14 research outputs found
LâObservatoire agricole de la biodiversitĂ© (OAB) : une pĂ©dagogie active autour dâun projet de sciences participatives
National audiencePlan de l'articleIntroductionLa vocation pĂ©dagogique des sciences participativesLâOAB : principe, objectif, importance des rĂ©seauxPrincipeObjectifsGouvernanceLe rĂ©seauLes premiers rĂ©sultatsLâOAB, un outil de recherche de rĂ©ponses techniques pour les agriculteursLes perspectives et voies dâamĂ©lioration du projet pour rĂ©pondre aux attentes : les sites fixesConclusio
Rapid and simple UPLC-MS/MS method for precise phytochelatin quantification in alga extracts
The Use of Ion Mobility Mass Spectrometry for Isomer Composition Determination Extracted from Se-Rich Yeast
peer reviewedThe isomer ratio determination of a selenium-containing metabolite produced by Se-rich yeast was performed. Electrospray Ionization and Ion Mobility Mass Spectrometry (IM-MS) were unsuccessfully used in order to resolve the isomers according to their Collisional Cross Section (CCS) difference. The isomer ratio determination of 2,3-dihydroxypropionylselenocystathionine was performed after multidimensional liquid chromatography preconcentration from a water extract of Se-rich yeast using preparative size exclusion, anion exchange and capillary reverse phase columns coupled to IM-MS. 4â-nitrobenzo-15-crown-5 ether, a Selective Shift Reagent (SSR), was added after the last chromatographic dimension in order to specifically increase the CCS of one of the isomers by the formation of a stable host-guest system with the crown-ether . Both isomers were consequently fully resolved by IM-MS and the relative ratio of the isomers was determined: 11-13% and 87-89%. The present data compared favorably with literature to support the analytical strategy despite the lack of authentic standard for method validation. In addition, computational chemistry methods were successfully applied to design the SSR and to support the experimental data