Fermentation characteristics in hay from Cynodon and crop stubble treated with exogenous enzymes

ABSTRACT The effect of treatment with xylanase and β-glucanase was evaluated for gas production and the ruminal degradation of nutrients from the hay of Tifton 85 grass and the stubble of maize, sorghum, peanut, sunflower and sesame crops. Two commercial fibrolytic enzymes were used (Dyadic xylanase PLUS - Xylanase; BrewZyme LP-β-glucanase), added to the hay at doses of 7.5 units of endoglucanase and 0.46 units of xylanase per 500 mg/gDM, for the cellulase and xylanase products respectively. The chemical composition of the hay was determined for no enzyme application and 24 hours after enzyme treatment, and the in vitro gas production and in situ microbial degradation was estimated for dry matter, organic matter, neutral detergent fibre and truly-degradable organic matter after 24 hours of incubation in the rumen. Enzyme treatment of the hay from Tifton 85 grass and the stubble of maize, sorghum, sunflower, peanut and sesame crops with the exogenous fibrolytic enzymes β-glucanase and xylanase influences in vitro gas production, and the in situ degradation of dry matter, organic matter, neutral detergent fibre and truly-degradable organic matter in the rumen. This variation can be attributed to differences in the chemical composition of the hay from the grass and the crop stubble, and to the different ways the enzymes act upon the cell wall

Nutritive value of sheep diets made up of hay from Tifton 85 grass and leucaena

The intake, digestibility, nitrogen balance and energetic balance were evaluated in sheep diets consisting of hay from Tifton 85 grass at two ages of regrowth (28 and 42 days) and hay from leucaena at 40 days of regrowth, in the proportions of 20 and 40%. Twenty Santa Inês sheep, kept in metabolic cages, were used in a randomised block design according to body weight. The hay from the Tifton 85 grass, processed at 28 days of regrowth, resulted in a higher CP intake than when processed at 42 days of regrowth. There was higher protein intake for the 40% proportion of leucaena hay in relation to that of 20%, regardless of the regrowth age of the grass. Diets containing Tifton 85 grass processed at 28 days resulted in greater values for both DE and ME. There was greater intake, absorption and urinary excretion of N, and a lower Nfaecal to Nurinary ratio, with diets containing Tifton 85 grass processed at 28 days, as well as with those diets containing 40% leucaena hay. When formulating sheep diets containing hay from Tifton 85 grass processed at 28 days of regrowth and combined with 20% hay from leucaena, the grass, despite showing good energetic value, is not enough to provide full use of the nitrogen available to the ruminant, making it necessary need to find combinations with concentrated food sources which are able to optimise the use of nitrogen, reduce environmental impact and maximise sheep production