Desorption of cadmium from a natural Shanghai clay using citric acid industrial wastewater

The sorption/desorption characteristics of heavy metals onto/from soil particle surfaces are the primary factors controlling the success of the remediation of heavy-metal contaminated soils. These characteristics are pH-dependent, chemical-specific, and reversible; and can be modified by enhancement agents such as chelates and surfactants. In this study, batch experiments were conducted to evaluate the feasibility of using citric acid industrial wastewater (CAIW) to desorb cadmium from a natural clay from Shanghai, China at different soil mixture pHs. It can be observed from the results that the proportion of cadmium desorbed from the soil using synthesized CAIW is generally satisfactory, i.e., >60%, when the soil mixture pH is lower than 6. However, the proportion of desorbed cadmium decreases significantly with increase in soil mixture pH. The dominant cadmium desorption mechanism using CAIW is the complexion of cadmium with citric acid and acetic acid in CAIW. It is concluded that CAIW can be a promising enhancement agent for the remediation of cadmium-contaminated natural soils when the environmental conditions are favorable. As a result, CAIW, a waste product itself, can be put into productive use in soil remediation. © 2011 Elsevier B.V.postprin

我愛長腿叔叔 - 文字輔導對兒童成長的意義

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Combination of SAHA and bortezomib up-regulates CDKN2A and CDKN1A and induces apoptosis of Epstein-Barr virus-positive Wp-restricted Burkitt lymphoma and lymphoblastoid cell lines

Epstein-Barr virus (EBV) latent proteins exert anti-apoptotic effects on EBV-transformed lymphoid cells by down-regulating BCL2L11 (BIM), CDKN2A (p16(INK4A) ) and CDKN1A (p21(WAF1) ). However, the potential therapeutic effects of targeting these anti-apoptotic mechanisms remain unexplored. Here, we tested both in vitro and in vivo effects of the combination of histone deacetylase (HDAC) and proteasome inhibitors on the apoptosis of six endemic Burkitt lymphoma (BL) lines of different latency patterns (types I and III and Wp-restricted) and three lymphoblastoid cell lines (LCLs). We found that the combination of HDAC and proteasome inhibitors (e.g. SAHA/bortezomib) synergistically induced the killing of Wp-restricted and latency III BL and LCLs but not latency I BL cells. The synergistic killing was due to apoptosis, as evidenced by the high percentage of annexin V positivity and strong cleavage of PARP1 (PARP) and CASP3 (caspase-3). Concomitantly, SAHA/bortezomib up-regulated the expression of CDKN2A and CDKN1A but did not affect the level of BCL2L11 or BHRF1 (viral homologue of BCL2). The apoptotic effects were dependent on reactive oxygen species generation. Furthermore, SAHA/bortezomib suppressed the growth of Wp-restricted BL xenografts in nude mice. This study provides the rationale to test the novel application of SAHA/bortezomib on the treatment of EBV-associated Wp-restricted BL and post-transplant lymphoproliferative disorder.postprin

Macrophages promoted the colony forming ability of putative endometrial stromal stem cells

Poster Session - Blood Disorders & Stem Cell Immunology: no. 69DMM 2011 entitled: Re-engineering Regenerative MedicineWomen with endometriosis have a decreased cell-mediated immunity1 and contain more activated macrophages2. Human endometrial and endometriotic stem/progenitor cells have been identified using the clonogenic assay3, 4 ENREF 4 ENREF 4. Large colony forming units (CFUs, >4000 cells) are initiated from stem/progenitor cells and small CFUs (<4000 cells) are from transit-amplifying cells. Retrograded endometrial stem cells may have a role in the pathogenesis of endometriosis. In this study, the regulatory mechanism between macrophages and putative stem cells was examined. Endometrium (n=12)/ovarian endometrioma (n=16) were obtained from women undergoing hysterectomy and …postprin

The role of SPECT bone scan in unilateral condylar hyperplasia: is it measuring the condyle size?

Oral Presentations: Session 40 - Temporomandibular Joint Pathology and Surgery II: O-4007BACKGROUND: The use of Tc-99m SPECT bone scan has been recommended as the assessment tool for patients with unilateral condylar hyperplasia. A condyle is generally regarded as active if the scintigraphic activity difference is 10% greater than the contralateral side. However, there was little information regarding the association between the bone scan uptake and the histopathological features of the hyperactive condyle. AIM: The aim of this study is to check the association between the SPECT bone scan uptake and the dimension of the mandibular condyle in patients with mandibular asymmetry caused by condylar hyperplasia. MATERIAL AND METHOD: 27 patients with mandibular asymmetry caused by condylar hyperplasia were recruited. The clinical records, dental casts, SPECT and cone-beam CT data were used for the analysis. The clinical records and serial dental casts were used to check the activity of asymmetry . The cone-beam CT data were imported for building the virtual mandibular model for the 3-dimensional analysis of the hyperactive and contralateral condyles. 3-D cephalometric analysis was performed to measure the linear dimension of condyle and ramus. Pearson correlation analysis was used to check the association of the percentile difference between the SPECT scan and (i) condylar dimensions including x, y and z-axis, (ii) dimensions of mandibular ramus and body. RESULT: Among the 27 patients, only 2 patients showed active asymmetry from clinical and model analysis plus cone beam CT superimposition. The mean percentile difference between the right and left condyles in the remaining 25 patients showing inactive asymmetry was 12.05%. 12 patients (48%) having percentile differences > 10 %. Correlation analysis revealed linear association in the percentile difference of condyles between the SPECT scan uptake and (i) the linear dimensions of the condyle (R ranged from 0.68-0.83), (ii) surface area of the condyle (R=0.80), (iii) vertical ramus height (R=0.81) and (iv) mandibular body length (R=0.85). CONCLUSIONS: There was strong association between the condyle dimension and the SPECT uptake. Patients with inactive mandibular asymmetry caused by condylar hyperplasia could have the percentile difference greater than 10%.postprin

BCR-ABL mutational studies for predicting the response of patients with chronic myeloid leukaemia to second-generation tyrosine kinase inhibitors after imatinib fail

Imatinib is the standard treatment for chronic myeloid leukaemia. BCR-ABL kinase domain mutation is the commonest mechanism implicated in imatinib resistance. In in-vitro studies, kinase domain mutations are variably resistant to second-line agents. We performed BCR-ABL kinase domain mutational studies in 25 patients in five institutions who failed imatinib and were treated with either nilotinib or dasatinib, to see if their mutational status would predict their clinical responses. Kinase domain mutations involving 11 amino acid substitutions were found in 12 (48%) patients. Most patients showed single kinase domain mutations. There was some concordance between reported drug sensitivity patterns and patient responses. Discordant responses could be related to drug dosage variations and unknown BCR-ABL independent mechanisms. The response prediction for patients with multiple kinase domain mutations was challenging and their mutational patterns could change after tyrosine kinase inhibitor therapy. Although BCR-ABL kinase domain mutational analysis has limitations as a means of predicting the clinical response to second-line tyrosine kinase inhibitors, it helps inform therapy decisions in the management of chronic myeloid leukaemia after imatinib failure.published_or_final_versio

Early-phase circulating miRNAs predict tumor recurrence and survival of hepatocellular carcinoma patients after liver transplantation

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The clinical significance and potential therapeutic role of GPx3 in tumor recurrence after liver transplantation

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Clinical significance and therapeutic value of glutathione peroxidase 3 (GPx3) in hepatocellular carcinoma

AIMS: We aimed to investigate the clinical significance of GPx3 in hepatocellular carcinoma (HCC) and to characterize its tumor suppressive role. METHODS: HCC patients (113) who underwent hepatectomy were recruited to examine the clinical relevance of GPx3. The tumor suppressive role of GPx3 was studied by administration of recombinant GPx3 (rGPx3) or over-expression of GPx3 in HCC cells in vitro and in vivo. The therapeutic value of GPx3 for HCC was further investigated using human induced pluripotent stem cell derived mesenchymal stem cells (hiPSC-MSCs) as its delivery vehicle. RESULTS: Down-regulation of GPx3 significantly correlated with advanced tumor stage (P = 0.024), venous infiltration (P = 0.043) and poor overall survival (P = 0.007) after hepatectomy. Lower plasma GPx3 in HCC patients was significantly associated with larger tumor size (P = 0.011), more tumor nodules (P = 0.032) and higher recurrence (P = 0.016). Over-expression of GPx3 or administration of rGPx3 significantly inhibited proliferation and invasiveness of HCC cells in vitro and in vivo. Tumor suppressive activity of GPx3 was mediated through Erk-NFκB-SIP1 pathway. GPx3 could be delivered by hiPSC-MSCs into the tumor and exhibited tumor suppressive activity in vivo. CONCLUSIONS: GPx3 is a tumor suppressor gene in HCC and may possess prognostic and therapeutic value for HCC patients.published_or_final_versio

CXCL10/CXCR3 signaling mobilized-regulatory T cells promote liver tumor recurrence after transplantation

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