Experimental Study on Effects of Physical Exercise Function Training on Physical Health of Primary School Students

Recently, the physical health problems of children in China have attracted extensive attention. The education department has issued a notice on further strengthening the physical health management of primary and secondary schools. Many studies have shown that physical exercise function training has unique advantages in promoting the healthy development of students\u27 physique and is unaffected by venues or weather. This study aims to explore and verify the intervention effect of physical exercise function training on students’ physical health indicators, including physical shape, function, and quality. It also intends to practically explore the improvement of students\u27 physical health status and expand their physical education and health curriculum. Sixty-four elementary school students were selected to participate in the experiment: 32 were in the experimental group and 32 in the control group. The experiment was conducted three times a week for 10 weeks. The experimental group performed physical exercise function training teaching. The control group underwent routine PE teaching. Before and after the experiment, the compulsory indexes of Grade 4 in the national physical health standard for students (revised in 2014) were selected for the test, including height, weight, vital capacity, 50m running, sitting forward flexion, 1-minute rope skipping, and 1-minute sit-ups. Body shape, physical function, and physical quality of the two groups were measured and analyzed. Independent sample t-tests showed that there was no significant difference in body shape, physical function, and physical quality between the two groups before the experiment, and no statistically significant difference in body shape between the experimental and control groups after the experiment (p \u3e 0.05). The tests also showed that the physical function of the experimental group improved significantly compared to the control group after the experiment (t lung capacity = -2.346, p = 0.022). The experimental group improved significantly more than the control group in the 50m running, 1-minute rope skipping, and 1-minute sit-ups (t 50m running = -2.192, p = 0.032; t 1-minute rope skipping = 2.071, p = 0.043; t 1-minute sit ups = 2.035, p = 0.046). No statistically significant difference was found in sitting forward flexion between the experimental and control groups after the experiment (t sitting forward flexion = 1.555, p = 0.125). The exercise intervention has no effects on improving primary school students’ body shape. However, it has a significant effect on improving their physical function, and their vital capacity is significantly enhanced. The intervention effectively improved students’ 50m running, 1-minute rope skipping, and 1-minute sit-ups performance. In the control group, the physical health levels were slightly improved in routine physical education teaching. Therefore, designing a special physical exercise function training program is reasonable for improving the physical health of primary school students

Up-Regulation of Glioma-Associated Oncogene Homolog 1 Expression by Serum Starvation Promotes Cell Survival in ER-Positive Breast Cancer Cells

Background/Aims: Cancer cells are resistant to ischemia and starvation. Glioma-associated oncogene homolog 1 (Gli1) is a positive transcriptional activator of Hedgehog (Hh) pathway and plays an essential role in the development of cancers, including breast cancer. However, how Gli1 promotes cell survival remains elusive. The main purpose of this study is to investigate the pro-survival effect of Gli1 under serum starvation and its molecular mechanism in ER-positive breast cancer cells. Methods: Gene expression was determined by quantitative real-time PCR (QRT-PCR) and Western blot. The survival of Gli1 stably transfected ER-positive breast cancer cell lines (Gli1-MCF-7 and Gli1-T47D cells) and their untransfected control cells was estimated by WST-8 assay. Microarray analysis was performed to screen downstream Hh/Gli1 target genes in Gli1-overexpressed MCF-7 cells. Transcriptional activities of NF-kappaB were measured by luciferase assays. ChIP analysis was performed to explore whether cIAP2 was a direct target gene of Gli1. Results: Serum starvation significantly up-regulated the expression of Gli1 gene through activating PI3K/AKT pathway. Over-expression of Gli1 markedly promoted cell survival under serum starvation. Microarray analysis revealed that 338 genes were differentially expressed in Gli1-MCF-7 cells compared with those in the control cells. Among these genes, cellular inhibitor of apoptosis 2 (cIAP2), coding an anti-apoptosis and pro-survival protein, was significantly up-regulated not only by Hh/Gli1 pathway, but also by serum starvation. However, ChIP assay revealed no binding of Gli1 to cIAP2 promoter at the region of -1792 to -1568bp. Moreover, over-expression of Gli1 resulted in enhanced trans-activation of transcriptional factor NF-κB. Suppression of NF-κB signaling with NF-κB inhibitor Bay11-7082, significantly reduced the expression of cIAP2 and the cell survival under serum starvation. Conclusion: Serum starvation significantly up-regulated the expression of Gli1, which in turn increased its key target cIAP2 expression and enhanced NF-κB/cIAP2 pathway, resulting in promoting cell survival under serum starvation. These findings may provide new insights into the pro-survival mechanisms of Gli1 in breast cancer

A retrospective study on the analysis of influencing factors of neutropenia in endometrial cancer with adjuvant chemoradiotherapy

Abstract Objective This retrospective study aimed to investigate the factors influencing the occurrence of neutropenia in patients with endometrial cancer (EC) following adjuvant chemoradiotherapy (CRT). Methods Retrospective analysis of EC patients who underwent adjuvant CRT from January 2012 to June 2023 in the Department of Gynecology and Oncology of the First Affiliated Hospital of Shandong First Medical University. Neutropenia was defined as an Absolute Neutrophil Count (ANC) of peripheral blood neutrophils below 2 × 109/L. Factors affecting neutropenia in EC patients treated with CRT using Generalized Estimating Equation (GEE), and Logistic regression was used to further analyze the effect of adding radiotherapy to different chemotherapy cycles on neutropenia, so that patients receive optimal adjuvant CRT while the risk of neutropenia is appropriately controlled. Results A total of 144 patients met the inclusion criteria. They underwent 330 cycles of adjuvant chemotherapy, of whom 96 (66.7%) developed neutropenia, which occurred 140 times. The results of one-way GEE analysis showed that before CRT, White Blood Cell (WBC) (OR = 0.827; 95%CI, 0.701–0.976), ANC (OR = 0.749; 95%CI, 0.586–0.957), Absolute Monocyte Count (AMC) (OR = 0.047; 95%CI, 0.008–0.283), Blood Urea Nitrogen (BUN) (OR = 0.857; 95%CI, 0.741–0.991), platinum and docetaxel (platinum/docetaxel) dosing regimen (OR = 2.284; 95%CI, 1.130–4.618) were associated with neutropenia with adjuvant CRT for EC (p < 0.05), results of multifactorial GEE analysis showed that before adjuvant CRT ANC (OR = 0.552; 95%CI, 0.973–2.231), AMC (OR = 0.047; 95%CI, 0.004–0.052), platinum/docetaxel (OR = 2.437; 95%CI, 1.087–5.464) were an independent influence on neutropenia in adjuvant CRT for EC (p < 0.05). Multifactorial Logistic regression shows addition of radiotherapy to the first cycle of chemotherapy (OR = 4.413; 95%CI, 1.238–18.891) was an independent influence of neutropenia (p < 0.05). Conclusions Patients with low pre-CRT ANC and AMC, platinum/docetaxel dosing regimens need to be closely monitored during each cycle of CRT. Also, the concurrent addition of radiotherapy should be avoided during the first cycle of chemotherapy

A study on the characteristics of Songyang Duanwu tea, a classic traditional knowledge of Southwest Zhejiang, China

Duanwu Tea is a popular and ancient biodiversity-related traditional knowledge in Songyang, China. It has the remarkable function of dispelling dampness and relieving summer heat. However, systematic research on Duanwu Tea has been limited, and its biodiversity characteristics remain relatively unknown beyond the borders of Songyang. This study aims to investigate the bio-resources involved in making Duanwu Tea and its correlation with local geography and social conditions. To achieve this, semi-structured interviews were conducted and 23 formulations of Duanwu Tea were collected from the northern, central, and southern regions of Songyang. The results indicated that: (1) A total of 49 species of bio-resources belonging to 37 families and 46 genera were involved in the formulations. (2) There were no significant differences in the amounts of bio-resources in formulations between various regions. However, there were significant differences in the diversity indices of richness, Shannon-Weiner diversity, and Simpson with the diversity indices descending in the order of southern, central, and northern regions. (3) In terms of the processing of stems, leaves, flowers, and fruits as medicinal ingredients, there were significant differences between the regions. We concluded that the biodiversity characteristics of Duanwu Tea formula are related to the geographical environment, which provides a reference for further study and an opportunity for sustainable utilization. Through this study, we hope more people will pay attention to this valuable traditional knowledge and give it adequate protection and inheritance in the future