トリフェニルアミン系有機エレクトロルミネッセンス材料の分子構造・電子状態と電荷輸送特性

京都大学0048新制・課程博士博士(工学)甲第16320号工博第3448号新制||工||1521(附属図書館)28937京都大学大学院工学研究科分子工学専攻(主査)教授 梶 弘典, 教授 田中 庸裕, 教授 佐藤 啓文学位規則第4条第1項該当Doctor of Philosophy (Engineering)Kyoto UniversityDFA

エンドウ原形質膜におけるATPアーゼとホスファチジルイノシトールリン資質リン酸化酵素の共精製

The plasma membrance ATPase was partially purified by a linear glycerol density gradient centrifugation of the detergent-solubilized plasma membrance poteins and subsequent separation by a size-exclusion column chromatogrphy. A purified ATPase preparation is shown to contain a 97.6kDa protein that was cross-reacted with an antibody raised against mung bean H+-ATPase. The preparation also exhibited the phosphorylation of exogenous phosphatidylionsitol(PI) when supplized with [γ-32P]ATP. These results indicate that one form plasma membrance ATPase is co-purified with PI kinase.エンドウの上胚軸組織から分離した原形質膜画分におけるATPアーゼとホスファチジルイノシトールリン脂質リン酸化酵素との相互作用を解析する目的で、双方の原形質膜画分からの可溶化とそれらの部分精製を試みた。原形質膜のTritonX－100可溶化画分をグリセロール連続密度勾配遠心分画に供し、得られた活性画分をさらに分子ふるいカラムクロマトグラフィーによって分離した。この結果、ＡＴＰアーゼとホスファチジルイイノシトールリン脂質リン酸化酵素は共精製され、非変性条件下では双方の活性を分けることができなかった

共生真菌アクレモニウムエンドファイトの形質転換

Conditions have been developed for transforming protoplasts of the Acremonium endophyte by PEG 4000 and electroporation. Transformation by PEG exhibited a higher number of transformants than by electroporation. lntegration of iaaM gene into the genome was examined by PCR and Southern blot hybridization analysis. PCR product showed that transformants banded at around 1.7 kb corresponding to the size of iaaM gene. Hybridization of the digests of genomic DNA with iaaM gene as DNA probe showed that the number of hybridized band signals was different between transformant and non-transformant. These results might indicate that PEG is an effective method for the transformation of Acremonium endophyte and that there are repeated copies of the iaaM homologous sequences in the genome of Acremonium.アクレモニウムエンドファイトの形質転換の条件を検討した。アクレモニウムエンドファイトからプロトプラストを調製し、PEG4000とエレクトロポレーションを用いて形質転換を試みた。その結果、PEGで形質転換すると、エレクトロポレーションによる場合よりも多くの形質転換体が得られた。PCR解析によってiaaM遺伝子のゲノムへの導入を確認したところ、形質転換体のPCR産物はiaaM遺伝子のサイズに相当する約1.7kbのバンドを持っていた。一方、iaaM遺伝子をプローブとしたサザンブロット解析においては、形質転換体と非形質転換体の間にはハイブリダイズした断片数に違いがあることが明らかとなった。以上の結果は、アクレモニウムエンドファイトの形質転換にはPEG法が有効であること、アクレモニウムエンドファイトのゲノム中にはiaaM遺伝子様配列の反復コピーが存在することを示唆している

Effect of increasing salinity on biogas production in waste landfills with leachate recirculation: A lab-scale model study

AbstractThe effects of salinity on anaerobic waste degradation and microbial communities were investigated, in order to propose an appropriate leachate recirculation process in a waste landfill in a tropical region. A salt concentration of 21mScm−1 of electrical conductivity (EC) did not affect waste degradation, but a salt concentration of 35mScm−1 of EC inhibited CH4 generation. A higher salt concentration of 80mScm−1 of EC inhibited not only CH4 and CO2 generation, but also degradation of organic compounds. The bacterial and archaeal community compositions were affected by high salinity. High salinity can exert selective pressure on bacterial communities, resulting in a change in bacterial community structure. Ammonium caused strong, dominant inhibition of biogas production in the salt concentration range of this study. Quality control, especially of ammonium levels, will be essential for the promotion of waste biodegradation in landfills with leachate recirculation

Association of progressive intracranial necrotic lesion with acute myelogenous leukemia: A case report

For patients with acute myelogenous leukemia (AML) and invasive central nervous system lesions, there are many possible differential diagnoses, including collagen diseases, infectious diseases, drug reactions or side effects, and vascular diseases. Herein, we describe the clinical course, diagnostic examinations, and treatment outcomes for a patient with AML complicated by a progressively enlarging intracranial necrotic lesion. As no tumor cells were seen on microscopic examination, and since the clinical symptoms improved after leukemia therapy, myeloid sarcoma was highly suspected. Taken together, this case suggests that a combination of the clinical course, radiological findings, and treatment history should be considered to eliminate other possible diagnoses