20 research outputs found
A Measurement of the W Boson Mass
We report a measurement of the W boson mass based on an integrated luminosity
of 82 pb from \ppbar collisions at TeV recorded in
1994--1995 by the \Dzero detector at the Fermilab Tevatron. We identify W
bosons by their decays to and extract the mass by fitting the transverse
mass spectrum from 28,323 W boson candidates. A sample of 3,563 dielectron
events, mostly due to Z to ee decays, constrains models of W boson production
and the detector. We measure \mw=80.44\pm0.10(stat)\pm0.07(syst)~GeV. By
combining this measurement with our result from the 1992--1993 data set, we
obtain \mw=80.43\pm0.11 GeV.Comment: 11 pages, 5 figure
Influence of Dll4 via HIF-1α-VEGF Signaling on the Angiogenesis of Choroidal Neovascularization under Hypoxic Conditions
Choroidal neovascularization (CNV) is the common pathological basis of
irreversible visual impairment encountered in a variety of chorioretinal
diseases; the pathogenesis of its development is complicated and still
imperfectly understood. Recent studies indicated that delta-like ligand 4
(Dll4), one of the Notch family ligands might participate in the HIF-1α-VEGF
pathway to regulate CNV angiogenesis. But little is known about the influence
and potential mechanism of Dll4/Notch signals on CNV angiogenesis. Real-time
RT-PCR, Western blotting were used to analyze the expression alteration of Dll4,
VEGF and HIF-1α in hypoxic RF/6A cells. Immunofluorescence staining, a
laser-induced rat CNV model and intravitreal injection techniques were used to
confirm the relationships among these molecules in vitro and
in vivo. RPE-RF/6A cell co-culture systems were used to
investigate the effects of Dll4/Notch signals on CNV angiogenesis. We found that
the Dll4 was involved in hypoxia signaling in CNV angiogenesis. Results from the
co-culture system showed that the enhancement of Dll4 expression in RF/6A cells
led to the significantly faster proliferation and stronger tube forming ability,
but inhibited cells migration and invasion across a monolayer of RPE cells in
hypoxic environment, while siRNA-mediated Dll4 silencing caused the opposite
effects. Pharmacological disruption of Notch signaling using gamma-secretase
inhibitor (GSI) produced similar, but not identical effects, to that caused by
the Dll4 siRNA. In addition, the expression of several key molecules involved in
the angiogenesis of CNV was altered in RF/6A cells showing constitutively active
Dll4 expression. These results suggest that Dll4 play an important role in CNV
angiogenesis, which appears to be regulated by HIF-1α and VEGF during the
progression of CNV under hypoxic conditions. Targeting Dll4/Notch signaling may
facilitate further understanding of the mechanisms that underlie CNV
angiogenesis
Epigenetics of osteoarthritis: is DNA Dmethylation involved in the pathogenesis of the disease?
Efficacy of expired foot-and-mouth disease O type vaccines in cattle and buffalo in Lao People's Democratic Republic
Lao People's Democratic Republic (Lao PDR) submitted a request to Japan for 200,000 doses of expired foot-and-mouth disease (FMD) O type vaccines that were in storage for emergency use. Approximately 100,000 animals, consisting of both cattle and Asian water buffalo (Bubalus bubalus bubalis), received the same vaccine twice within one month in Xieng Khouang province in the northeast area of Lao PDR. Concurrently, the efficacy of three-month expired FMD O type vaccine (6PD50 O Manisa) was assessed in serum samples of 90 cattle and 31 buffalo from the field using a Liquid Phase Blocking-ELISA (LPBE) assay. Of these samples, 75 cattle (83.3%) and 24 buffalo (77.4%) were seropositive against the FMD virus (FMDV) O type before vaccination. Testing for non-structural protein (NSP) using the PrioCHECK FMD NS kit showed that many of the animals with high titers in the screening test before vaccination were FMDV-infected animals. Fifteen cattle and seven buffalo with titers 1:32 or under before vaccination exhibited high titers of antibody (1:45-1:1448) one month after the first vaccination and further increased titers (1:362-1:5792) one month after the second vaccination. Nearly all of the cattle (97.6%) had high titers to control FMD 14 months after the second vaccination. To date, no outbreak of FMD has been reported at the study site. Three-month expired FMD O type vaccines induced appropriate immune responses against FMD in both cattle and buffalo