Genetic analysis of TP53 in childhood myelodysplastic syndrome and juvenile myelomonocytic leukemia

信州大学博士（医学）・学位論文・平成23年3月31日授与（甲第886号)・齋藤章治ArticleLEUKEMIA RESEARCH. 35(12):1578-1584 (2011)journal articl

A comparison of dust concentration with high resolution analysis and visible strata in the Holocene and Last Glacial Maximum periods from the Dome Fuji ice core, East Antarctica

第3回極域科学シンポジウム　横断セッション「海・陸・氷床から探る後期新生代の南極寒冷圏環境変動」11月26日（月）、27日（火） ２階ラウン

Initial Results for Science Instruments Onboard EQUULEUS During the Cruising Phase Toward the Earth Moon Lagrange Point

EQUULEUS (EQUilibriUm Lunar-Earth point 6U Spacecraft) is a spacecraft to explore the cis-lunar region including the Earth-Moon Lagrange point L2 (EML2). The spacecraft is being jointly developed by JAXA, the University of Tokyo, and several other universities in Japan. After being launched into a lunar transfer orbit by NASA\u27s SLS (Space Launch System) Artemis-1 on November 16, 2022, the spacecraft successfully performed a first Delta-V and a trajectory correction maneuver. This enabled a precise lunar flyby and successful insertion into the orbit toward EML2. Although the size of EQUULEUS is only 6U CubeSat, the spacecraft carries three different science instruments. The spacecraft can effectively demonstrate science missions during and after the flight to EML2 by using these instruments; the plasmasphere observation around the Earth by PHOENIX, the space dust flux detection in the cis-lunar region by CLOTH, and the lunar impact flash (LIF) observation at the far side of the moon by DELPHINUS. All instruments have already completed its checkout. During the cruising phase, PHOENIX conducted Earth observations and successfully identified the Earth\u27s plasmashere. CLOTH has started regular standby operations. DELPHINUS obtained impressive images such as the far side of the Moon at lunar closest approach and long-period comet, Comet ZTF. This poster presents the details of these scientific missions and the initial checkout and observation results of the science instruments

Low toxicity of a conditioning with 8-Gy total body irradiation, fludarabine and cyclophosphamide as preparative regimen for allogeneic hematopoietic stem cell transplantation in pediatric hematological malignancies

The definitive version is available at www.blackwell-synergy.comWe here report the efficacy and toxicity of a conditioning regimen with fractionated 8-Gy TBI, fludarabine, and cyclophosphamide in allogeneic HSCT for pediatric hematological malignancies. Among 22 children who received related or unrelated HSCT, nine were transplanted with refractory disease and/or from HLA two or more loci-mismatched family donors. None of the patients developed graft failure. The Seattle grading system revealed that 18 patients had no RRT, and the remaining patients had grade I gastrointestinal toxicity alone. The estimated overall survival and leukemia-free survival at two yr were 57.1% and 48.0%, respectively, in 10 patients with acute lymphoblastic leukemia; 91.7% and 71.3%, respectively, in 12 patients with myeloid leukemia. The incidence of TRM was 4.8% at two yr. The rates of RRT above grade II and TRM in an 8-Gy TBI-containing regimen were significantly lower than the data of historical control patients who underwent 12-Gy TBI and cyclophosphamide with or without etoposide. The intermediate-dose TBI-based conditioning regimen may confer successful engraftment combined with minimized RRT, although its efficacy should be further evaluated.ArticlePEDIATRIC TRANSPLANTATION. 13(6):737-745 (2009)journal articl

Introduction of the ZmDof1 gene into rice enhances carbon and nitrogen assimilation under low-nitrogen conditions

The excessive application of nitrogen fertilizer to maximize crop yields causes negative environmental effects such as pollution and ecological imbalance. To overcome this problem, researchers have attempted to improve the nitrogen assimilation capacity of crops. Maize Dof1 (ZmDof1) is a plant-specific transcription factor shown to promote nitrogen assimilation in Arabidopsis thaliana (Arabidopsis) even under nitrogen-deficient conditions. The present study examines the effect of the introduction of the ZmDof1 gene on carbon and nitrogen assimilation in rice. ZmDof1 induced the expression of phosphoenolpyruvate carboxylase (PEPC) genes in transgenic rice plants and transactivated the PEPC promoters in protoplast transient assays, showing similar effects in rice as in Arabidopsis. Transgenic rice expressing ZmDof1 and grown in the presence of 360 μm (nitrogen-sufficient) or 90 μm (nitrogen-deficient) of nitrogen concentrations showed modulation of metabolite content and gene expression associated with the anaplerotic pathway for the TCA cycle, suggesting an increased carbon flow towards nitrogen assimilation. Furthermore, increases in carbon and nitrogen amounts per seedling were found in Dof1 rice grown under nitrogen-deficient conditions. Nitrogen deficiency also resulted in the predominant distribution of nitrogen to roots, accompanied by significant increases in root biomass and modification of the shoot-to-root ratio. Measurement of the CO2 gas exchange rate showed a significant increase in the net photosynthesis rate in Dof1 rice under nitrogen-deficient conditions. Taken these together, the present study displayed that ZmDof1 expression in rice could induce gene expressions such as PEPC genes, modulate carbon and nitrogen metabolites, increase nitrogen assimilation and enhance growth under low-nitrogen conditions

Characterization of the K2-19 Multiple-Transiting Planetary System via High-Dispersion Spectroscopy, AO Imaging, and Transit Timing Variations

K2-19 (EPIC201505350) is an interesting planetary system in which two transiting planets with radii ~ 7 $R_{Earth}$ (inner planet b) and ~ 4 $R_{Earth}$ (outer planet c) have orbits that are nearly in a 3:2 mean-motion resonance. Here, we present results of ground-based follow-up observations for the K2-19 planetary system. We have performed high-dispersion spectroscopy and high-contrast adaptive-optics imaging of the host star with the HDS and HiCIAO on the Subaru 8.2m telescope. We find that the host star is relatively old (>8 Gyr) late G-type star ($T_{eff}$ ~ 5350 K, $M_s$ ~ 0.9 $M_{Sun}$, and $R_{s}$ ~ 0.9 $R_{Sun}$). We do not find any contaminating faint objects near the host star which could be responsible for (or dilute) the transit signals. We have also conducted transit follow-up photometry for the inner planet with KeplerCam on the FLWO 1.2m telescope, TRAPPISTCAM on the TRAPPIST 0.6m telescope, and MuSCAT on the OAO 1.88m telescope. We confirm the presence of transit-timing variations, as previously reported by Armstrong and coworkers. We model the observed transit-timing variations of the inner planet using the synodic chopping formulae given by Deck & Agol (2015). We find two statistically indistinguishable solutions for which the period ratios ($P_{c}/P_{b}$) are located slightly above and below the exact 3:2 commensurability. Despite the degeneracy, we derive the orbital period of the inner planet $P_b$ ~ 7.921 days and the mass of the outer planet $M_c$ ~ 20 $M_{Earth}$. Additional transit photometry (especially for the outer planet) as well as precise radial-velocity measurements would be helpful to break the degeneracy and to determine the mass of the inner planet