64 research outputs found

    Examining the assembly pathways and active microtubule mechanics underlying spindle self-organization

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    The bipolar organization of the microtubule-based mitotic spindle is essential for the faithful segregation of chromosomes in cell division. Despite our extensive knowledge of genes and proteins, the physical mechanism of how the ensemble of microtubules can assemble into a proper bipolar shape remains elusive. Here, we study the pathways of spindle self-organization using cell-free Xenopus egg extracts and computer-based automated shape analysis. Our microscopy assay allows us to simultaneously record the growth of hundreds of spindles in the bulk cytoplasm and systematically analyze the shape of each structure over the course of self-organization. We find that spindles that are maturing into a bipolar shape take a route that is distinct from those ending up with faulty structures, such as those of a tripolar shape. Moreover, matured structures are highly stable with little occasions of transformation between different shape phenotypes. Visualizing the movement of microtubules further reveals a fraction of microtubules that assemble between extra poles and push the poles apart, suggesting the presence of active extensile force that prevents pole coalescence. Together, we propose that a proper control over the magnitude and location of the extensile, pole-pushing force is crucial for establishing spindle bipolarity while preventing multipolarity.Comment: 22 pages, 5 + 2 figure

    X-Ray and Near-Infrared Observations of GX 339-4 in the Low/Hard State with Suzaku and IRSF

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    X-ray and near-infrared (JJ-HH-KsK_{\rm s}) observations of the Galactic black hole binary GX 339--4 in the low/hard state were performed with Suzaku and IRSF in 2009 March. The spectrum in the 0.5--300 keV band is dominated by thermal Comptonization of multicolor disk photons, with a small contribution from a direct disk component, indicating that the inner disk is almost fully covered by hot corona with an electron temperature of β‰ˆ\approx175 keV. The Comptonizing corona has at least two optical depths, Ο„β‰ˆ1,0.4\tau \approx 1,0.4. Analysis of the iron-K line profile yields an inner disk radius of (13.3βˆ’6.0+6.4)Rg(13.3^{+6.4}_{-6.0}) R_{\rm g} (RgR_{\rm g} represents the gravitational radius GM/c2GM/c^2), with the best-fit inclination angle of β‰ˆ50∘\approx50^\circ. This radius is consistent with that estimated from the continuum fit by assuming the conservation of photon numbers in Comptonization. Our results suggest that the standard disk of GX 339--4 is likely truncated before reaching the innermost stable circular orbit (for a non rotating black hole) in the low/hard state at ∼\sim1% of the Eddington luminosity. The one-day averaged near-infrared light curves are found to be correlated with hard X-ray flux with FKs∝FX0.45F_{\rm Ks} \propto F_{\rm X}^{0.45}. The flatter near infrared Ξ½FΞ½\nu F_{\nu} spectrum than the radio one suggests that the optically thin synchrotron radiation from the compact jets dominates the near-infrared flux. Based on a simple analysis, we estimate the magnetic field and size of the jet base to be 5Γ—1045\times10^4 G and 6Γ—1086\times 10^8 cm, respectively. The synchrotron self Compton component is estimated to be approximately 0.4% of the total X-ray flux.Comment: 17pages, 15 figures, accepted for publication in PASJ (Suzaku and MAXI Special Issue

    Outburst of LS V+44 17 Observed by MAXI and RXTE, and Discovery of a Dip Structure in the Pulse Profile

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    We report on the first observation of an X-ray outburst of a Be/X-ray binary pulsar LS V +44 17/RX J0440.9+4431, and the discovery of an absorption dip structure in the pulse profile. An outburst of this source was discovered by MAXI GSC in 2010 April. It was the first detection of the transient activity of LS V +44 17 since the source was identified as a Be/X-ray binary in 1997. From the data of the follow-up RXTE observation near the peak of the outburst, we found a narrow dip structure in its pulse profile which was clearer in the lower energy bands. The pulse-phase-averaged energy spectra in the 3βˆ’-100 keV band can be fitted with a continuum model containing a power-law function with an exponential cutoff and a blackbody component, which are modified at low energy by an absorption component. A weak iron KΞ±\alpha emission line is also detected in the spectra. From the pulse-phase-resolved spectroscopy we found that the absorption column density at the dip phase was much higher than those in the other phases. The dip was not seen in the subsequent RXTE observations at lower flux levels. These results suggest that the dip in the pulse profile originates from the eclipse of the radiation from the neutron star by the accretion column.Comment: 18 pages, 7 figures, accepted for publication in PAS

    Long-term Monitoring of the Black Hole Binary GX 339-4 in the High/Soft State during the 2010 Outburst with MAXI/GSC

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    We present the results of monitoring the Galactic black hole candidate GX 339-4 with the Monitor of All-sky X-ray Image (MAXI) / Gas Slit Camera (GSC) in the high/soft state during the outburst in 2010. All the spectra throughout the 8-month period are well reproduced with a model consisting of multi-color disk (MCD) emission and its Comptonization component, whose fraction is <= 25% in the total flux. In spite of the flux variability over a factor of 3, the innermost disk radius is constant at R_in = 61 +/- 2 km for the inclination angle of i = 46 deg and the distance of d=8 kpc. This R_in value is consistent with those of the past measurements with Tenma in the high/soft state. Assuming that the disk extends to the innermost stable circular orbit of a non-spinning black hole, we estimate the black hole mass to be M = 6.8 +/- 0.2 M_sun for i = 46 deg and d = 8 kpc, which is consistent with that estimated from the Suzaku observation of the previous low/hard state. Further combined with the mass function, we obtain the mass constraint of 4.3 M_sun < M < 13.3 M_sun for the allowed range of d = 6-15 kpc and i < 60 deg. We also discuss the spin parameter of the black hole in GX 339-4 by applying relativistic accretion disk models to the Swift/XRT data.Comment: 9 pages, 8 figures, accepted for publication in PASJ (Suzaku+MAXI special issue

    A Spectral Study of the Black Hole Candidate XTE J1752-223 in the High/Soft State with MAXI, Suzaku and Swift

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    We report on the X-ray spectral analysis of the black hole candidate XTE\ J1752--223 in the 2009--2010 outburst, utilizing data obtained with the MAXI/Gas Slit Camera (GSC), the Swift/XRT, and Suzaku, which work complementarily. As already reported by Nakahira et al. (2010) MAXI monitored the source continuously throughout the entire outburst for about eight months. All the MAXI/GSC energy spectra in the high/soft state lasting for 2 months are well represented by a multi-color disk plus power-law model. The innermost disk temperature changed from ∼\sim0.7 keV to ∼\sim0.4 keV and the disk flux decreased by an order of magnitude. Nevertheless, the innermost radius is constant at ∼\sim41 D3.5(cos⁑i)βˆ’1/2D_{3.5}(\cos{\it i})^{-1/2} km, where D3.5D_{3.5} is the source distance in units of 3.5 kpc and ii the inclination. The multi-color disk parameters obtained with the MAXI/GSC are consistent with those with the Swift/XRT and Suzaku. The Suzaku data also suggests a possibility that the disk emission is slightly Comptonized, which could account for broad iron-K features reported previously. Assuming that the obtained innermost radius represents the innermost stable circular orbit for a non-rotating black hole, we estimate the mass of the black hole to be 5.51Β±\pm0.28 MβŠ™M_{\odot} D3.5(cos⁑i)βˆ’1/2D_{3.5}(\cos{\it i})^{-1/2}, where the correction for the stress-free inner boundary condition and color hardening factor of 1.7 are taken into account. If the inclination is less than 49∘^{\circ} as suggested from the radio monitoring of transient jets and the soft-to-hard transition in 2010 April occurred at 1--4% of Eddignton luminosity, the fitting of the Suzaku spectra with a relativistic accretion-disk model derives constraints on the mass and the distance to be 3.1--55 MβŠ™M_{\odot} and 2.3--22 {\rm kpc}, respectively. This confirms that the compact object in XTE J1752--223 is a black hole.Comment: 12 pages including 7 figures and 4 tables, accepted for publication in PAS

    A Large X-ray Flare from a Single Weak-lined T Tauri Star TWA-7 Detected with MAXI GSC

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    We present a large X-ray flare from a nearby weak-lined T Tauri star TWA-7 detected with the Gas Slit Camera (GSC) on the Monitor of All-sky X-ray Image (MAXI). The GSC captured X-ray flaring from TWA-7 with a flux of 3Γ—10βˆ’93\times10^{-9} ergs cmβˆ’2^{-2} sβˆ’1^{-1} in 2--20 keV band during the scan transit starting at UT 2010-09-07 18:24:30.The estimated X-ray luminosity at the scan in the energy band is 3Γ—1032\times10^{32} ergs sβˆ’1^{-1},indicating that the event is among the largest X-ray flares fromT Tauri stars.Since MAXI GSC monitors a target only during a scan transit of about a minute per 92 min orbital cycle, the luminosity at the flare peak might have been higher than that detected. At the scan transit, we observed a high X-ray-to-bolometric luminosity ratio, log LX/LbolL_{\rm X}/L_{\rm bol} = βˆ’0.1βˆ’0.3+0.2-0.1^{+0.2}_{-0.3}; i.e., the X-ray luminosity is comparable to the bolometric luminosity. Since TWA-7 has neither an accreting disk nor a binary companion, the observed event implies that none of those are essential to generate such big flares in T Tauri stars.Comment: 4 pages, 2 figures, 1 table accepted for publication in PAS
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