Improved method and case study of ground-coupled heat pump system design

AbstractIn this paper, the yearly performance of a library building located in Harbin, China, equipped with a ground-coupled heat pump (GCHP) system, was simulated and its ground heat exchanger (GHE) was designed in the TRNSYS 17 environment. Utilizing the hourly load simulation results, a TRNSYS model was developed to analyze underground thermal balance of the entire system, obtaining the 30-year operation conditions of the reference building. According to simulation results, annual average storage temperature, the highest and lowest inlet temperature of the heat pump, factors that influence efficiency of the system, change as the number of boreholes changes. And the optimized length of heat exchangers was studied, as well as method of determining the concentration of antifreeze in heat-transfer fluid in cold regions

Morin inhibits proliferation and self-renewal of CD133+ melanoma cells by upregulating miR-216a

Melanoma is one of the most malignant skin tumors with high mortality rate. Morin has been reported to treat several cancers. However, whether or how Morin affects melanoma progression is still poorly understood. Either Morin treatment or miR-216a overexpression reduced cell viability, sphere formation ability and expressions of stem cell marker genes CD20, CD44, CD133 and Wnt-3A. MiR-216a was induced by Morin treatment in CD133+ melanoma cells. Melanoma xenograft model treated by Morin showed reduced tumor size, weight as well as expressions of stemness markers and Wnt-3A. Inhibition of the stemness marker gene expressions in CD133+ melanoma cells is mediated by downregulating Wnt-3A through miR-216a. MiR-216a and Wnt-3A may potentially serve as clinical biomarkers of melanoma, and Morin may contribute to the treatment of melanoma. Keywords: Melanoma, Morin, CD133, miR-216a, Wnt-3

Complete plastid genome of Primula calliantha Franch. (Primulaceae): an alpine ornamental plant endemic to Hengduan Mountain, China

Primula calliantha Franch. is an alpine species with an ornamental value that is endemic to the Hengduan Mountains of southwest China. Here, we sequenced and assembled its plastid complete genome, which is a circular molecule of 151,954 bp and contains a large single-copy (83,820 bp) and a small single-copy (17,814 bp) region, separated by a pair of inverted repeats (25,160 bp). There are 131 genes, 86 protein-coding, 37 tRNAs, and 8 rRNAs in the plastome, of which 114 genes, 80 CDSs, 30 tRNAs, and 4 rRNAs are unique, respectively. The P. calliantha plastid genome shows a high level of synteny with its close relatives, P. chionantha, P. purdomii, and P. woodwardii. Phylogenetic analysis based on 60 complete chloroplast genomes of Primulaceae confirmed its delimitation in Primula

Viral Vector-Based Gene Therapy

Gene therapy is a technique involving the modification of an individual’s genes for treating a particular disease. The key to effective gene therapy is an efficient carrier delivery system. Viral vectors that have been artificially modified to lose their pathogenicity are used widely as a delivery system, with the key advantages of their natural high transduction efficiency and stable expression. With decades of development, viral vector-based gene therapies have achieved promising clinical outcomes. Currently, the three key vector strategies are based on adeno-associated viruses, adenoviruses, and lentiviruses. However, certain challenges, such as immunotoxicity and “off-target”, continue to exist. In the present review, the above three viral vectors are discussed along with their respective therapeutic applications. In addition, the major translational challenges encountered in viral vector-based gene therapies are summarized, and the possible strategies to address these challenges are also discussed

Attitude and influencing factors to receive the COVID‐19 vaccine among university students in Sichuan Province, China: A cross‐sectional study

Abstract Aims To explore the university students' attitude and the potential influencing factors to receive the coronavirus disease 2019 (COVID‐19) vaccine in Sichuan Province, China. Design A cross‐sectional study. Methods The self‐designed questionnaire was distributed among university students online in June 2021. SPSS software was used for statistical analysis of the data. Descriptive statistics, Chi‐square, two independent samples t‐tests, one‐way analysis of variance (ANOVA), multivariate linear regression, and content analysis were performed. Results A total of 397 questionnaires were analysed, involving 316 (79.6%) respondents have received at least one dose of a COVID‐19 vaccine and 81 (20.4%) have not taken the vaccine. The total mean score of university students' vaccination attitude was 25.97 (standard deviation [SD] = 3.720), and the total scoring rate was 74.2%. Main factors influencing students' attitude included education level, major, living style, with chronic disease or not, self‐reported vaccination status, and number of medical units that can provide vaccination within 3 km of residence. Students were more willing to choose Chinese‐manufactured vaccines (66.8%) and participate in collective vaccination programs organized by the school (71.3%). The desired vaccine protection period was 5–10 years (42.1%). The top three reasons for refusing the vaccine or vaccine hesitancy were as follows: concern about the side effects of vaccine (44.8%), lack of information about vaccine (31.0%), and concern about the efficacy of vaccine (29.3%). Conclusion In general, most of the participants had relatively high level of positive attitude to receive the COVID‐19 vaccine. Nevertheless, more attention should be paid to postgraduate students, non‐medical students, those living alone, those with chronic disease, those have not received the COVID‐19 vaccine, and those living far away from the vaccination medical units. Findings of this study can help educational institutions in developing effective interventions to improve the vaccination rate in the university student population

Post-translational modifications: The potential ways for killing cancer stem cells

While strides in cancer treatment continue to advance, the enduring challenges posed by cancer metastasis and recurrence persist as formidable contributors to the elevated mortality rates observed in cancer patients. Among the multifaceted factors implicated in tumor recurrence and metastasis, cancer stem cells (CSCs) emerge as noteworthy entities due to their inherent resistance to conventional therapies and heightened invasive capacities. Characterized by their notable abilities for self-renewal, differentiation, and initiation of tumorigenesis, the eradication of CSCs emerges as a paramount objective. Recent investigations increasingly emphasize the pivotal role of post-translational protein modifications (PTMs) in governing the self-renewal and replication capabilities of CSCs. This review accentuates the critical significance of several prevalent PTMs and the intricate interplay of PTM crosstalk in regulating CSC behavior. Furthermore, it posits that the manipulation of PTMs may offer a novel avenue for targeting and eliminating CSC populations, presenting a compelling perspective on cancer therapeutics with substantial potential for future applications