Combinatorial administration of insulin and vitamin C alleviates the cerebral vasospasm after experimental subarachnoid hemorrhage in rabbit

<p>Abstract</p> <p>Background</p> <p>Cerebral vasospasm (CVS) is a common serious complication after the spontaneous subarachnoid hemorrhage (SAH). Despite recent advances in medical and surgical treatments, the 30-day mortality rate of SAH remains high, and there is lack of especially effective clinical treatment to alleviate and improve CVS. The present study has investigated the therapeutic effect of insulin and vitamin C on CVS after SAH.</p> <p>Results</p> <p>Five days after SAH, there is obvious basilar artery spasm in SAH group, whose average vascular cross-sectional area (233,099 ± 16,750 μm²) is significantly smaller than that in control group (462,128 ± 74,756 μm²), which is also significantly different from those in SAH + insulin group (221,114 ± 43,457 μm²) and SAH + vitamin C group (237,820 ± 21,703 μm²). SAH + insulin + vitamin C group shows no evident vasospasm and maintains a vascular cross-sectional area of 425,530 ± 45,503 μm², which is significantly different from that in SAH group. Insulin receptor α (InRα) expression is significantly downregulated in the vascular endothelial cells of SAH, SAH + insulin, and SAH + vitamin C groups (<it>P </it>< 0.01) but remains unchanged in vascular endothelial cells of SAH + insulin + vitamin C group (<it>P </it>> 0.05). Five days after SAH, serum and cerebrospinal fluid NO levels in SAH, SAH + insulin, and SAH + vitamin C groups decrease significantly (<it>P </it>< 0.01) compared to that in control group, whereas the reduction is not evident in SAH + insulin + vitamin C group (<it>P </it>> 0.05).</p> <p>Conclusion</p> <p>Combinatorial treatment with insulin and vitamin C has effectively relieved the CVS after SAH in rabbit, possibly through increasing the InRα expression and NO level, whereas treatment with insulin or vitamin C alone fails to do so.</p

Applying the Cox Model to Study Online Gambling Behavior

Although a key objective of Internet gambling service providers is player retention, there is a concomitant need to reduce the social costs of gambling. Our study shows how habit and prospect theories help build an integrative framework for decision support in regulated Internet gambling environments. To illustrate the practical implication of this framework, we applied the Cox model with time-dependent covariates on real gambling data collected from 4,222 users of a gambling website. The results help establish the positive association of key indicators such as the prior outcomes on the activity lifespan of an Internet gambler and the moderating effect of gambling frequency on the positive association between prior outcomes and gambling lifespan. This research is expected to contribute to the literatures on IT adoption and diffusion in general, and IT-based addictive behavior in particular

A novel investment strategy for renewable-dominated power distribution networks

Aiming at the problem of insufficient adaptability to the new elements of the new power system in the current distribution network investment method, this paper innovatively proposes a distribution network investment method based on the new power system. By constructing a source-grid-load-storage-side investment calculation model, the investment in the new power system can be accurately calculated. First, the distributed power investment is calculated from the two aspects of new construction and renovation. Secondly, construct the grid investment demand and grid investment capacity measurement model, and obtain the grid side investment model by weighted summation. Then, a model for calculating the scale of investment that can be saved due to demand-side response is constructed, and the cost of demand response is subtracted to obtain a model for calculating the scale of investment that can be saved on the load side. Finally, the energy storage side investment calculation model is constructed from the power supply side, grid side, user-side energy storage investment, and energy storage investment benefit. The research results are applied to the empirical area, and scientific guidance is provided to realize the precise investment in the area

Generation of dual-gRNA library for combinatorial CRISPR screening of synthetic lethal gene pairs

Combinatorial CRISPR screening is useful for investigating synthetic lethality (SL) gene pairs. Here, we detail the steps for dual-gRNA library construction, with the introduction of two backbones, LentiGuide_DKO and LentiCRISPR_DKO. We describe steps fo

Spontaneous rotational symmetry breaking in KTaO3_3 interface superconductors

Strongly correlated electrons could display intriguing spontaneous broken symmetries in the ground state. Understanding these symmetry breaking states is fundamental to elucidate the various exotic quantum phases in condensed matter physics. Here, we report an experimental observation of spontaneous rotational symmetry breaking of the superconductivity at the interface of YAlO$_3$/KTaO$_3$ (111) with a superconducting transition temperature of 1.86 K. Both the magnetoresistance and upper critical field in an in-plane field manifest striking twofold symmetric oscillations deep inside the superconducting state, whereas the anisotropy vanishes in the normal state, demonstrating that it is an intrinsic property of the superconducting phase. We attribute this behavior to the mixed-parity superconducting state, which is an admixture of $s$-wave and $p$-wave pairing components induced by strong spin-orbit coupling. Our work demonstrates an unconventional nature of the pairing interaction in the KTaO$_3$ interface superconductor, and provides a new platform to clarify a delicate interplay of electron correlation and spin-orbit coupling.Comment: 7 pages, 4 figure