11 research outputs found
Data in support for the measurement of serum 25-hydroxyvitamin D (25OHD) by tandem mass spectrometry
This article provides data and a method related to a research paper entitled âAssessing vitamin D nutritional status: is capillary blood adequate?â (Jensen et al., 2016) [1]. Circulating 25OHD, the accepted biomarker of the vitamin D nutritional status, is routinely measured by automated immunoassays, that although may be performed in hospital central laboratories, often suffer from a lack of specificity with regards to the different vitamin D metabolites, âMeasurement of circulating 25-hydroxyvitamin D: a historical reviewâ (Le Goff et al., 2015) [2]. Mass spectrometry offers this specificity. This article describes the performance of an in-house tandem mass spectrometry method for the individual measurement of 25OHD3, 25OHD2 and 3-Ă©pi-25OHD3. Keywords: Vitamin D, 25-hydroxyvitamin D, Mass spectrometr
Data for the measurement of serum vitamin D metabolites in childhood acute lymphoblastic leukemia survivors
This article describes data related to a companion research paper entitled âVitamin D nutritional status and bone turnover biomarkers in childhood acute lymphoblastic leukemia (cALL) survivors.â (Delvin et al., submitted for publication) [1]. Various methods for the measurement of serum 25OHD3, the accepted biomarker for assessing vitamin D nutritional status, have been described (Le Goff et al., 2015; Jensen et al., 2016) [2,3]. This article describes a novel mass spectrometry-QTOF method for the quantification of circulating 25OHD3, 3-epi-25OHD3 and 24,25(OH)2D3. It provides the description of the extraction, chromatography and mass spectrometry protocols, a sample of mass spectra obtained from standards and extracted serum, and a comparison with another HPLC-MS/MS (Jensen et al., 2016) [3] method for the measurement of serum concentrations of 25OHD3