2 research outputs found
Fucoidan-degrading fungal strains: screening, morphometric evaluation, and influence of medium composition
Ten different fungal strains from the genus Aspergillus, Penicillium, and Mucor
were screened for fucoidan hydrolyzing ability aiming to find microorganisms able to
produce sulfated fucan-degrading enzymes. Screening was carried out by measuring the
strains kinetic and morphometric behavior over plate assays using Laminaria japonica
fucoidan as only carbon source, testing three nitrogen sources (urea, peptone, and sodium
nitrate). The selected fungal strains were subsequently used in submerged fermentations,
which were performed for (1) selection of the strains able to growth over fucoidan medium
and (2) media selection, testing the synergy of fucoidan with other sugars for inducing high
enzyme titles. Radial expansion and hyphae parameters were observed for Aspergillus niger
PSH, Mucor sp. 3P, and Penicillium purpurogenum GH2 grown only over fucoidan-urea
medium. A. niger PSH showed the maximum enzymatic activity values, which were
significantly different (p<0.05) from those achieved by the other selected fungi. Sucrose
addition to fucoidan media proportioned the highest fucoidanase activity values for this
fungal strain. This research allowed establishing optimal conditions for metabolites
synthesis by fungal stains able to act toward fucoidan ramified matrix.Mexican
Science and Technology Council (CONACYT