26 research outputs found
Place et rôle des biotechnologies dans les systèmes de recherche agronomique des pays méditerranéens
Single cross alfalfa (Medicago sativa L.) hybrids produced via 2n gametes and somatic chromosome doubling: experimental and theoretical comparisons
Genetic analysis for haploid-regenerations responses of the hexaploid-wheat anther cultures
Allozyme segregation and inter-cytotype reproductive barriers in the polyploid complex Centaurea jacea.
In eastern Belgium, diploid and tetraploid knapweeds (Centaurea jacea L. s. l.) show a parapatric distribution with a contact zone. To compare the success of inter- and intra-cytotype crosses, and to investigate the pattern of allozyme segregation, controlled crosses involving plants from this contact zone were performed. Polysomic inheritance at two loci was observed in tetraploids, suggesting an autopolyploid origin. Two crosses allowed the detection of double reduction events in tetraploids at one locus, but no such event was detected among the 217 progenies. Null alleles were detected in tetraploids at two loci. Both cytotypes were highly self-incompatible. Inter-cytotype crosses were much less successful than intra-cytotype crosses in terms of seed set (five-fold reduction) and germination rates (three-fold reduction), suggesting a 'triploid block' effect. Progenies from inter-cytotype crosses most often had the maternal cytotype and resulted from selfing or pollen contamination. However, six triploids were detected. The significance of these results for the understanding of the geographical distribution of the cytotypes and inter-cytotype gene flow is discussed.Journal ArticleResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe