15 research outputs found
Π‘ΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½Π°Ρ ΠΎΡΠ΅Π½ΠΊΠ° Π²Π»ΠΈΡΠ½ΠΈΡ ΡΠΎΡΠ΅ΡΠ°Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Π€Π΅Π»ΠΈΡΠ΅ΡΠΎΠ½ ΠΈ ΠΠ·ΠΎΠΊΡΠΈΠ²Π΅Ρ ΠΈ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° ΠΠ»ΠΎΠ±ΡΠ΅Π»-4 ΠΏΡΠΈ ΡΠΈΠ½ΠΎΡΡΠ°Ρ Π΅ΠΈΡΠ΅ ΠΊΠΎΡΠ΅ΠΊ Π½Π° Π³Π΅ΠΌΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ Π±Π΅Π»ΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ
The comparative effect of the combined drugs Feliferon and Azoksivet and the drug Globfel-4 for rhinotracheitis in felines on hematological parameters of white blood was studied. In our study on the treatment of viral rhinotracheitis, 16 cats were examined. During the experiment, all cats showed the following clinical signs: lethargy, fever, leakage from the eyes and nasal passages. The animals were kept in separate cages in accordance with the European Convention for the Protection of Vertebrates. The control group was given 1 ml subcutaneously, once a day every other day, with a course of 7 injections, immunoglobulin against rhinotracheitis, calicivirus and chlamydia - Globfel-4. The experimental group was treated with drugs Azoxivet intramuscularly in a course of 7 injections, once a day every other day, and Feliferon at a dose of 400,000 IU intramuscularly, once a day. The course of treatment was 7 days. Blood sampling was performed from the anterior saphenous vein of the forearm before and after therapy. The concentration of erythrocytes and hemoglobin was determined on an automatic hematology analyzer Mindray BC-2800. When treating cats with rhinotracheitis with Globfel-4, pronounced leukocytosis, absolute and relative lymphocytosis were noted. With the combined use of Azoxivet and Feliferon, these blood changes do not occur. When treating cats with rhinotracheitis with Globfel-4 or the combined use of Azoxivet and Feliferon, the absolute concentrations of monocytes and granulocytes increase within the physiological norm. The relative concentrations of these white blood phrases increase within the physiological norm only with the combined use of Azoxivet and Feliferon.ΠΠ·ΡΡΠ°Π»ΠΎΡΡ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΡΠΎΡΠ΅ΡΠ°Π½Π½ΠΎΠ³ΠΎ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² Π€Π΅Π»ΠΈΡΠ΅ΡΠΎΠ½ ΠΈ ΠΠ·ΠΎΠΊΡΠΈΠ²Π΅Ρ ΠΈ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° ΠΠ»ΠΎΠ±ΡΠ΅Π»-4 ΠΏΡΠΈ ΡΠΈΠ½ΠΎΡΡΠ°Ρ
Π΅ΠΈΡΠ΅ ΠΊΠΎΡΠ΅ΠΊ Π½Π° Π³Π΅ΠΌΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ Π±Π΅Π»ΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ. Π Π½Π°ΡΠ΅ΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΠΏΠΎ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π»Π΅ΡΠ΅Π½ΠΈΡ Π²ΠΈΡΡΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠ½ΠΎΡΡΠ°Ρ
Π΅ΠΈΡΠ° Π±ΡΠ»ΠΎ ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΎ 16 ΠΊΠΎΡΠ΅ΠΊ. Π£ Π²ΡΠ΅Ρ
ΠΊΠΎΡΠ΅ΠΊ Π² ΠΏΠ΅ΡΠΈΠΎΠ΄ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ ΠΎΠΏΡΡΠ° Π²ΡΡΠ²Π»Π΅Π½Ρ ΡΠ»Π΅Π΄ΡΡΡΠΈΠ΅ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΈ: Π²ΡΠ»ΠΎΡΡΡ, ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΡ, ΠΈΡΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΈΠ· Π³Π»Π°Π· ΠΈ Π½ΠΎΡΠΎΠ²ΡΡ
ΠΏΡΡΠ΅ΠΉ. ΠΠΈΠ²ΠΎΡΠ½ΡΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π»ΠΈΡΡ Π² ΠΎΡΠ΄Π΅Π»ΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΊΠ°Ρ
Π² ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΈΠΈ Ρ ΠΠ²ΡΠΎΠΏΠ΅ΠΉΡΠΊΠΎΠΉ ΠΊΠΎΠ½Π²Π΅Π½ΡΠΈΠ΅ΠΉ ΠΏΠΎ Π·Π°ΡΠΈΡΠ΅ ΠΏΠΎΠ·Π²ΠΎΠ½ΠΎΡΠ½ΡΡ
. ΠΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΠ΅ ΠΏΠΎΡΡΠ°Π²ΠΈΠ»ΠΈ ΠΏΠΎΠ΄ΠΊΠΎΠΆΠ½ΠΎ ΠΏΠΎ 1 ΠΌΠ», 1 ΡΠ°Π· Π² ΡΡΡΠΊΠΈ ΡΠ΅ΡΠ΅Π· Π΄Π΅Π½Ρ, ΠΊΡΡΡΠΎΠΌ 7 Π²Π²Π΅Π΄Π΅Π½ΠΈΠΉ, ΠΈΠΌΠΌΡΠ½ΠΎΠ³Π»ΠΎΠ±ΡΠ»ΠΈΠ½ ΠΏΡΠΎΡΠΈΠ² ΡΠΈΠ½ΠΎΡΡΠ°Ρ
Π΅ΠΈΡΠ°, ΠΊΠ°Π»ΠΈΡΠΈΠ²ΠΈΡΠΎΠ·Π° ΠΈ Ρ
Π»Π°ΠΌΠΈΠ΄ΠΈΠΎΠ·Π° β ΠΠ»ΠΎΠ±ΡΠ΅Π»-4. ΠΠΏΡΡΠ½ΡΡ Π³ΡΡΠΏΠΏΡ Π»Π΅ΡΠΈΠ»ΠΈ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ°ΠΌΠΈ ΠΠ·ΠΎΠΊΡΠΈΠ²Π΅Ρ β Π²Π½ΡΡΡΠΈΠΌΡΡΠ΅ΡΠ½ΠΎ ΠΊΡΡΡΠΎΠΌ 7 Π²Π²Π΅Π΄Π΅Π½ΠΈΠΉ, 1 ΡΠ°Π· Π² ΡΡΡΠΊΠΈ ΡΠ΅ΡΠ΅Π· Π΄Π΅Π½Ρ, ΠΈ Π€Π΅Π»ΠΈΡΠ΅ΡΠΎΠ½ Π² Π΄ΠΎΠ·Π΅ 400 000 ΠΠ Π²Π½ΡΡΡΠΈΠΌΡΡΠ΅ΡΠ½ΠΎ, 1 ΡΠ°Π· Π² Π΄Π΅Π½Ρ, ΠΊΡΡΡ Π»Π΅ΡΠ΅Π½ΠΈΡ 7 Π΄Π½Π΅ΠΉ. ΠΠ°Π±ΠΎΡ ΠΊΡΠΎΠ²ΠΈ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΈΠ· ΠΏΠ΅ΡΠ΅Π΄Π½Π΅ΠΉ ΠΏΠΎΠ΄ΠΊΠΎΠΆΠ½ΠΎΠΉ Π²Π΅Π½Ρ ΠΏΡΠ΅Π΄ΠΏΠ»Π΅ΡΡΡ Π΄ΠΎ Π½Π°ΡΠ°Π»Π° ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΈ ΠΏΠΎΡΠ»Π΅. ΠΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡ ΡΡΠΈΡΡΠΎΡΠΈΡΠΎΠ² ΠΈ Π³Π΅ΠΌΠΎΠ³Π»ΠΎΠ±ΠΈΠ½Π° ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Π½Π° Π³Π΅ΠΌΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌ Π°Π²ΡΠΎΠΌΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΌ Π°Π½Π°Π»ΠΈΠ·Π°ΡΠΎΡΠ΅ Mindray BC-2800. ΠΡΠΈ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΊΠΎΡΠ΅ΠΊ Ρ ΡΠΈΠ½ΠΎΡΡΠ°Ρ
Π΅ΠΈΡΠΎΠΌ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠΌ ΠΠ»ΠΎΠ±ΡΠ΅Π»-4 ΠΎΡΠΌΠ΅ΡΠ°Π΅ΡΡΡ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΡΠΉ Π»Π΅ΠΉΠΊΠΎΡΠΈΡΠΎΠ·, Π°Π±ΡΠΎΠ»ΡΡΠ½ΡΠΉ ΠΈ ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΡΠΉ Π»ΠΈΠΌΡΠΎΡΠΈΡΠΎΠ·. ΠΡΠΈ ΡΠΎΡΠ΅ΡΠ°Π½Π½ΠΎΠΌ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠΈ ΠΠ·ΠΎΠΊΡΠΈΠ²Π΅ΡΠ° ΠΈ Π€Π΅Π»ΠΈΡΠ΅ΡΠΎΠ½Π° Π΄Π°Π½Π½ΡΡ
ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ ΠΊΡΠΎΠ²ΠΈ Π½Π΅ ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΠΈΡ. ΠΡΠΈ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΊΠΎΡΠ΅ΠΊ Ρ ΡΠΈΠ½ΠΎΡΡΠ°Ρ
Π΅ΠΈΡΠΎΠΌ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠΌ ΠΠ»ΠΎΠ±ΡΠ΅Π»-4 ΠΈΠ»ΠΈ ΡΠΎΡΠ΅ΡΠ°Π½Π½ΡΠΌ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² ΠΠ·ΠΎΠΊΡΠΈΠ²Π΅Ρ ΠΈ Π€Π΅Π»ΠΈΡΠ΅ΡΠΎΠ½ ΠΏΠΎΠ²ΡΡΠ°ΡΡΡΡ Π² ΠΏΡΠ΅Π΄Π΅Π»Π°Ρ
ΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½ΠΎΡΠΌΡ Π°Π±ΡΠΎΠ»ΡΡΠ½ΡΠ΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΌΠΎΠ½ΠΎΡΠΈΡΠΎΠ² ΠΈ Π³ΡΠ°Π½ΡΠ»ΠΎΡΠΈΡΠΎΠ². ΠΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΡΡΠΈΡ
ΡΡΠ°ΡΠΈΠΉ Π±Π΅Π»ΠΎΠΉ ΠΊΡΠΎΠ²ΠΈ Π²ΠΎΠ·ΡΠ°ΡΡΠ°ΡΡ Π² ΠΏΡΠ΅Π΄Π΅Π»Π°Ρ
ΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½ΠΎΡΠΌΡ ΡΠΎΠ»ΡΠΊΠΎΒ ΠΏΡΠΈ ΡΠΎΡΠ΅ΡΠ°Π½Π½ΠΎΠΌ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠΈ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΎΠ² ΠΠ·ΠΎΠΊΡΠΈΠ²Π΅Ρ ΠΈ Π€Π΅Π»ΠΈΡΠ΅ΡΠΎΠ½
Influence of long-term antibiotic therapy on gut microbiome composition and metabolic profile in pulmonary tuberculosis
The use of long-term multicomponent antibiotic therapy is the most effective way to treat tuberculosis (TB). However, little is known about the effect of this chemotherapy on the human intestinal microflora. The purpose of this study was to analyze an effect of long-term antibiotic therapy on gut microbiome composition and metabolic profile in TB patients. We used deep sequencing of fecal samples from 23 treatment-naive TB patients to reconstruct the metabolic capacity and strain/species-level abundance in the gut microbiome. Two fecal samples were obtained from each patient: before and after treatment. We showed that TB treatment regimen does not disrupt the overall diversity of the gut microbiome but does have an impact on gut bacterial microbiome composition and metabolic profile. While taking first-line anti-tuberculosis drugs (isoniazid, rifampicin, ethambutol, pyrazinamide), TB patients showed an apparent increase in Actinobacteria abundance. Pairwise comparison of metagenomic data revealed 28 differentially represented bacterial taxa, of which three species Bacteroides cellulosilyticus, Enterocloster aldensis, Clostridium spiroforme were strongly enriched in TB patients post-chemotherapy, whereas 25 species were enriched in TB patients before treatment (Bifidobacterium catenulatum, Enterococcus faecium, Bacteroides salyersiae, Bacteroides xylanisolvens, Bacteroides eggerthii, Lachnospira eligens, Akkermansia muciniphila, Ruminococcus lactaris, etc.) (p 0.05). The metabolic profile of the gut microbiome was characterized by increased metabolic processes aimed at the growth and division of microbial cells. Iron is the main limiting factor for growth and reproduction. In addition, it is important to note the prevalence of glycolysis and lactate fermentation as the major means for energy production by intestinal microbiota