapy-Induced Nausea and Vomiting: Development and External Validation of a Nomogram in Cancer Patients Receiving Highly/Moderately Emetogenic Chemotherapy.

Chemotherapy-induced nausea and vomiting (CINV) is presented in over 30% of cancer patients receiving highly/moderately emetogenic chemotherapy (HEC/MEC). The currently recommended antiemetic therapy is merely based on the emetogenic level of chemotherapy, regardless of patient's individual risk factors. It is, therefore, critical to develop an approach for personalized management of CINV in the era of precision medicine.A number of variables were involved in the development of CINV. In the present study, we pooled the data from 2 multi-institutional investigations of CINV due to HEC/MEC treatment in Asian countries. Demographic and clinical variables of 881 patients were prospectively collected as defined previously, and 862 of them had full documentation of variables of interest. The data of 548 patients from Chinese institutions were used to identify variables associated with CINV using multivariate logistic regression model, and then construct a personalized prediction model of nomogram; while the remaining 314 patients out of China (Singapore, South Korea, and Taiwan) entered the external validation set. C-index was used to measure the discrimination ability of the model.The predictors in the final model included sex, age, alcohol consumption, history of vomiting pregnancy, history of motion sickness, body surface area, emetogenicity of chemotherapy, and antiemetic regimens. The C-index was 0.67 (95% CI, 0.62-0.72) for the training set and 0.65 (95% CI, 0.58-0.72) for the validation set. The C-index was higher than that of any single predictor, including the emetogenic level of chemotherapy according to current antiemetic guidelines. Calibration curves showed good agreement between prediction and actual occurrence of CINV.This easy-to-use prediction model was based on chemotherapeutic regimens as well as patient's individual risk factors. The prediction accuracy of CINV occurrence in this nomogram was well validated by an independent data set. It could facilitate the assessment of individual risk, and thus improve the personalized management of CINV

Determination of protein–DNA binding constants and specificities from statistical analyses of single molecules: MutS–DNA interactions

Atomic force microscopy (AFM) is a powerful technique for examining the conformations of protein–DNA complexes and determining the stoichiometries and affinities of protein–protein complexes. We extend the capabilities of AFM to the determination of protein–DNA binding constants and specificities. The distribution of positions of the protein on the DNA fragments provides a direct measure of specificity and requires no knowledge of the absolute binding constants. The fractional occupancies of the protein at a given position in conjunction with the protein and DNA concentrations permit the determination of the absolute binding constants. We present the theoretical basis for this analysis and demonstrate its utility by characterizing the interaction of MutS with DNA fragments containing either no mismatch or a single mismatch. We show that MutS has significantly higher specificities for mismatches than was previously suggested from bulk studies and that the apparent low specificities are the result of high affinity binding to DNA ends. These results resolve the puzzle of the apparent low binding specificity of MutS with the expected high repair specificities. In conclusion, from a single set of AFM experiments, it is possible to determine the binding affinity, specificity and stoichiometry, as well as the conformational properties of the protein–DNA complexes

Proteolytic Processing of Rubella Virus Nonstructural Proteins

AbstractThe genomic RNA of rubella virus contains two long open reading frames (ORF), a 5′-proximal ORF that codes for the nonstructural proteins and a 3′-proximal ORF that encodes the structural proteins. The cDNA encoding the nonstructural protein ORF of the wild-type M33 strain of rubella virus has been obtained and sequenced. Comparison between the nonstructural proteins of the M33 and Therien strains of rubella virus revealed a 98% homology in nucleotide sequence and 98.1% in deduced amino acid sequence. To examine the processing of rubella virus nonstructural protein, the complete nonstructural protein ORF was expressed in BHK cells using a pSFV expression vector. Three nonstructural protein products (p200, p150, and p90) with molecular weights of 200, 150, and 90 kDa were identified using antisera raised against synthetic peptides corresponding to regions of the nonstructural proteins. p200 is the polyprotein precursor, while p150 and p90 are the cleavage products. Site-directed mutagenesis of the Cys-1151 residue (one of the catalytic dyad residues of the viral protease) and of the Gly-1300 residue (the viral protease cleavage site) abrogated protease activity and p200 precursor cleavage, respectively. Coexpression of mutant constructs in BHK cells indicated that rubella virus protease can function both in cis and in trans

The role of school connectedness and friend contact in adolescent loneliness, and implications for physical health

JI was supported by the Medical Research Council (MC_UU_00022/1) and the Scottish Government Chief Scientist Office (SPHSU16). The HBSC 2013/14 study in Scotland was funded by NHS Health Scotland (now Public Health Scotland).The current study investigated how adolescents' loneliness relates to school connectedness, classmate support, teacher support, and offline and online communication with friends. We also examined the association between loneliness, physical health, and sleep. Data came from the Scottish Health Behavior in School-aged Children (HBSC). The total sample was 2983 adolescents (F = 1479 [49.6%]) aged 14-17 years (M = 15.66, SD = 0.39) from 117 secondary schools in Scotland. Results showed that (1) higher teacher support, classmate support, and offline contact with friends predicted lower levels of loneliness, (2) online friendship engagement predicted higher levels of loneliness, and (3) poor health and sleep were positively associated with loneliness. The study offers new findings, highlighting the role played by classmates/peers and teachers in reducing loneliness. Supporting previous research, we also found associations between loneliness, poor sleep, and worse physical health.Publisher PDFPeer reviewe

Simulating the impact of greenspace exposure on metabolic biomarkers in a diverse population living in San Diego, California: A g-computation application.

IntroductionGrowing evidence exists that greenspace exposure can reduce metabolic syndrome risk, a growing public health concern with well-documented inequities across population subgroups. We capitalize on the use of g-computation to simulate the influence of multiple possible interventions on residential greenspace on nine metabolic biomarkers and metabolic syndrome in adults (N = 555) from the 2014-2017 Community of Mine Study living in San Diego County, California.MethodsNormalized difference vegetation index (NDVI) exposure from 2017 was averaged across a 400-m buffer around the participants residential addresses. Participants fasting plasma glucose, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and triglyceride concentrations, systolic and diastolic blood pressure, hemoglobin A1c (%), waist circumference, and metabolic syndrome were assessed as outcomes of interest. Using parametric g-computation, we calculated risk differences for participants being exposed to each decile of the participant NDVI distribution compared to minimum NDVI. Differential health impacts from NDVI exposure by sex, ethnicity, income, and age were examined.ResultsWe found that a hypothetical increase in NDVI exposure led to a decrease in hemoglobin A1c (%), glucose, and high-density lipoprotein cholesterol concentrations, an increase in fasting total cholesterol, low-density lipoprotein cholesterol, and triglyceride concentrations, and minimal changes to systolic and diastolic blood pressure, waist circumference, and metabolic syndrome. The impact of NDVI changes was greater in women, Hispanic individuals, and those under 65 years old.ConclusionsG-computation helps to simulate the potential health benefits of differential NDVI exposure and identifies which subpopulations can benefit most from targeted interventions aimed at minimizing health disparities

The Interprofessional Management Of Geriatric Patients Undergoing Head And Neck Cancer Treatment In U.S. Nursing Homes

A dental approach toward interprofessional management, treatment planning and rehabilitation of diagnosed individuals undergoing cancer therapies is essential. Literature review focuses on presurgery oral hygiene instructions, pre-radiography/chemotherapy dental clearance, complications and management during radiotherapy/chemotherapy; post-radiotherapy/ chemotherapy oral healthcare; and adjunctive measures

The p38 MAPK pathway is essential for skeletogenesis and bone homeostasis in mice

Nearly every extracellular ligand that has been found to play a role in regulating bone biology acts, at least in part, through MAPK pathways. Nevertheless, much remains to be learned about the contribution of MAPKs to osteoblast biology in vivo. Here we report that the p38 MAPK pathway is required for normal skeletogenesis in mice, as mice with deletion of any of the MAPK pathway member–encoding genes MAPK kinase 3 (Mkk3), Mkk6, p38a, or p38b displayed profoundly reduced bone mass secondary to defective osteoblast differentiation. Among the MAPK kinase kinase (MAP3K) family, we identified TGF-β–activated kinase 1 (TAK1; also known as MAP3K7) as the critical activator upstream of p38 in osteoblasts. Osteoblast-specific deletion of Tak1 resulted in clavicular hypoplasia and delayed fontanelle fusion, a phenotype similar to the cleidocranial dysplasia observed in humans haploinsufficient for the transcription factor runt-related transcription factor 2 (Runx2). Mechanistic analysis revealed that the TAK1–MKK3/6–p38 MAPK axis phosphorylated Runx2, promoting its association with the coactivator CREB-binding protein (CBP), which was required to regulate osteoblast genetic programs. These findings reveal an in vivo function for p38β and establish that MAPK signaling is essential for bone formation in vivo. These results also suggest that selective p38β agonists may represent attractive therapeutic agents to prevent bone loss associated with osteoporosis and aging