Transcriptional co-activator protein p100 interacts with snRNP proteins and facilitates the assembly of the spliceosome

Transcription and pre-mRNA splicing are the key nuclear processes in eukaryotic gene expression, and identification of factors common to both processes has suggested that they are functionally coordinated. p100 protein has been shown to function as a transcriptional co-activator for several transcription factors. p100 consists of staphylococcal nuclease (SN)-like and Tudor-SN (TSN) domains of which the SN-like domains have been shown to function in transcription, but the function of TSN domain has remained elusive. Here we identified interaction between p100 and small nuclear ribonucleoproteins (snRNP) that function in pre-mRNA splicing. The TSN domain of p100 specifically interacts with components of the U5 snRNP, but also with the other spliceosomal snRNPs. In vitro splicing assays revealed that the purified p100, and specifically the TSN domain of p100, accelerates the kinetics of the spliceosome assembly, particularly the formation of complex A, and the transition from complex A to B. Consistently, the p100 protein, as well as the separated TSN domain, enhanced the kinetics of the first step of splicing in an in vitro splicing assay in dose-dependent manner. Thus our results suggest that p100 protein is a novel dual function regulator of gene expression that participates via distinct domains in both transcription and splicing

Flow State at Impeller Inlet: Optimization of Conical Frustum Section of Elbow Inlet Conduit in Large Low-Lift Pump Station

In large low-lift pump stations, the pump assembly comprises an inlet conduit, a pump, and an outlet conduit. A short conical frustum section that connects the elbow section with the impeller inlet directly affects the impeller inflow state, thereby influencing the overall performance. Therefore, investigating the conical frustum section contributes to studying the effect of inflow states on the performances of pump assemblies and similar pumping systems. To improve the pump assembly efficiency, three parameters of the conical frustum section, i.e., the contraction angle, height, and centerline inclination angle, are investigated and optimized via univariate and multivariate analyses. The flow field and external characteristics of the pump assembly are investigated via computational fluid dynamics simulation with a constant head. Furthermore, a comprehensive analysis and discussion of the performance improvement mechanisms are presented. The results indicate that the axial velocity distribution at the impeller inlet conforming to the cascade high-efficiency characteristics will achieve a better pump performance compared with a uniform distribution. The pump efficiency distribution can be predicted and visualized based on the cascade efficiency characteristics and the flow state at the impeller inlet using a machine learning method. In addition, the directions and distribution of the lateral and axial components of the inflow velocities have great impacts on the circulation distribution. A sensible circulation distribution at the guide vane outlet can suppress the entropy production and reduce hydraulic loss of the outlet conduit. In this case, a significant increase in the pump assembly efficiency is obtaine

Chinese Herbal Extracts Mitigate Ammonia Generation in the Cecum of Laying Hens: An In Vitro Study

The objectives of the study were to screen one or several Chinese herbal extracts with good ammonia emission reduction effects using an in vitro gas production study. The study consisted of a control (without Chinese herbal extract), and 11 experimental groups with added cinnamon extract (CE), Osmanthus extract (OE), tangerine peel extract (TPE), dandelion extract (DE), Coptis chinensis extract (CCE), honeysuckle extract (HE), Pulsatilla root extract (PRE), yucca extract (YE), licorice extract (LE), Ginkgo biloba extract (GBE), or astragalus extract (AE). The results showed that HE, PRE, YE, LE, GBE, and AE significantly reduced ammonia production (p ≤ 0.05). The most significant ammonia inhibition was achieved via AE, resulting in a 26.76% reduction. In all treatments, Chinese herbal extracts had no significant effect on pH, conductivity, or uric acid, urea, and nitrate-nitrogen concentrations (p > 0.05). However, AE significantly reduced urease activity and the relative activity of uricase (p ≤ 0.05). AE significantly increased the relative abundance of Bacteroides and decreased the relative abundance of Clostridium, Desulfovibrio, and Prevotell (p ≤ 0.05). Astragalus extract inhibited ammonia emission from laying hens by changing the gut microbial community structure, reducing the relative abundance of ammonia-producing bacteria, and reducing microorganisms’ uricase and urease activities

Contribution of Fertilizer, Density and Row Spacing Practices for Maize Yield and Efficiency Enhancement in Northeast China

The research aimed to assess the contribution of fertilizer, density, and row spacing in integrated cultivation measures and identify their regulation mechanism on canopy architecture and factors in biomass accumulation in spring maize. Zhengdan 958 was used as the experimental material, and the optimum mode (OM) was identified based on a preliminary experiment, including the optimal fertilizer management, suitable plant density and wide-narrow row spacing, and dramatic yield performance (11,445.16 kg ha−1 in 2017). Then, the effects of these practices on maize canopy structure performance were analyzed using the omission factors design experiment in optimum mode (OM). Treatments were set as follows: without fertilization (OM-F), without density (OM-D), and without wide-narrow plant spacing (OM-S). The results showed that the contribution of fertilization was maximum (23.85%), the second was intensive planting (16.05%), which promoted nitrogen accumulation and transport in leaves and stems via increased leaf area index and dry matter accumulation around the anthesis simultaneously, elevating the radiation utilization efficiency of the canopy and allowing a higher grain weight to be obtained. Wide-narrow row spacing yield contribution is minimum among the measures (8.649%), which could regulate the leaf and radiation transmittance in the middle and bottom layer of the canopy, while increasing the nitrogen accumulation of leaves and stalks in the silking stage, then significantly enhance the nitrogen transport and the matter accumulation of maize after anthesis. Our results showed that fertilizer management and density were the essential practices for integrated cultivation mode for northeast China. Moreover, wide-narrow row planting was advocated if permitted, which could elevate the utilization efficiency of radiation to 1%, and the yield of more than 11,000 kg ha−1 was obtained in Northeast China

New insights into the mechanism of ammonia toxicity: Focus on Cactus

The NF-κB signaling pathway is the most critical pathway in innate immunity. IκB (Cactus) is the primary cytoplasmic inhibitor of NF-κB (Dorsal). In this study, we found that ammonia exposure could significantly induce the expression of Cactus, in a dose-dependent manner in different tissues, with the highest expression in the gill of Corbicula fluminea. The expression pattern-related elements (Tube and Dorsal) in the NF-κB signaling pathway were also analyzed, showing significant up-regulation in 48 h. There was an inhibitory effect between up-regulated Cactus and Dorsal in 72 h, which may regulate Dorsal as a negative feedback pathway function to control the expression of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α). Besides, through molecular docking simulation, we found that the Cactus could be directly activated by NH3, complementing the regulatory mechanism of the Cactus. To further test our hypothesis, the levels of pro-inflammatory cytokines decreased after adding PDTC (the antioxidant of Cactus/IκB), suggesting that PDTC can prevent the degradation of Cactus, inhibit Dorsal translocating into the nucleus, and activate the pro-inflammatory cytokines. This revealed the inhibitory effect of Cactus on activating Dorsal/NF-κB factors in the NF-κB signaling pathway. Thus, we suggested that the Cactus is an essential regulator of ammonia-activated inflammation in C. fluminea, which was reported to be activated only by bacteria and immune stimulators. Our study provides a new perspective on the mechanism of ammonia toxicity in invertebrates