Ability Discovery and Weak Centralized Based Crowdsourcing Service Release System in Social Network

Crowdsourcing developed rapidly for its inspiring public abilities. But how to effectively find qualified participants and how to find and prevent malicious workers may be the main difficulties to ensure the crowdsourcing quality. In this paper, the related theories of social network were used in crowdsourcing services, the task publisher (Seeker) was regarded as the network center, his Abilities Set (AS) would be quantified and his Friends Abilities Matrix (FAM) would be generated according to the communication between them, thus his social network was re-constructed. Subsequently, some friends that conformed to the ability requirements of the task would be chosen to be the task receivers (Solvers). The natural trust relationship in the social network was fully used to build a crowdsourcing service release system on weak centralization. By using the social network, even the privacy information needn’t to be shared with others, the system could help the seeker find solvers accurately in the seeker’s own social network according to task demands, and then help to reduce fraud and invalid data. The simulation experiments showed that the release system could help the seeker discover his own abilities, construct the FAM, and select the appropriate solvers precisely and automatically

PAR-1 Kinase Phosphorylates Dlg and Regulates Its Postsynaptic Targeting at the Drosophila Neuromuscular Junction

SummaryTargeting of synaptic molecules to their proper location is essential for synaptic differentiation and plasticity. PSD-95/Dlg proteins have been established as key components of the postsynapse. However, the molecular mechanisms regulating the synaptic targeting, assembly, and disassembly of PSD-95/Dlg are not well understood. Here we show that PAR-1 kinase, a conserved cell polarity regulator, is critically involved in controlling the postsynaptic localization of Dlg. PAR-1 is prominently localized at the Drosophila neuromuscular junction (NMJ). Loss of PAR-1 function leads to increased synapse formation and synaptic transmission, whereas overexpression of PAR-1 has the opposite effects. PAR-1 directly phosphorylates Dlg at a conserved site and negatively regulates its mobility and targeting to the postsynapse. The ability of a nonphosphorylatable Dlg to largely rescue PAR-1-induced synaptic defects supports the idea that Dlg is a major synaptic substrate of PAR-1. Control of Dlg synaptic targeting by PAR-1-mediated phosphorylation thus constitutes a critical event in synaptogenesis

Attribution analysis of multi-temporal scale changes of streamflow in the source area of Lancang River with seasonal scale Budyko model

Under the influence of climate change and human activities, the intra-annual distribution characteristics of streamflow have changed, directly affecting the exploitation of water resources and the health of ecosystems. The trend-free pre-whitening Mann-Kendall (TFPW-MK) test method, concentration degree and concentration period, and Bernaola-Galvan (BG) segmentation algorithm were applied to analyze variation trend, intra-annual distribution characteristics, and abrupt year of streamflow. Then, the monthly water storage and monthly actual evaporation of the source area of the Lancang River (SALR) were calculated by the monthly ABCD model. Finally, the contributions of different factors to runoff variability at multiple time scales were quantified using the seasonal-scale Budyko hypothesis approach. The results showed that: (1) The runoff revealed a significant upward trend on the annual scale. Runoff exhibited a significant upward trend in January, October and November, and runoff in other months and seasons exhibited an insignificant upward trend. (2) The intra-annual distribution characteristics of runoff in the SALR showed an obvious “Single-peak type“ distribution, reaching a maximum in July and August. (3) The year of sudden change in streamflow was 2008. (4) The contribution of climate change and human activities to the annual runoff change was 83.3% and 16.7%, respectively. The degree of influence of climate change on runoff change was ranked as spring (96.8%), autumn (85.3%), winter (82.2%) and summer (58.2%). The order of impact of human activity on runoff change was summer (41.8%), winter (17.8%), autumn (14.7%), spring (3.2%)

Effects of Deep Tillage and Straw Returning on Soil Microorganism and Enzyme Activities

Two field experiments were conducted for two years with the aim of studying the effects of deep tillage and straw returning on soil microorganism and enzyme activity in clay and loam soil. Three treatments, (1) conventional tillage (CT), shallow tillage and straw returning; (2) deep tillage (DT), deep tillage and straw returning; and (3) deep tillage with no straw returning (DNT), were carried out in clay and loam soil. The results showed that deep tillage and straw returning increased the abundance of soil microorganism and most enzyme activities. Deep tillage was more effective for increasing enzyme activities in clay, while straw returning was more effective in loam. Soil microorganism abundance and most enzyme activities decreased with the increase of soil depth. Deep tillage mainly affected soil enzyme activities in loam at the soil depth of 20–30 cm and in clay at the depth of 0–40 cm. Straw returning mainly affected soil microorganism and enzyme activities at the depths of 0–30 cm and 0–40 cm, respectively

Prognostic and therapeutic significance of microbial cell-free DNA in plasma of people with acutely decompensated cirrhosis

BACKGROUND AND AIMS: Although the effect of bacterial infection on cirrhosis has been well-described, the effect of non-hepatotropic virus (NHV) infection is unknown. This study evaluated the genome fragments of circulating microorganisms using metagenomic next-generation sequencing (mNGS) in cirrhosis patients with acute decompensation (AD), focusing on NHVs and related the findings to clinical outcomes. METHODS: Plasma mNGS was performed in 129 cirrhosis patients with AD in study cohort. Ten healthy volunteers and 20, 39, and 81 patients with stable cirrhosis, severe sepsis and hematological malignancies, respectively, were enrolled as controls. Validation assays for human cytomegalovirus (CMV) reactivation in a validation cohort (n = 58) were performed and exploratory treatment instituted. RESULTS: In study cohort, 188 microorganisms were detected in 74.4% (96/129) patients, including viruses (58.0%), bacteria (34.1%), fungi (7.4%) and chlamydia (0.5%). Patients with AD had an NHV signature, and CMV was the most frequent NHV, which correlated with the clinical effect of empirical antibiotic treatment, progression to acute-on-chronic liver failure (ACLF), and 90-day mortality. The NHV signature in ACLF patients was similar to patients with sepsis and hematological malignancies. The treatable NHV, CMV was detected in 24.1% (14/58) patients in the validation cohort. Of the 14 cases with detectable CMV by mNGS, 9 were further validated by DNA RT-PCR or pp65 antigenemia testing. Three patients with CMV reactivation received ganciclovir therapy in exploratory manner with clinical resolutions. CONCLUSIONS: The results of this study suggests that NHVs may have a pathogenic role in complicating the course of AD. Further validation is needed to define whether this should be incorporated in the routine management of AD patients. IMPACT AND IMPLICATIONS: ●Cirrhosis patients with acute decompensation have a non-hepatotropic virus (NHV) signature, which is similar to that in sepsis and hematological malignancies patients. ●The detected viral signature had clinical correlates, including clinical efficacy of empirical antibiotic treatment, progression to acute-on-chronic liver failure and short-term mortality. ●The treatable NHV, CMV reactivation may be involved in the clinical outcomes of decompensated cirrhosis. ●Routine screening for NHVs, especially CMV, may be useful for the management of patients with acutely decompensated cirrhosis

The burden of heatwave-related preterm births and associated human capital losses in China

Frequent heatwaves under global warming can increase the risk of preterm birth (PTB), which in turn will affect physical health and human potential over the life course. However, what remains unknown is the extent to which anthropogenic climate change has contributed to such burdens. We combine health impact and economic assessment methods to comprehensively evaluate the entire heatwave-related PTB burden in dimensions of health, human capital and economic costs. Here, we show that during 2010-2020, an average of 13,262 (95%CI 6,962-18,802) PTBs occurred annually due to heatwave exposure in China. In simulated scenarios, 25.8% (95%CI 17.1%-34.5%) of heatwave-related PTBs per year on average can be attributed to anthropogenic climate change, which further result in substantial human capital losses, estimated at over $1 billion costs. Our findings will provide additional impetus for introducing more stringent climate mitigation policies and also call for more sufficient adaptations to reduce heatwave detriments to newborn

Material Removal Mechanism and Force Model of Nanofluid Minimum Quantity Lubrication Grinding

Numerous researchers have developed theoretical and experimental approaches to force prediction in surface grinding under dry conditions. Nevertheless, the combined effect of material removal and plastic stacking on grinding force model has not been investigated. In addition, predominant lubricating conditions, such as flood, minimum quantity lubrication (MQL), and nanofluid minimum quantity lubrication (NMQL), have not been considered in existing force models. In this study, material removal mechanism under different lubricating conditions was researched. An improved theoretical force model that considers material removal and plastic stacking mechanisms was presented. Grain states, including cutting and ploughing, are determined by cutting efficiency (β). The influence of lubricating conditions was also considered in the proposed force model. Simulation was performed to obtain the cutting depth (a g) of each “dynamic active grain.” Parameter β was introduced to represent the plastic stacking rate and determine the force algorithms of each grain. The aggregate force was derived through the synthesis of each single-grain force. Finally, pilot experiments were conducted to test the theoretical model. Findings show that the model’s predictions were consistent with the experimental results, with average errors of 4.19% and 4.31% for the normal and tangential force components, respectively

Transplacentally Acquired Maternal Antibody against Hepatitis B Surface Antigen in Infants and its Influence on the Response to Hepatitis B Vaccine

BACKGROUND: Passively acquired maternal antibodies in infants may inhibit active immune responses to vaccines. Whether maternal antibody against hepatitis B surface antigen (anti-HBs) in infants may influence the long-term immunogenicity of hepatitis B vaccine remains unknown. METHODOLOGY/PRINCIPAL FINDINGS: Totally 338 pairs of mothers and children were enrolled. All infants were routinely vaccinated against hepatitis B based on 0-, 1- and 6-month schedule. We characterized the transplacental transfer of maternal anti-HBs, and compared anti-HBs response in children of mothers with or without anti-HBs. In a prospective observation, all 63 anti-HBs positive mothers transferred anti-HBs to their infants; 84.1% of the infants had higher anti-HBs concentrations than their mothers. One and half years after vaccination with three doses of hepatitis B vaccine, the positive rate and geometric mean concentration (GMC) of anti-HBs in 32 infants with maternal anti-HBs were comparable with those in 32 infants without maternal antibody (90.6% vs 87.5%, P = 0.688, and 74.5 vs 73.5 mIU/ml, P = 0.742, respectively). In a retrospective analysis, five and half years after vaccination with three doses vaccine, the positive rates of anti-HBs in 88 children of mothers with anti-HBs ≥1000 mIU/ml, 94 children of mothers with anti-HBs 10-999 mIU/ml, and 61 children of mothers with anti-HBs <10 mIU/ml were 72.7%, 69.2%, and 63.9% (P = 0.521), respectively; anti-HBs GMC in these three groups were 38.9, 43.9, and 31.7 mIU/ml (P = 0.726), respectively. CONCLUSIONS/SIGNIFICANCE: The data demonstrate that maternal anti-HBs in infants, even at high concentrations, does not inhibit the long-term immunogenicity of hepatitis B vaccine. Thus, current hepatitis B vaccination schedule for infants will be still effective in the future when most infants are positive for maternal anti-HBs due to the massive vaccination against hepatitis B

Palmitoylation and membrane cholesterol stabilize μ-opioid receptor homodimerization and G protein coupling

<p>Abstract</p> <p>Background</p> <p>A cholesterol-palmitoyl interaction has been reported to occur in the dimeric interface of the β₂-adrenergic receptor crystal structure. We sought to investigate whether a similar phenomenon could be observed with μ-opioid receptor (OPRM1), and if so, to assess the role of cholesterol in this class of G protein-coupled receptor (GPCR) signaling.</p> <p>Results</p> <p>C3.55(170) was determined to be the palmitoylation site of OPRM1. Mutation of this Cys to Ala did not affect the binding of agonists, but attenuated receptor signaling and decreased cholesterol associated with the receptor signaling complex. In addition, both attenuation of receptor palmitoylation (by mutation of C3.55[170] to Ala) and inhibition of cholesterol synthesis (by treating the cells with simvastatin, a HMG-CoA reductase inhibitor) impaired receptor signaling, possibly by decreasing receptor homodimerization and Gαi2 coupling; this was demonstrated by co-immunoprecipitation, immunofluorescence colocalization and fluorescence resonance energy transfer (FRET) analyses. A computational model of the OPRM1 homodimer structure indicated that a specific cholesterol-palmitoyl interaction can facilitate OPRM1 homodimerization at the TMH4-TMH4 interface.</p> <p>Conclusions</p> <p>We demonstrate that C3.55(170) is the palmitoylation site of OPRM1 and identify a cholesterol-palmitoyl interaction in the OPRM1 complex. Our findings suggest that this interaction contributes to OPRM1 signaling by facilitating receptor homodimerization and G protein coupling. This conclusion is supported by computational modeling of the OPRM1 homodimer.</p