Studies on the Expression of Color Tone in Rose Petals Ⅲ: The Role of Anthocyanin in the Expression of Black Tone in the Petals of Velvety Dark Red Roses.

ArticleJournal of the Faculty of Liberal Arts and Science, Shinshu University. Part 2, Natural science 15: 23-30(1965)departmental bulletin pape

Studies on the Expression of Color Tone in Rose Petals. I : Characteristic Structure of the Epidermis of Petals in Velvety Dark Red Roses.

ArticleJournal of the Faculty of Liberal Arts and Science, Shinshu University. Part 2, Natural science 14: 31-37(1964)departmental bulletin pape

Studies on the Expression of Color Tone in Rose Petals X. : Transmittance Curve of the Red Petal Extract, Measured with Translucent Cuvette.

On the reflectance measurements of red petals, part of incident light should be absorbed into some inner constituents of petals, and the remaining part passed away through the petals. The only part of incident light is detected by the spectrophotometer as the reflected light. In the present study, the transmittance measurements on pigment solutions were taken with the translucent cuvette to give an optical state similar to the translucent property of the petal. The translucent cuvettes were prepared by inserting the semitransparent pieces of plastic sheets along the inner walls of ordinary cuvettes. When the cuvettes having the transmittance of 40 to 60 % were used, the transmittance curves of red petal extracts became more approximate to the reflectance ones of red petals. From the results of colorimetric calculation it is suggested that the translucent property brings some modification of the color tone in the petals having higher content of pigment.Article信州大学理学部紀要 8(2): 127-134(1974)departmental bulletin pape

Studies on Bluing Effect in the Petals of Red Rose, IV. : Calcium in the Blue Spherical Body.

Both the localization of calcium within the blue spherical body presented in the upper epidermal cells, and the role of the element in this body were studied. The upper epidermis of the bluing petals of a rose cultivar, cl-Crimson Glory, was fixed with 10 % neutral buffered formalin, and paraffin sections were prepared. The region corresponding to the central layer of the spherical body was clearly stained red with alizarin red sulfonate under pH 5.5. In the bluing petals treated with EDTA, the central layers of the bodies were not found and the body looked somewhat ring-like. These results show that the calcium localizes within the central body, (a) making a salt or being weakly bound with a tannic substance within the body, and (b) playing a role in making the layer rather rigid.Article信州大学理学部紀要 11(1): 41-46(1976)departmental bulletin pape

A Method for the Quantitative Determination of Anthocyanin in Rose Petals.

ArticleJournal of the Faculty of Liberal Arts and Science, Shinshu University. Part 2, Natural science 14: 39-42(1964)departmental bulletin pape

Studies on the Insoluble States of Anthocyanin in Rose Petals, I. : The Insoluble State of Anthocyanin and its Relationship to Petal Color, together with a new Instance of this Relationship

The upper epidermal cells of certain cultivars of black roses, e.g. Charles Mallerin, Josephine Bruce, Bonne Nuit and so on, occasionally hold in the cell vacuoles massive structures colored purple or purplish-red. It was observed histochemically that these massive structures showed a color change from either purplish-red or from purple to red with dilute hydrochloric acid and to bluish purple with a weak solution of sodium hydroxide. From these results the massive structure found in the red rose petals can be regarded as the insoluble state of anthocyanin. In the surface view of those petals having these massive structures in their upper epidermal cells, the numerous black spots, which are considered to be shadows of epidermal cells, are visible even in petals whose height/width ratio of the upper epidermal cells are within the range of the red petal ones. It can therefore be said that the insoluble state of anthocyanin present in the red rose petals may play an important role in the blackening effect on the petal color.Article信州大学理学部紀要 9(1): 63-69(1974)departmental bulletin pape

Studies on the Expression of Color Tone in Rose Petals. II : Changes of the Epidermal Structure in the Velvety Dark Red Petals as Flower Develops.

ArticleJournal of the Faculty of Liberal Arts and Science, Shinshu University. Part 2, Natural science 15: 15-21(1965)departmental bulletin pape

Epidermal Structure on Coloring in the Leaves of VELVET RAINBOW, a Cloleus

The leaf surface of a coleus, VELVET RAINBOW, has somewhat velvety dark red color like the petal of some black roses. The leaf structure of this cultivar is compared with that of RED RAINBOW, a cultivar not velvety dark red. The results observed here are as follows: (1) No essential difference in the leaf sections between two cultivars is recognized except for their surface structures. (2) In the surface structure the epidermal cells of the leaf of VELVET RAINBOW are of longer papillate form, their lateral walls are less slanting to the surface and the spaces among them are more narrow than in those of RED RAINBOW. (3) In the surface view of the leaf of VELVET RAINBOW the shadow is found on epidermal cell walls, when the surface is illuminated at angles of 25 and 20 degrees, at which the shadow is not visible in that of RED RAINBOW. These facts can lead the author to the conclusion that the blackish appearance on the leaf of Velvet Rainbow is mainly caused by the shadow of epidermal cells, and the surface structure of the leaf of this cultivar may be more suitable to form the shadow.Article信州大学理学部紀要 1(1): 65-70(1967)departmental bulletin pape

Studies on Bluing Effect in the Petals of Red Rose, III. : The Histochemical Detection of Iron in the Bluing Petals of Rose.

The bluing petals of red rose cultivar, cl-Crimson Glory, were fixed with 10% neutral buffered formalin. In the paraffin sections, the region corresponding to the blue spherule present in the upper epidermal cells, was clearly stained blue with the Prussian blue reaction. The same regions showed a brownish red color of ferric oxide with incineration. These facts are suggestive of that iron exists within the blue spherule, making the previous possibility more likely.Article信州大学理学部紀要 8(1): 91-94(1973)departmental bulletin pape

The leucoanthocyanine : reaction in the petals of white garden roses

ArticleJournal of the Faculty of Liberal Arts and Science, Shinshu University. Part 2, Natural science 10: 14-20(1961)departmental bulletin pape