A pair of new BAC and BIBAC vectors that facilitate BAC/BIBAC library construction and intact large genomic DNA insert exchange

<p>Abstract</p> <p>Background</p> <p>Large-insert BAC and BIBAC libraries are important tools for structural and functional genomics studies of eukaryotic genomes. To facilitate the construction of BAC and BIBAC libraries and the transfer of complete large BAC inserts into BIBAC vectors, which is desired in positional cloning, we developed a pair of new BAC and BIBAC vectors.</p> <p>Results</p> <p>The new BAC vector pIndigoBAC536-S and the new BIBAC vector BIBAC-S have the following features: 1) both contain two 18-bp non-palindromic I-<it>Sce</it>I sites in an inverted orientation at positions that flank an identical DNA fragment containing the <it>lac</it>Z selection marker and the cloning site. Large DNA inserts can be excised from the vectors as single fragments by cutting with I-<it>Sce</it>I, allowing the inserts to be easily sized. More importantly, because the two vectors contain different antibiotic resistance genes for transformant selection and produce the same non-complementary 3' protruding ATAA ends by I-<it>Sce</it>I that suppress self- and inter-ligations, the exchange of intact large genomic DNA inserts between the BAC and BIBAC vectors is straightforward; 2) both were constructed as high-copy composite vectors. Reliable linearized and dephosphorylated original low-copy pIndigoBAC536-S and BIBAC-S vectors that are ready for library construction can be prepared from the high-copy composite vectors pHZAUBAC1 and pHZAUBIBAC1, respectively, without the need for additional preparation steps or special reagents, thus simplifying the construction of BAC and BIBAC libraries. BIBAC clones constructed with the new BIBAC-S vector are stable in both <it>E. coli </it>and <it>Agrobacterium</it>. The vectors can be accessed through our website <url>http://GResource.hzau.edu.cn</url>.</p> <p>Conclusions</p> <p>The two new vectors and their respective high-copy composite vectors can largely facilitate the construction and characterization of BAC and BIBAC libraries. The transfer of complete large genomic DNA inserts from one vector to the other is made straightforward.</p

Possible Molecular Structure of the Newly Observed Y(4260)

We suggest that the newly observed resonance Y(4260) is a $\chi_{c}-\rho^0$ molecule, which is an isovector. In this picture, we can easily interpret why $Y(4260)\to \pi^+\pi^-J/\psi$ has a larger rate than $Y(4260)\to D\bar D$ which has not been observed, and we also predict existence of the other two components of the isotriplet and another two possible partner states which may be observed in the future experiments. A direct consequence of this structure is that for this molecular structure $Y(4260)\to \pi^+\pi^-J/\psi$ mode is more favorable than $Y(4260)\to K\bar KJ/\psi$ which may have a larger fraction if other proposed structures prevail.Comment: 5 pages, 2 figures. Some descriptions changed, more references added and typos corrected. Published version in PR

Synthesis and structures of O-anthrylmethyl-substituted hexahomotrioxacalix[3]arenes

O-Alkylation of 7,15,23-tri-tert-butyl-25,26,27-trihydroxy-2,3,10,11,18,19-hexahomo-3,11,19-trioxacalix[3]arene (1H₃) with 9-chloromethylanthracene 5 was carried out under different reaction conditions. Variation of the number of anthrylmethyl group introduced at the phenolic rim of hexahomotrioxacalix[3]arene 1H₃ was achieved through selective O-alkylation using stoichiometric amounts of 9-chloromethylanthracene 5 in acetone to afford the mono-O-alkylated product 2H₂An, the di-O-alkylated product 3HAn₂ and the tri-O-alkylated product partial-cone-4An₃, respectively. Interestingly, by using an acetone/benzene (1:1 v/v) mixed solvent system, the cone-4An₃ was successfully synthesized. These results suggest that the solvent can also control the conformation of the O-alkylation products. The possible reaction routes of the cone-4An₃ and partial-cone-4An₃ are also discussed