LC-ESI-HRMS-Based Chemical Characterization of Lupinus bogotensis Roots

Plants of the genus Lupinus (Fabaceae) have been studied due to the occurrence of different compounds, especially those possessing quinolizidine and isoflavone-like structures. These kinds of compounds are characterized by both medical and industrial applications, providing various benefits to human being. However, organs such as roots have not been equally studied and there is a lack of such records for native species. Therefore, in the present study, the chemical composition of nodulated roots from greenhouse-established L. bogotensis plants was determined. The resulting crude ethanolic extract was then analyzed by LC/HRMS and chemical nature of most compounds was determined by analyzing the high resolution mass spectra. Recorded profile showed adequate separation allowing tentative identification of detected compounds. 47 secondary metabolites (mainly isoflavones and quinolizidine-type compounds) were thus identified. Most phenolic compounds were found to be conjugated flavonoids (e.g., genistin and genistein malonylglucoside) and lupanine, sparteine and hydroxylupanine were noticed as the main alkaloids. Among alkaloid-like compounds, dehydromitomycin C, a compound produced by Streptomyces caespitosus was identified. Lupadienediol (a lupane-type triterpene recognized for being involved in rhizobacteria:legumes symbiosis) was the only terpene-related component identified in the extract. The present work corresponds to the first report on the chemical composition of L. bogotensis root and constitutes an adequate basis for phytoconstituents finding from nature to support the use of native species