Cepstral analysis based on the Glimpse proportion measure for improving the intelligibility of HMM-based synthetic speech in noise

In this paper we introduce a new cepstral coefficient extraction method based on an intelligibility measure for speech in noise, the Glimpse Proportion measure. This new method aims to increase the intelligibility of speech in noise by modifying the clean speech, and has applications in scenarios such as public announcement and car navigation systems. We first explain how the Glimpse Proportion measure operates and further show how we approximated it to integrate it into an existing spectral envelope parameter extraction method commonly used in the HMM-based speech synthesis framework. We then demonstrate how this new method changes the modelled spectrum according to the characteristics of the noise and show results for a listening test with vocoded and HMM-based synthetic speech. The test indicates that the proposed method can significantly improve intelligibility of synthetic speech in speech shaped noise. Index Terms — cepstral coefficient extraction, objective measure for speech intelligibility, Lombard speech, HMM-based speech synthesis 1

Uma ferramenta WEB para identificação e visualização de sequências repetitivas em lócus gênico de animais e plantas.

Sequências repetitivas (SR) em lócus gênico de animais e plantas podem estar envolvidas em diversos fenômenos biológicos, como interferência por RNAi (Fire et al., 1998) e trans-splicing (Di Segni et al., 2008). A maioria das SR localiza-se em regiões de introns e, usualmente, seus estudos envolvem apenas sequências de mRNA, sendo necessário a estrutura intron-exon dos genes. Desta forma, é preciso realizar um mapeamento dos genes em seu respectivo genoma de referência para identificação de quatro tipos de SR: repetição reversa e complementar, repetição direta e complementar, repetição reversa e repetição direta. Este trabalho apresenta uma ferramenta WEB, chamada RepGraph, a qual integra algoritmos e ferramentas de bioinformática para identificar os pares que formam cada tipo de SR e uma representação gráfica para ilustrar sua relação em dois lócus gênicos.Trabalho apresentado na V Mostra de Trabalhos de Estagiários e Bolsistas, Campinas, out. 2009

Detection of human interchromosomal trans-splicing in sequence databanks.

Trans-splicing is a common phenomenon in nematodes and kinetoplastids, and it has also been reported in other organisms, including humans. Up to now, all in silico strategies to find evidence of trans-splicing in humans have required that the candidate sequences follow the consensus splicing site rules (spliceosome-mediated mechanism). However, this criterion is not supported by the best human experimental evidence, which, except in a single case, do not follow canonical splicing sites. Moreover, recent findings describe a novel alternative tRNA mediated trans-splicing mechanism, which prescinds the spliceosome machinery. In order to answer the question, ?Are there hybrid mRNAs in sequence databanks, whose characteristics resemble those of the best human experimental evidence??, we have developed a methodology that successfully identified 16 hybrid mRNAs which might be instances of interchromosomal trans-splicing. Each hybrid mRNA is formed by a trans-spliced region (TSR), which was successfully mapped either onto known genes or onto a human endogenous retrovirus (HERV-K) transcript which supports their transcription. The existence of these hybrid mRNAs indicates that trans-splicing may be more widespread than believed. Furthermore, non-canonical splice site patterns suggest that infrequent splicing sites may occur under special conditions, or that an alternative trans-splicing mechanism is involved. Finally, our candidates are supposedly from normal tissue, and a recent study has reported that trans-splicing may occur not only in malignant tissues, but in normal tissues as well. Our methodology can be applied to 5'-UTR, coding sequences and 3'-UTR in order to find new candidates for a posteriori experimental confirmation

Dilepton and Photon Emission Rates from a Hadronic Gas III

We extend our early analyses of the dilepton and photon emission rates from a hadronic gas to account for strange mesons using a density expansion. The emission rates are reduced to vacuum correlation functions using three-flavor chiral reduction formulas, and the latters are assessed in terms of empirical data. Using a fire-ball, we compare our results to the low and intermediate mass dilepton data available from CERN. Our results suggest that a baryon free hadronic gas does not account for the excess of low mass dielectrons observed at CERES but do well in accounting for the intermediate dimuons at HELIOS. The same observations apply to the recent low and high $p_t$ dielectron rates from CERES.Comment: 12 pages LaTeX, 11 eps figure

Um modelo orientado a objetos para o cálculo da estimativa da área acessível a solvente.

Estimativa da ASA. Descrição do método utilizado. Modelo orientado a objetos proposto.bitstream/CNPTIA/10655/1/doc46.pdfAcesso em: 29 maio 2008

Revisiting the security of speaker verification systems against imposture using synthetic speech

In this paper, we investigate imposture using synthetic speech. Although this problem was first examined over a decade ago, dramatic improvements in both speaker verification (SV) and speech synthesis have renewed interest in this problem. We use a HMM-based speech synthesizer which creates synthetic speech for a targeted speaker through adaptation of a background model. We use two SV systems: standard GMMUBM- based and a newer SVM-based. Our results show when the systems are tested with human speech, there are zero false acceptances and zero false rejections. However, when the systems are tested with synthesized speech, all claims for the targeted speaker are accepted while all other claims are rejected. We propose a two-step process for detection of synthesized speech in order to prevent this imposture. Overall, while SV systems have impressive accuracy, even with the proposed detector, high-quality synthetic speech will lead to an unacceptably high false acceptance rate

Análise preliminar de um processo para identificação e alinhamento de seqüências homólogas para proteínas com estrutura resolvida.

O objetivo deste trabalho é apresentar e fazer uma avaliação preliminar de um processo alternativo, denominado Sequences Homologue to the Query (Structure-having) Sequence-SH2Q, para elaboração de alinhamentos múltiplos semelhantes à aqueles relatados no HSSP. O processo aqui apresentado baseia-se em programas de domínio público para busca em bases de dados de sequências -Blast (Altschul et al., 1990, 1997) e para alinhamento múltiplo de sequências -ClustalW (Thompson et al., 1994) O critério para avaliação do mesmo é o grau de similaridade entre as medidas de Entropia Relativa, quando comparadas com os mesmos valores relatados pelo HSSP.bitstream/CNPTIA/10041/1/comtec48.pdfAcesso em: 30 maio 2008