55 research outputs found
Analyses on Stopping-off Points and Destination Selection of Nagoya CBD Visitors by applying Spatial-Temporal Position Data
In recent years, GPS(Global Positioning System) data and various other data have been provided as
information on people's shopping-around behaviors within CBD(Central Business District), allowing
people's shopping-around behaviors to be obtained in more detail. In addition to conventional person-trip
surveys, these data have the potential to reveal new aspects of people's shopping-around behaviors.
Furthermore, through the restaurant API(Application Programming Interface), by combining spatialtemporal position data with restaurant data, it will be possible to clarify people's district visitation factors and
downtown selection behavior.
In this study, We defined a 5-minute stop in the area as a stopping off points in the spatial-temporal position
data, and aimed to clarify the factors of stopping off points in the Nagoya CBD area by latent factor analysis
of PLS(Partial Least Squares) regression, and to estimate individual destination selection behavior by
multinomial logistic analysis. The results of the latent factor analysis of the PLS regression revealed that the
regression coefficients were high for the number and type of restaurants in the 0-12 time period, but high for
entertainment facilities in the 12-18 and 18-24 time periods. Furthermore, multinomial logistic analysis
revealed a trade-off between proximity of 3 meters and the utility obtained from one restaurant
Progress of the IUGONET system - metadata database for upper atmosphere ground-based observation data
Development of metadata database and analysis software for upper atmospheric researches by the IUGONET project
第2回極域科学シンポジウム/第35回極域宙空圏シンポジウム 11月15日(火) 国立極地研究所 2階大会議室前フロ
Quantitative Determination of Growth of Amastigotes and Trypomastigotes in an In Vitro Cultivation System of HeLa Cells Infected with Trypanosoma cruzi
VH3 Gene Usage in Neutralizing Human Antibodies Specific for the Entamoeba histolytica Gal/GalNAc Lectin Heavy Subunit
A combinatorial human immunoglobulin gene library was constructed from peripheral lymphocytes of an asymptomatic Entamoeba histolytica cyst passer and screened for the production of Fab antibody to the parasite. One of the Fab clones, CP33, recognized the 260-kDa galactose- and N-acetyl-d-galactosamine (Gal/GalNAc)-specific lectin of E. histolytica. By shuffling the heavy and light chains of CP33 with the heavy and light chains of two libraries derived from the cyst passer and a liver abscess patient, 18 additional clones were obtained. Sequence analysis of the heavy-chain genes, including CP33-H, revealed that all the nearest V-segment germ lines belonged to the VH3 family (VH3-21, VH3-30, VH3-48, and VH3-53), but the levels of homology were only 85 to 95%. The closest D-segment germ line was D2-2 or D6-6, and for the J-segment the closest germ line was JH4b or JH6b. On the other hand, all the light-chain genes, including CP33-L, belonged to the Vκ1 family, in which the closest Vκ germ line gene was 02/012 or L5, with the Jκ1, Jκ2, Jκ4, or Jκ5 segment. CP33 and three other Fabs obtained by light-chain shuffling were purified and analyzed further. All of these Fabs recognized the cysteine-rich domain of the 170-kDa heavy subunit of the Gal/GalNAc lectin. Preincubation of E. histolytica trophozoites with these Fabs significantly inhibited amebic adherence to Chinese hamster ovary cells and also inhibited erythrophagocytosis. The ability of the neutralizing antibodies to block erythrophagocytosis for the first time implicates the lectin in phagocytosis and VH3 antibodies in defense against parasitic infections. These results demonstrate the utility of a combinatorial human immunoglobulin gene library for identifying and characterizing neutralizing antibodies from humans with amebiasis
Distribution of polar upper atmospheric data and promotion of polar science by the IUGONET project
極地研研究集会「極域観測研究の過去・現在・未来」, 2012年2月17
- …