4 research outputs found
Integrative transcriptome and metabolome analysis reveals the mechanisms of light-induced pigmentation in purple waxy maize
IntroductionWaxy maize, mainly consumed at the immature stage, is a staple and vegetable food in Asia. The pigmentation in the kernel of purple waxy maize enhances its nutritional and market values. Light, a critical environmental factor, affects anthocyanin biosynthesis and results in pigmentation in different parts of plants, including in the kernel. SWL502 is a light-sensitive waxy maize inbred line with purple kernel color, but the regulatory mechanism of pigmentation in the kernel resulting in purple color is still unknown.MethodsIn this study, cyanidin, peonidin, and pelargonidin were identified as the main anthocyanin components in SWL502, evaluated by the ultra-performance liquid chromatography (UPLC) method. Investigation of pigment accumulation in the kernel of SWL502 was performed at 12, 17, and 22 days after pollination (DAP) under both dark and light treatment conditions via transcriptome and metabolome analyses.ResultsDark treatment affected genes and metabolites associated with metabolic pathways of amino acid, carbohydrate, lipid, and galactose, biosynthesis of phenylpropanoid and terpenoid backbone, and ABC transporters. The expression of anthocyanin biosynthesis genes, such as 4CL2, CHS, F3H, and UGT, was reduced under dark treatment. Dynamic changes were identified in genes and metabolites by time-series analysis. The genes and metabolites involved in photosynthesis and purine metabolism were altered in light treatment, and the expression of genes and metabolites associated with carotenoid biosynthesis, sphingolipid metabolism, MAPK signaling pathway, and plant hormone signal transduction pathway were induced by dark treatment. Light treatment increased the expression level of major transcription factors such as LRL1, myc7, bHLH125, PIF1, BH093, PIL5, MYBS1, and BH074 in purple waxy maize kernels, while dark treatment greatly promoted the expression level of transcription factors RVE6, MYB4, MY1R1, and MYB145.DiscussionThis study is the first report to investigate the effects of light on waxy maize kernel pigmentation and the underlying mechanism at both transcriptome and metabolome levels, and the results from this study are valuable for future research to better understand the effects of light on the regulation of plant growth
Ensemble Prediction Algorithm of Anomaly Monitoring Based on Big Data Analysis Platform of Open-Pit Mine Slope
With the diversification of pit mine slope monitoring and the development of new technologies such as multisource data flow monitoring, normal alert log processing system cannot fulfil the log analysis expectation at the scale of big data. In order to make up this disadvantage, this research will provide an ensemble prediction algorithm of anomalous system data based on time series and an evaluation system for the algorithm. This algorithm integrates multiple classifier prediction algorithms and proceeds classified forecast for data collected, which can optimize the accuracy in predicting the anomaly data in the system. The algorithm and evaluation system is tested by using the microseismic monitoring data of an open-pit mine slope over 6 months. Testing results illustrate prediction algorithm provided by this research can successfully integrate the advantage of multiple algorithms to increase the accuracy of prediction. In addition, the evaluation system greatly supports the algorithm, which enhances the stability of log analysis platform