9 research outputs found
Applying Software Model Checking Techniques For Behavioral UML Models
Abstract. This work presents a novel approach for the verification of Behavioral UML models, by means of software model checking. We propose adopting software model checking techniques for verification of UML models. We translate UML to verifiable C code which preserves the high level structure of the models, and abstracts details that are not needed for verification. We combine of static analysis and bounded model checking for verifying LTL safety properties and absence of livelocks. We implemented our approach on top of the bounded software model checker CBMC. We compared it to an IBM research tool that verifies UML models via a translation to IBM's hardware model checker RuleBasePE. Our experiments show that our approach is more scalable and more robust for finding long counterexamples. We also demonstrate the usefulness of several optimizations that we introduced into our tool
The biochar effect: plant resistance to biotic stresses
Biochar (charcoal) is the solid co-product of pyrolysis, the thermal degradation of biomass in the absence of oxygen. Pyrolysis also yields gaseous and liquid biofuel products. There is a growing interest worldwide in the pyrolysis platform, for at least four reasons: (i) pyrolysis can be a source of renewable biofuels; (ii) many biomass waste materials can be treated by pyrolysis and thus converted into a fuel resource; (iii) long-term sequestration of carbon dioxide which originated in the atmosphere may result from adding biochar to soil; and (iv) biochar soil amendment contributes to improved soil fertility and crop productivity. Currently, however, very little biochar is utilized in agriculture, in part because its agronomic value in terms of crop response and soil health benefits have yet to be quantified, and because the mechanisms by which it improves soil fertility are poorly understood. The positive effects of biochar on crop productivity under conditions of extensive agriculture are frequently attributed to direct effects of biochar-supplied nutrients and to several other indirect effects, including increased water and nutrient retention, improvements in soil pH, increased soil cation exchange capacity, effects on P and S transformations and turnover, neutralization of phytotoxic compounds in the soil, improved soil physical properties, promotion of mycorrhizal fungi, and alteration of soil microbial populations and functions. Yet, the biochar effect is also evident under conditions of intensive production where many of these parameters are not limited. Biochar addition to soil alters microbial populations in the rhizosphere, albeit via mechanisms not yet understood, and may cause a shift towards beneficial microorganism populations that promote plant growth and resistance to biotic stresses. In addition to some scant evidence for biochar-induced plant protection against soilborne diseases, the induction of systemic resistance towards several foliar pathogens in three crop systems has been demonstrated. There are indications that biochar induces responses along both systemic acquired resistance (SAR) and induced systemic resistance (ISR) pathways, resulting in a broad spectrum controlling capacity in the canopy. This review examines the effects of biochar soil amendment on the different soil-plant-microbe interactions that may have a role in plant health. Improvement of plant responses to disease can be one of the benefits gained from applying biochar to soil
The biosynthesis of 5-aminolevulinate from glutamate in the blue-green alga Spirulina platensis
Isolation and Identification of Fusarium spp., the Causal Agents of Onion (Allium cepa) Basal Rot in Northeastern Israel
Over the past decade, there have been accumulating reports from farmers and field extension personnel on the increasing incidence and spread of onion (Allium cepa) bulb basal rot in northern Israel. The disease is caused mainly by Fusarium species. Rotting onion bulbs were sampled from fields in the Golan Heights in northeastern Israel during the summers of 2017 and 2018. Tissue from the sampled onion bulbs was used for the isolation and identification of the infecting fungal species using colony and microscopic morphology characterization. Final confirmation of the pathogens was performed with PCR amplification and sequencing using fungi-specific and Fusarium species-specific primers. Four Fusarium spp. isolates were identified in onion bulbs samples collected from the contaminated field: F. proliferatum, F. oxysporum f. sp. cepae, and two species less familiar as causative agents of this disease, F. acutatum and F. anthophilium. Phylogenetic analysis revealed that these species subdivided into two populations, a northern group isolated from white (Riverside cv.) onion bulbs, and a southern group isolated from red (565/505 cv.) bulbs. Pathogenicity tests conducted with seedlings and bulbs under moist conditions proved that all species could cause the disease symptoms, but with different degrees of virulence. Inoculating seeds with spore suspensions of the four species, in vitro, significantly reduced seedlings’ germination rate, hypocotyl elongation, and fresh biomass. Mature onion bulbs infected with the fungal isolates produced typical rot symptoms 14 days post-inoculation, and the fungus from each infected bulb was re-isolated and identified to satisfy Koch’s postulates. The onion bulb assay also reflected the degree of sensitivity of different onion cultivars to the disease. This work is the first confirmed report of the direct and primary cause of Fusarium onion basal rot disease in northeastern Israel. These findings are a necessary step towards uncovering the mycoflora of the diseased onion plants and developing a preventive program that would reduce the disease damage