5 research outputs found
Exploring metabolic responses of potato tissue induced by electric pulses
In this study, we investigated the metabolic
responses of potato tissue induced by pulsed electric field
(PEF). Potato tissue was subjected to field strengths ranging
from 30 to 500 V/cm, with a single rectangular pulse of 10 μs,
100 μs, or 1 ms. Metabolic responses were monitored using
isothermal calorimetry, changes on electrical resistance during
the delivery of the pulse, as well as impedance measurements.
Our results show that the metabolic response involves oxygen
consuming pathways as well as other unidentified events that
are shown to be insensitive to metabolic inhibitors such as
KCN and sodium azide. The metabolic response is strongly
dependent on pulsing conditions and is independent of the
total permeabilization achieved by the pulse. Evidence shows
that calorimetry is a simple and powerful method for
exploring conditions for metabolic stimulation, providing
information on metabolic responses that can not be obtained
from electrical measurements. This study set the basis for
further investigations on defense-related consequences of
PEF-induced stress.Sparbanksstiftelsen Färs & Frosta (Sweden).Fundação para a Ciência e a Tecnologia (FCT).Lund University (Sweden).Department of Cell and Organism Biology; Department of Plant Biochemistry