A two-step sensitivity analysis for hydrological signatures in Jinhua River Basin, East China

This is the author accepted manuscript. The final version is available from Taylor & Francis via the DOI in this record.Parameter calibration and sensitivity analysis are usually not straightforward tasks for distributed hydrological models, owing to the complexity of model and large number of parameters. A two-step sensitivity analysis approach is proposed for analyzing the hydrological signatures based on the Distributed Hydrology-Soil-Vegetation Model in Jinhua River Basin, East China. A preliminary sensitivity analysis is conducted to obtain influential parameters via Analysis of Variance. These parameters are further analyzed through a variance-based global sensitivity analysis method to achieve robust rankings and parameter contributions. Parallel computing is designed to reduce computational burden. The results reveal that only a few parameters are significantly sensitive and the interactions between parameters could not be ignored. When analyzing hydrological signatures, it is found that water yield was simulated very well for most samples. Small and medium floods are simulated very well while slight underestimations happen to large floods.This work was supported by National Natural Science Foundation of China (91547106 and 51379183), Zhejiang Provincial Natural Science Foundation of China (LR14E090001), and National Key Research and Development Plan "Inter-governmental Cooperation in International Scientific and Technological Innovation"(2016YFE0122100)

Adipose tissue-derived stem cells in oral mucosa tissue engineering: Enhanced migration and proliferation in co-culture with oral keratinocytes in vitro

Tissue-engineered oral mucosa holds a great prospect in urethroplasty and adipose tissue-derived stem cells (ADSCs) may play an important role in this field. In this research, canine oral keratinocytes (OKs) and ADSCs were harvested and cultured in vitro. The affinity between the two cell lines was evaluated by analyzing their migration and proliferation patterns in a co-culture environment. The results demonstrate that both canine ADSCs and OKs showed improved migration in the presence of the other cell line as a co-culture when compared to monoculture. Further, conditioned medium using the supernatant of one cell line accelerated the other cell line’s proliferation rate. Hence, it was concluded that the affinity between OKs and ADSCs was fitting; the presence of ADSCs accelerated the migration and proliferation of OKs in vitro. These results indicate that it is practical to use ADSCs and OKs to construct a tissue-engineered oral mucosa, since the presence of the former could activate the latter in vitro, maybe even in vivo. This may help to build tissue-engineered oral mucosa, which may be a new method for urethroplasty.Key words: Urethroplasty, adipose tissue-derived stem cells, oral keratinocytes, tissue engineering

PIKfyve, a class III lipid kinase, is required for TLR-induced type I IFN production via modulation of ATF3

Type I IFN plays a key role in antiviral responses. It also has been shown that deregulation of type I IFN expression following abnormal activation of TLRs contributes to the pathogenesis of systemic lupus erythematosus. In this study, we find that PIKfyve, a class III lipid kinase, is required for endolysosomal TLR-induced expression of type I IFN in mouse and human cells. PIKfyve binds to phosphatidylinositol 3-phosphate and synthesizes phosphatidylinositol 3,5-bisphosphate, and plays a critical role in endolysosomal trafficking. However, PIKfyve modulates type I IFN production via mechanisms independent of receptor and ligand trafficking in endolysosomes. Instead, pharmacological or genetic inactivation of PIKfyve rapidly induces expression of the transcription repressor ATF3, which is necessary and sufficient for suppression of type I IFN expression by bin'ding to its promoter and blocking its transcription. Thus, we have uncovered a novel phosphoinositide-mediated regulatory mechanism that controls TLR-mediated induction of type I IFN, which may provide a new therapeutic indication for the PIKfyve inhibitor.X111313Ysciescopu

The effect of Ga-doped nanocrystalline ZnO electrode on deep-ultraviolet enhanced GaN photodetector

published_or_final_versio

Evaluation of Chemical Constituents and Important Mechanism of Pharmacological Biology in Dendrobium Plants

published_or_final_versio

Effects of annealing temperature on the characteristics of Ga-doped ZnO film metal-semiconductor-metal ultraviolet photodetectors

published_or_final_versio

Initial validation of Chinese Pain Assessment in Advanced Dementia Scale (C-PAINAD)

2007-2008 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells

Protein array technology is a powerful platform for the simultaneous determination of the expression levels of a number of proteins as well as post-translational modifications such as phosphorylation. Here, we screen and report for the first time, the dominant signaling cascades and apoptotic mediators during the course of cadmium (Cd)-induced cytotoxicity in human bronchial epithelial cells (BEAS-2B) by antibody array analyses. Proteins from control and Cd-treated cells were captured on Proteome Profiler™ Arrays for the parallel determination of the relative levels of protein phosphorylation and proteins associated with apoptosis. Our results indicated that the p38 MAPK- and JNK-related signal transduction pathways were dramatically activated by Cd treatment. Cd potently stimulates the phosphorylations of p38α (MAPK14), JNK1/2 (MAPK8/9), and JUN; while the phosphorylations of Akt1, ERK1/2 (MAPK3/1), GSK3β, and mTOR were suppressed. Moreover, there was an induction of proapoptotic protein BAX, release of cytochrome c (CYCS) from mitochondria, activation of caspase-3/9 (CASP3/9); as well as decreased expression of cell cycle checkpoint proteins (TP53, p21, and p27) and several inhibitors of apoptosis proteins (IAPs) [including cIAP-1/2 (BIRC2/3), XIAP (BIRC4), and survivin (BIRC5)]. Pretreatment of cells with the thiol antioxidant glutathione or p38 MAPK/JNK inhibitors before Cd treatment effectively abrogated ROS activation of p38 MAPK/JNK pathways and apoptosis-related proteins. Taken together, our results demonstrate that Cd causes oxidative stress-induced apoptosis; and the p38 MAPK/JNK and mitochondrial pathways are more importantly participated for signal transduction and the induction of apoptosis in Cd-exposed human lung cells.published_or_final_versio

Wideband Diplexer with Narrow Channel Spacing Using Hybrid Bandpass-Bandstop Structures

© 2013 IEEE. In this paper, a wideband diplexer with narrow channel spacing is designed by using hybrid bandpass-bandstop structures. Two wideband bandpass structures are designed with the transmission zeros at the upper or lower passband edges to achieve high skirt selectivity. Two bandstop structures based on half-wavelength coupled lines are integrated to the two bandpass structures to introduce additional transmission zeros and thus the skirt selectivity is further improved. Accordingly, two channel filters with high skirt selectivity and high stopband rejection are designed to realize a wideband microstrip diplexer with very narrow channel spacing. For verification, a wideband diplexer operating at 1.71-2.17 GHz and 2.30-2.70 GHz is implemented, which covers multiple frequency bands for different mobile systems. The measured results show excellent performance of passband flatness, high in-band isolation of better than 35 dB and low minimum insertion losses of 0.46 and 0.50 dB for the two channels