Protein Functionalized Nanodiamond Arrays

Various nanoscale elements are currently being explored for bio-applications, such as in bio-images, bio-detection, and bio-sensors. Among them, nanodiamonds possess remarkable features such as low bio-cytotoxicity, good optical property in fluorescent and Raman spectra, and good photostability for bio-applications. In this work, we devise techniques to position functionalized nanodiamonds on self-assembled monolayer (SAMs) arrays adsorbed on silicon and ITO substrates surface using electron beam lithography techniques. The nanodiamond arrays were functionalized with lysozyme to target a certain biomolecule or protein specifically. The optical properties of the nanodiamond-protein complex arrays were characterized by a high throughput confocal microscope. The synthesized nanodiamond-lysozyme complex arrays were found to still retain their functionality in interacting with E. coli

Amphiphilic Protein Microfibrils from Ice-Templated Self-Assembly and Disassembly of Pickering Emulsions

Amphiphilic protein microfibrils have been generated for the first time by ice-templated self-assembly of aqueous globular protein colloids and subsequent selective disassembly in polar solvents like MeOH, EtOH, acetone, and dimethylformamide. Semicrystalline microfibrils, ca. 1.2 μm wide and 45-70 μm long, produced from soy proteins are excellent amphiphiles, which are capable of stabilizing both high-internal-phase o/w and w1/o/w2 double emulsions as well as retaining amphiphilicity even with surface-bound lipophiles and electrophiles. This ice-templated self-assembling and polar solvent disassembling approach is applicable to other legume proteins, such as pea proteins, and is scalable to process globular proteins into amphiphilic microfibrils for Pickering emulsions in many potential applications including food, pharmaceuticals and skin care

Tolerance associated with cord blood transplantation may depend on the state of host dentritic cells

Allogeneic cord blood (CB) transplantation is associated with less severe graft-versus-host disease (GvHD), thought to be due to the immaturity of CB T cells, but how T cells interact with host and donor-derived dendritic cells (DCs) to initiate GvHD has not been elucidated. We therefore investigated the responses of CB and adult blood CD4 + T cells co-cultured with adult host DCs of different maturities. Primed by adult host DCs, CB and adult blood CD4 + T cells underwent similar changes in the expression of CD45RA/45RO, CD25, CD40L and CTLA-4. However, CB CD4 + T cells, when primed by either immature or Bacillus Calmette-Guerin mycobacteria-treated adult host DCs, produced lower interferon-γ (IFN-γ) and higher interleukin-10 (IL-10), which is a regulatory T cell-like cytokine profile, as compared with adult blood CD4 + T cells. In contrast, lipopolysaccharide (LPS)-treated adult host DCs significantly up-regulated IFN-γ and down-regulated IL-10 production levels from CB CD4 + T cells to that from adult blood CD4 + T cells. The sustained low IFN-γ and high IL-10 production from CB CD4 + T cells co-cultured with adult blood DCs might account for the less severe GvHD occurrence after CB transplantation, which could be reversed by LPS-treated adult blood DCs.postprin

The inversion of anelastic coefficient, source parameters and site respond using genetic algorithm

2002-2003 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Experimental confirmation of frequency correlation for bifurcation in current-mode controlled buck-boost converters

Author name used in this publication: Ka Wai Eric Cheng2003-2004 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Sub-6GHz 4G/5G Conformal Glasses Antennas

© 2013 IEEE. The difficulty of antenna design applied to glasses is that the structure of glasses is too single, and the space available for antenna design is greatly limited. In this background, the integrated design of 4G antennas and 5G antennas applied to glasses is proposed in this paper. The most important highlight of this design is that it makes full use of the limited three-dimensional space structure provided by glasses and achieves the perfect combination of the antenna and glasses in the physical structure. Specifically, two antennas for 4G communication are arranged on two glasses frames, and four antennas for 5G communication are arranged on two glasses legs. In this way, we can make full use of the space provided by the glasses to design antennas and ensure that there is a certain distance between the 4G antennas and 5G antennas so that the performance of both 4G antennas and 5G antennas can be guaranteed. The 4G antenna consists of a loop structure printed on the frame and leg of the glasses and a parasitic branch strip printed on the front of the leg of the glasses. The resonance modes of the 4G antenna are mainly loop, monopole, and dipole modes, which can cover two 4G bands of 0.824-0.96 GHz and 1.71-2.69 GHz. Each 5G antenna mainly comes from the open slot mode etched on the metal ground surface of an FR4 substrate of the glasses leg. In addition, the slot antennas operate in two 5G bands of 3.3-3.6 GHz and 4.8-5.0 GHz. Finally, the glasses and the antennas are fabricated based on FR4 substrates and measured. The measured results show that the proposed antennas perform well and have the potential to be used in 4G/5G communications through glasses

Studies on the expansion characteristics of the granular bed present in EGSB bioreactors

In this study, the expansion characteristics of an anaerobic granular bed in EGSB reactors based on terminal settling velocity study of the granules and the Richardson-Zaki equation (1954) have been investigated. The settling velocity study shows that the mean settling velocity of the granules is in accordance with the Allen formula because the settling process falls within the intermediate flow regime range (

Passive PT -symmetric couplers without complex optical potentials

© 2015 American Physical Society. In addition to the implementation of parity-time-(PT-) symmetric optical systems by carefully and actively controlling the gain and loss, we show that a 2×2 PT-symmetric Hamiltonian has a unitarily equivalent representation without complex optical potentials in the resulting optical coupler. Through the Naimark dilation in operator algebra, passive PT-symmetric couplers can thus be implemented with a refractive index of real values and asymmetric coupling coefficients. This opens up the possibility to implement general PT-symmetric systems with state-of-the-art asymmetric slab waveguides, dissimilar optical fibers, or cavities with chiral mirrors

Essential Role of NK Cells in IgG Therapy for Experimental Autoimmune Encephalomyelitis

published_or_final_versio