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Quantum entangled ground states of two spinor Bose-Einstein condensates
We revisit in detail the non-mean-field ground-state phase diagram for a
binary mixture of spin-1 Bose-Einstein condensates including quantum
fluctuations. The non-commuting terms in the spin-dependent Hamiltonian under
single spatial mode approximation make it difficult to obtain exact
eigenstates. Utilizing the spin z-component conservation and the total spin
angular momentum conservation, we numerically derive the information of the
building blocks and evaluate von Neumann entropy to quantify the ground states.
The mean-field phase boundaries are found to remain largely intact, yet the
ground states show fragmented and entangled behaviors within large parameter
spaces of interspecies spin-exchange and singlet-pairing interactions.Comment: 7 pages, 5 figure
Vacuolar ATPase depletion contributes to dysregulation of endocytosis in bloodstream forms of Trypanosoma brucei
BACKGROUND
Vacuolar H-ATPase (V-ATPase) is a highly conserved protein complex which hydrolyzes ATP and pumps protons to acidify vacuolar vesicles. Beyond its role in pH maintenance, the involvement of V-ATPase in endocytosis is well documented in mammals and plants but is less clear in Trypanosoma brucei.
METHODS
In this study, the subcellular localization of V-ATPase subunit B (TbVAB) of T. brucei was assessed via in situ N-terminal YFP-tagging and immunofluorescence assays. Transgenic bloodstream forms (BSF) of T. brucei were generated which comprised either a V-ATPase subunit B (TbVAB) conditional knockout or a V-ATPase subunit A (TbVAA) knockdown. Acridine orange and BCECF-AM were employed to assess the roles of V-ATPase in the pH regulation of BSF T. brucei. The endocytic activities of three markers were also characterized by flow cytometry analyses. Furthermore, trypanosomes were counted from trypanolysis treatment groups (either containing 1% or 5% NHS) and endocytosed trypanosome lytic factor (TLF) was also analyzed by an immunoblotting assay.
RESULTS
TbVAB was found to localize to acidocalcisomes, lysosomes and probably also to endosomes of BSF of T. brucei and was demonstrated to be essential for cell growth. TbVAB depletion neutralized acidic organelles at 24 hours post-tetracycline depletion (hpd), meanwhile the steady state intracellular pH increased from 7.016 ± 0.013 to 7.422 ± 0.058. Trypanosomes with TbVAB depletion at 24 hpd were found to take up more transferrin (2.068 ± 0.277 fold) but less tomato lectin (49.31 ± 22.57%) by endocytosis, while no significant change was detected in dextran uptake. Similar endocytic dysregulated phenotypes were also observed in TbVAA knockdown cells. In addition, TbVAB depleted trypanosomes showed a low uptake of TLF and exhibited less sensitive to lysis in both 1% and 5% NHS treatments.
CONCLUSIONS
TbVAB is a key component of V-ATPase and was found to play a key function in endocytosis as well as exhibiting different effects in a receptor/cargo dependent manner in BSF of T. brucei. Besides vacuolar alkalinization, the dysregulation of endocytosis in TbVAB depleted T. brucei is considered to contribute to the reduced sensitivity to lysis by normal human serum
Brueckner-Hartree-Fock and its renormalized calculations for finite nuclei
We have performed self-consistent Brueckner-Hartree-Fock (BHF) and its
renormalized theory to the structure calculations of finite nuclei. The
-matrix is calculated within the BHF basis, and the exact Pauli exclusion
operator is determined by the BHF spectrum. Self-consistent occupation
probabilities are included in the renormalized Brueckner-Hartree-Fock (RBHF).
Various systematics and convergences are studies. Good results are obtained for
the ground-state energy and radius. RBHF can give a more reasonable
single-particle spectrum and radius. We present a first benchmark calculation
with other {\it ab initio} methods using the same effective Hamiltonian. We
find that the BHF and RBHF results are in good agreement with other
methods
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