9 research outputs found
Pattern formation during the evaporation of a colloidal nanoliter drop: a numerical and experimental study
An efficient way to precisely pattern particles on solid surfaces is to
dispense and evaporate colloidal drops, as for bioassays. The dried deposits
often exhibit complex structures exemplified by the coffee ring pattern, where
most particles have accumulated at the periphery of the deposit. In this work,
the formation of deposits during the drying of nanoliter colloidal drops on a
flat substrate is investigated numerically and experimentally. A finite-element
numerical model is developed that solves the Navier-Stokes, heat and mass
transport equations in a Lagrangian framework. The diffusion of vapor in the
atmosphere is solved numerically, providing an exact boundary condition for the
evaporative flux at the droplet-air interface. Laplace stresses and thermal
Marangoni stresses are accounted for. The particle concentration is tracked by
solving a continuum advection-diffusion equation. Wetting line motion and the
interaction of the free surface of the drop with the growing deposit are
modeled based on criteria on wetting angles. Numerical results for evaporation
times and flow field are in very good agreement with published experimental and
theoretical results. We also performed transient visualization experiments of
water and isopropanol drops loaded with polystyrene microsphere evaporating on
respectively glass and polydimethylsiloxane substrates. Measured evaporation
times, deposit shape and sizes, and flow fields are in very good agreement with
the numerical results. Different flow patterns caused by the competition of
Marangoni loops and radial flow are shown to determine the deposit shape to be
either a ring-like pattern or a homogeneous bump
Control and ultrasonic actuation of a gas-liquid interface in a microfluidic chip
This article describes the design and manufacturing of a microfluidic chip,
allowing for the actuation of a gas-liquid interface and of the neighboring
fluid. A first way to control the interface motion is to apply a pressure
difference across it. In this case, the efficiency of three different
micro-geometries at anchoring the interface is compared. Also, the critical
pressures needed to move the interface are measured and compared to theoretical
result. A second way to control the interface motion is by ultrasonic
excitation. When the excitation is weak, the interface exhibits traveling
waves, which follow a dispersion equation. At stronger ultrasonic levels,
standing waves appear on the interface, with frequencies that are half integer
multiple of the excitation frequency. An associated microstreaming flow field
observed in the vicinity of the interface is characterized. The meniscus and
associated streaming flow have the potential to transport particles and mix
reagents
Acoustic excitation of superharmonic capillary waves on a meniscus in a planar micro-geometry
The effects of ultrasound on the dynamics of an air-water meniscus in a
planar micro-geometry are investigated experimentally. The sonicated meniscus
exhibits harmonic traveling waves or standing waves, the latter corresponding
to a higher ultrasound level. Standing capillary waves with subharmonic and
superharmonic frequencies are also observed, and are explained in the framework
of parametric resonance theory, using the Mathieu equation
Design of a novel flow-and-shoot microbeam
Presented here is a novel microbeam technology—the Flow-And-ShooT (FAST) microbeam—under development at RARAF. In this system, cells undergo controlled fluidic transport along a microfluidic channel intersecting the microbeam path. They are imaged and tracked in real-time, using a high-speed camera and dynamically targeted, using a magnetic Point and Shoot system. With the proposed FAST system, RARAF expects to reach a throughput of 100 000 cells per hour, which will allow increasing the throughput of experiments by at least one order of magnitude. The implementation of FAST will also allow the irradiation of non-adherent cells (e.g. lymphocytes), which is of great interest to many of the RARAF users. This study presents the design of a FAST microbeam and results of first tests of imaging and tracking as well as a discussion of the achievable throughput